Validation of a Salivary miRNA Diagnostic Test for ASD

NCT ID: NCT05418023

Last Updated: 2023-02-08

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

6604 participants

Study Classification

OBSERVATIONAL

Study Start Date

2022-03-16

Study Completion Date

2023-07-31

Brief Summary

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This study involves sample collection to identify biomarkers relating to Autism Spectrum Disorder(ASD) in the saliva of children who are between the ages of 18 months to 6 years and 11 months. Participants will at each timepoint have a non-invasive saliva swab collected and complete a brief demographic and medical history questionnaire as well. Children in the pediatric/provider setting who will receive a referral for an ASD diagnosis because they were determined to have a suspicion of developing ASD will be enrolled in the study. Children will also be enrolled in the specialist evaluation setting where they will receive a DSM-5 diagnosis. A subset of both enrollment cohorts will also be followed up with at a third time point in which their diagnosis will be confirmed, and information about any ongoing treatment will be gathered.

Detailed Description

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Conditions

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Autism Spectrum Disorder Developmental Delay

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

CROSS_SECTIONAL

Study Groups

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Autism Spectrum Disorder (ASD)

Salivary Collection

Intervention Type OTHER

Collection of saliva via swab for miRNA processing

Adaptive Assessment

Intervention Type OTHER

Includes Vineland Adaptive Behavior Scale or equivalent

Medical and Demographic questionnaire

Intervention Type OTHER

Survey that collects basic medical and demographic information

Autism Assessment

Intervention Type OTHER

Includes Autism Diagnostic Observation Schedule (ADOS), Childhood Autism Rating Scale (CARS), Gilliam Autism Rating Scale (GARS), Autism Diagnostic Interview - Revised (ADIR) or an equivalent

Intellectual Development Assessment

Intervention Type OTHER

Includes Mullens Scales of Early Learning, Stanford Binet Intelligence Scales, Wechsler Preschool and Primary Scale of Intelligence (WPPSI), Bayley Scales of Infant Development or an equivalent

Non-ASD

Salivary Collection

Intervention Type OTHER

Collection of saliva via swab for miRNA processing

Adaptive Assessment

Intervention Type OTHER

Includes Vineland Adaptive Behavior Scale or equivalent

Medical and Demographic questionnaire

Intervention Type OTHER

Survey that collects basic medical and demographic information

Autism Assessment

Intervention Type OTHER

Includes Autism Diagnostic Observation Schedule (ADOS), Childhood Autism Rating Scale (CARS), Gilliam Autism Rating Scale (GARS), Autism Diagnostic Interview - Revised (ADIR) or an equivalent

Intellectual Development Assessment

Intervention Type OTHER

Includes Mullens Scales of Early Learning, Stanford Binet Intelligence Scales, Wechsler Preschool and Primary Scale of Intelligence (WPPSI), Bayley Scales of Infant Development or an equivalent

Interventions

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Salivary Collection

Collection of saliva via swab for miRNA processing

Intervention Type OTHER

Adaptive Assessment

Includes Vineland Adaptive Behavior Scale or equivalent

Intervention Type OTHER

Medical and Demographic questionnaire

Survey that collects basic medical and demographic information

Intervention Type OTHER

Autism Assessment

Includes Autism Diagnostic Observation Schedule (ADOS), Childhood Autism Rating Scale (CARS), Gilliam Autism Rating Scale (GARS), Autism Diagnostic Interview - Revised (ADIR) or an equivalent

Intervention Type OTHER

Intellectual Development Assessment

Includes Mullens Scales of Early Learning, Stanford Binet Intelligence Scales, Wechsler Preschool and Primary Scale of Intelligence (WPPSI), Bayley Scales of Infant Development or an equivalent

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* 18 through 83 months old
* The study will include children "at risk" for ASD as defined by meeting one or more of the following criteria:

* flagged positive on a developmental screening tool (see below for assessment cut off scores)
* the child has a biological sibling with ASD
* Significant provider concern based on parent report noted in the child's medical chart at the time of the appointment.
* Significant parental concern

Exclusion Criteria

* Feeding tube dependence
* Active periodontal disease
* Confounding neurological condition (i.e. cerebral palsy, epilepsy)
* Sensory impairments (i.e. blindness or deafness)
* Acute illnesses (i.e. upper respiratory infection)
* Currently on antibiotics
* Had taken antibiotics within the previous 30 days
* Wards of the state
Minimum Eligible Age

18 Months

Maximum Eligible Age

7 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University of California, Irvine

