cfDNA Assay Prospective Observational Validation for Early Cancer Detection and Minimal Residual Disease

NCT ID: NCT05366881

Last Updated: 2026-01-20

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

7000 participants

Study Classification

OBSERVATIONAL

Study Start Date

2022-05-03

Study Completion Date

2027-03-31

Brief Summary

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This is an observational case-control study to train and validate a genome-wide methylome enrichment platform to detect multiple cancer types and to differentiate amongst cancer types. The cancers included in this study are brain, breast, bladder, cervical, colorectal, endometrial, esophageal, gastric, head and neck, hepatobiliary, leukemia, lung, lymphoma, multiple myeloma, ovarian, pancreatic, prostate, renal, sarcoma, and thyroid. These cancers were selected based on their prevalence and mortality to maximize impact on clinical care.

Additionally, the ability of the whole-genome methylome enrichment platform to detect minimal residual disease after completion of cancer treatment and to detect relapse prior to clinical presentation will be evaluated in lung cancer. This cancer was selected based on the existing clinical landscape and treatment availability.

Detailed Description

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This is an observational case-control study that includes individuals with cancer and individuals without known cancer. All participants will have clinical follow-up after enrollment. A subset of individuals with cancer will also have longitudinal blood sampling to evaluate the ability of the genome-wide methylome enrichment platform to detect minimal residual disease. This includes individuals with Stage I-III lung cancer (Tier 1 Cancers).

At baseline, all participants will provide a blood sample and applicable clinical data.

Participants with a Tier 1 cancer will have clinical follow-up and blood draws after the completion of first-line treatment, every 3 months for the first year after first-line treatment, and every 6 months for an additional 2 years. All other cases may have clinical follow-up once a year for 3 years after enrollment.

Control participants will have clinical follow-up every 6 months for up to 3 years from enrollment to evaluate cancer status.

The blood test to be used in this study is a highly sensitive, epigenomic-based genome-wide methylome enrichment platform. The assay includes bisulfite-free, non-degradative genome-wide DNA methylation profiling from small quantities of cell-free DNA (cfDNA). Libraries constructed from cfDNA are enriched for methylated CpGs and preserve the native fragment length. This is followed by high throughput sequencing.

For all assays, samples from participants with cancer and participants without cancer will be run together to reduce batch effects using methodology determined by the Sponsor. Results from the liquid biopsy test will not be returned to clinicians or participants.

Conditions

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Brain Cancer Breast Cancer Bladder Cancer Cervical Cancer Colorectal Cancer Endometrial Cancer Esophageal Cancer Stomach Cancer Head and Neck Cancer Hepatobiliary Cancer Leukemia Lung Cancer Lymphoma Multiple Myeloma Ovarian Cancer Pancreatic Cancer Prostate Cancer Renal Cancer Sarcoma Thyroid Cancer

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Cases

Cases will include participants with newly diagnosed, treatment-naive cancer at the time of enrollment.

No interventions assigned to this group

Controls

Controls will include participants without known cancer at the time of enrollment.

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

* Newly diagnosed (within 120 days) with cancer or a recurrence of a cancer diagnosed \>5 years ago of one of the following subtypes: Invasive Brain, Breast, Bladder, Cervical, Colorectal, Endometrial, Esophageal, Gastric, Head and Neck, Hepatobiliary, Lung, Ovarian, Pancreatic, Prostate, Renal, Sarcoma, Thyroid; Leukemia, Lymphoma, Multiple Myeloma
* Able and willing to provide informed consent
* ≥40 years of age


* Not diagnosed with any cancer in the last 5 years (non-invasive cancer is allowed)
* Able and willing to provide informed consent
* ≥40 years of age

Exclusion Criteria

* Currently receiving any treatment for cancer
* Currently taking any demethylating agents/DNA hypomethylating agents
* Simultaneously diagnosed with two or more invasive cancers
* Diagnosed with any invasive or non-invasive cancer in addition to the index cancer in the last 5 years
* Currently diagnosed with any chronic hematopoietic cancer (e.g. chronic CLL) in addition to the index cancer
* Currently diagnosed with any myelodysplastic syndromes and/or precursor hematologic conditions (e.g. MGUS) in addition to the index cancer
* Women who are known to be pregnant (self-reported)


* Currently receiving any treatment for cancer
* Currently taking any demethylating agents/DNA hypomethylating agents
* Women who are known to be pregnant (self-reported)
Minimum Eligible Age

40 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Adela, Inc

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Brian Rini, MD

Role: PRINCIPAL_INVESTIGATOR

Vanderbilt-Ingram Cancer Center

Locations

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City of Hope

Duarte, California, United States

Site Status RECRUITING

Miami Cancer Institute

Miami, Florida, United States

Site Status RECRUITING

North Georgia Health System

Gainesville, Georgia, United States

Site Status RECRUITING

Baptist Floyd

New Albany, Indiana, United States

Site Status RECRUITING

Baptist Corbin

Corbin, Kentucky, United States

Site Status RECRUITING

Baptist Hardin

Elizabethtown, Kentucky, United States

Site Status RECRUITING

Baptist Lexington

Lexington, Kentucky, United States

Site Status RECRUITING

Baptist Paducah

Paducah, Kentucky, United States

Site Status WITHDRAWN

Allina Health Cancer Institute

Minneapolis, Minnesota, United States

Site Status RECRUITING

Mayo Clinic

Rochester, Minnesota, United States

Site Status RECRUITING

Cleveland Clinic

Cleveland, Ohio, United States

Site Status RECRUITING

Oregon Health Sciences University

Portland, Oregon, United States

Site Status RECRUITING

Medical University of South Carolina

Charleston, South Carolina, United States

Site Status RECRUITING

McLeod Health

Florence, South Carolina, United States

Site Status RECRUITING

Baptist (BHMCC)

Memphis, Tennessee, United States

Site Status RECRUITING

Vanderbilt-Ingram Cancer Center

Nashville, Tennessee, United States

Site Status RECRUITING

Elligo Health Research, Inc.

Austin, Texas, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Brian Allen, MS

Role: CONTACT

203-514-4155

Michelle Anderson

Role: CONTACT

475-766-8183

Facility Contacts

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Xiao-Yu Xia

Role: primary

626-218-0630

Belkys Salinas

Role: primary

Trena Davis

Role: primary

Tasha Dodson

Role: primary

Janie Platt

Role: primary

Amy Steward

Role: primary

Janie Platt

Role: primary

Jonathon Alexander

Role: primary

612-863-8625

Jennie Schletty

Role: backup

612-863-8625

Angela R Emanuel

Role: primary

Mofetoluwa Oluwasanmi

Role: primary

216-444-0843

Diana Potts

Role: primary

Michael Balassone

Role: primary

843-792-6696

Andrew M Chen

Role: backup

Betsy Harris

Role: primary

Tracy Stewart

Role: primary

Anna Dumont

Role: primary

Cynthia Currasco

Role: primary

Other Identifiers

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Adela-EDMRD-001

Identifier Type: -

Identifier Source: org_study_id

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