OTHER

Sponsor Role collaborator

Holland Bloorview Kids Rehabilitation Hospital

OTHER

Sponsor Role collaborator

Children's Hospital of Philadelphia

OTHER

Sponsor Role collaborator

MetroHealth Medical Center

OTHER

Sponsor Role collaborator

University of Rochester

OTHER

Sponsor Role collaborator

Nationwide Children's Hospital

OTHER

Sponsor Role collaborator

Children's Hospital Medical Center, Cincinnati

OTHER

Sponsor Role collaborator

University of Pittsburgh

OTHER

Sponsor Role collaborator

University of Massachusetts, Worcester

OTHER

Sponsor Role collaborator

Children's Hospital Los Angeles

OTHER

Sponsor Role collaborator

Prisma Health-Upstate

OTHER

Sponsor Role collaborator

University of Virginia

OTHER

Sponsor Role collaborator

State University of New York - Upstate Medical University

OTHER

Sponsor Role collaborator

Rush University

OTHER

Sponsor Role collaborator

Seattle Children's Hospital

OTHER

Sponsor Role collaborator

Drexel University

OTHER

Sponsor Role collaborator

Emory University

OTHER

Sponsor Role collaborator

Quadrant Biosciences Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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David Levitskiy, MS

Role: PRINCIPAL_INVESTIGATOR

Quadrant Biosciences

Locations

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University of California - Irvine

Santa Ana, California, United States

Site Status RECRUITING

Marcus Autism Center/Emory University

Atlanta, Georgia, United States

Site Status RECRUITING

RUSH University

Chicago, Illinois, United States

Site Status NOT_YET_RECRUITING

University of Massachusetts Medical School

Worcester, Massachusetts, United States

Site Status RECRUITING

University of Rochester

Rochester, New York, United States

Site Status RECRUITING

Quadrant Biosciences

Syracuse, New York, United States

Site Status ACTIVE_NOT_RECRUITING

SUNY Upstate Medical University

Syracuse, New York, United States

Site Status NOT_YET_RECRUITING

Cincinnati Children's Hospital Medical Center

Cincinnati, Ohio, United States

Site Status RECRUITING

MetroHealth Medical Center

Parma, Ohio, United States

Site Status RECRUITING

Nationwide Children's Hospital

Westerville, Ohio, United States

Site Status RECRUITING

Children's Hospital of Philadelphia

Philadelphia, Pennsylvania, United States

Site Status RECRUITING

Drexel University

Philadelphia, Pennsylvania, United States

Site Status RECRUITING

Prisma Health-Upstate

Greenville, South Carolina, United States

Site Status RECRUITING

Texas Children's Hospital/Baylor College of Medicine

Houston, Texas, United States

Site Status RECRUITING

University of Virginia

Charlottesville, Virginia, United States

Site Status RECRUITING

Seattle Children's Hospital

Seattle, Washington, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Andrew Brindle

Role: CONTACT

3153260760

Arianna Montefusco

Role: CONTACT

3153260760

Facility Contacts

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Jean Gehricke, PhD

Role: primary

Cheryl Klaiman, PhD

Role: primary

Latha Soorya, PhD

Role: primary

Jean Frazier, MD

Role: primary

Lynn Cole, MS

Role: primary

Henry Roane, PhD

Role: primary

Rebecca Shaffer, PsyD

Role: primary

Melissa Armstrong-Brine, PhD

Role: primary

Eric Butter, PhD

Role: primary

Amanda Bennett, MD

Role: primary

Diana Robins, PhD

Role: primary

Karen Ratliff-Schaub, MD

Role: primary

Robin Kochel, PhD

Role: primary

Richard Stevenson, MD

Role: primary

Karen Bearss, PhD

Role: primary

References

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Hicks SD, Ignacio C, Gentile K, Middleton FA. Salivary miRNA profiles identify children with autism spectrum disorder, correlate with adaptive behavior, and implicate ASD candidate genes involved in neurodevelopment. BMC Pediatr. 2016 Apr 22;16:52. doi: 10.1186/s12887-016-0586-x.

Reference Type BACKGROUND
PMID: 27105825 (View on PubMed)

Hicks SD, Rajan AT, Wagner KE, Barns S, Carpenter RL, Middleton FA. Validation of a Salivary RNA Test for Childhood Autism Spectrum Disorder. Front Genet. 2018 Nov 9;9:534. doi: 10.3389/fgene.2018.00534. eCollection 2018.

Reference Type BACKGROUND
PMID: 30473705 (View on PubMed)

Hicks SD, Carpenter RL, Wagner KE, Pauley R, Barros M, Tierney-Aves C, Barns S, Greene CD, Middleton FA. Saliva MicroRNA Differentiates Children With Autism From Peers With Typical and Atypical Development. J Am Acad Child Adolesc Psychiatry. 2020 Feb;59(2):296-308. doi: 10.1016/j.jaac.2019.03.017. Epub 2019 Mar 27.

Reference Type BACKGROUND
PMID: 30926572 (View on PubMed)

Other Identifiers

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20214744

Identifier Type: -

Identifier Source: org_study_id

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