Effect of HPV Integration on Prognosis of Young Women With CIN2 in China

NCT ID: NCT05282095

Last Updated: 2024-09-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

300 participants

Study Classification

OBSERVATIONAL

Study Start Date

2022-06-01

Study Completion Date

2026-05-31

Brief Summary

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Clinically, cervical precancerous lesion is one of the important diseases that endanger the life safety and fertility of young women. Women with histopathologically confirmed CIN2 need regular HPV, cervical cytology, and colposcopic biopsy if necessary to assess the outcome and progression of the disease. In this study, we intend to visit Fujian Maternal and Child Health Hospital, Tongji Hospital affiliated to Tongji Medical College of Huazhong University of Science \& Technology and other hospitals, including 300 CIN2 participants aged 45 and below diagnosed by histopathology, and collect the remaining cervical secretions and cervical exfoliated cell samples after clinical examination, even if you do not participate in this clinical study. In clinical diagnosis, treatment and follow-up, it is also necessary to collect the above specimens for relevant medical tests. Therefore, it is of great clinical and scientific significance to explore the role of HPV integrated detection in predicting the prognosis of young women with CIN2.

Detailed Description

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This study aims to:1) determine the correlation between HPV integration and natural outcome in young CIN2 women. 2) determine the prognostic value of different HPV integration status in young women with CIN2. 3) determine the relationship between the integration status of different HPV genes in young CIN2 women and the results of vaginal flora and cervical exfoliative cytology. A prospective cohort of 300 participants under 45 years of age with histopathologically confirmed CIN2 was recruited from multiple centers, and HPV integration status, HPV infection status, cervical cytology, and vaginal flora diversity sequencing were performed at enrollment, 3 months, 6 months, 9 months, and 12 months. The purpose of this study was to evaluate the effect of HPV integration status and flora changes on the outcome and progression of CIN2 women, and to evaluate the correlation between HPV integration status and cervical cytology results.

Conditions

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Cervical Intraepithelial Neoplasia HPV Infection Virus Integration HSIL, High Grade Squamous Intraepithelial Lesions

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Women aged from 18-45 with histopathologically confirmed CIN2

In the enrollment, women whose cervical histopathological results have been diagnosed as cervical intraepithelial neoplasia (CIN2) for the last 3 months with abnormal results will be included in this study. All participants will be followed up four, at 3th month, 6th month, 9th month and 12th month.

Follow up

Intervention Type OTHER

Participants will be followed up at baseline, 3th, 6th, 9th and 12th months with HPV viral integration tests, HPV genotyping tests, thinprep cytologic tests (TCT) for cervix and vaginal secretion tests. Colposcopies and biopsies will be performed on the participants at 6 - and 12-month follow-up.

Interventions

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Follow up

Participants will be followed up at baseline, 3th, 6th, 9th and 12th months with HPV viral integration tests, HPV genotyping tests, thinprep cytologic tests (TCT) for cervix and vaginal secretion tests. Colposcopies and biopsies will be performed on the participants at 6 - and 12-month follow-up.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Female, 18 years of age or older and 45 years of age or younger, with a desire to conceive;
* Diagnosed with HSIL (CIN2) or HSIL (CIN2-3) via cervical tissue biopsy within the past 3 months, and has not undergone cervical surgery, physical, or medication treatment;
* The lesion area under colposcopy is less than 50% of the total cervical area within the past 3 months;
* Plans for 12-month follow-up observation for CIN2, with no surgical, physical, or medication treatment if the disease does not progress;
* Understands and voluntarily agrees to participate in the 12-month follow-up of this study, and signs the informed consent form.

Exclusion Criteria

* Cervical status at the time of enrollment as determined by colposcopy within the past three months is Type III transformation zone;
* Pregnant or lactating;
* History of malignant reproductive tract tumors;
* History of hysterectomy, cervical surgery, or pelvic radiation therapy;
* Physical therapy to the cervix within 24 months prior to enrollment;
* The subject has a severe immune system disease that is active;
* Long-term use of contraceptives within 12 months prior to enrollment;
* Vaginal medication or irrigation within 72 hours prior to sampling (can re-enroll for sampling 3 days after cessation);
* Sexual intercourse within 24 hours prior to sampling (can re-enroll for sampling 24 hours after cessation);
* Received treatment for genital tract infections, HPV, or other STD pathogens within the past month (can re-enroll one month after cessation of treatment);
* Used antibiotics or vaginal microecological improvement products within the past month (can re-enroll one month after cessation of use);
Maximum Eligible Age

45 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Fujian Maternity and Child Health Hospital

OTHER

Sponsor Role lead

Responsible Party

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Binhua Dong

Principal Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Pengming Sun

Role: STUDY_CHAIR

Fujian Maternal and Child Health Hospital

Locations

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Fujian Maternity and Child Health Hospital

Fuzhou, Fujian, China

Site Status RECRUITING

Fujian Medical University Union Hospital

Fuzhou, Fujian, China

Site Status RECRUITING

The First Affiliated Hospital of Fujian Medical University

Fuzhou, Fujian, China

Site Status RECRUITING

Longyan First Hospital

Longyan, Fujian, China

Site Status RECRUITING

The Second Hospital of Longyan

Longyan, Fujian, China

Site Status RECRUITING

Ningde Municipal Hospital of Ningde Normal University

Ningde, Fujian, China

Site Status RECRUITING

The First Hospital of Putian City

Putian, Fujian, China

Site Status RECRUITING

The Affiliated Hospital of Putian University

Putian, Fujian, China

Site Status RECRUITING

Sanming Second Hospital

Sanming, Fujian, China

Site Status RECRUITING

Zhangzhou affiliated Hospital of Fujian Medical University

Zhangzhou, Fujian, China

Site Status RECRUITING

Gansu Provincial Maternal and Child Health Care Hospital

Lanzhou, Gansu, China

Site Status RECRUITING

Shenzhen Maternity and Child HealthCare Hospital

Shenzhen, Guangdong, China

Site Status RECRUITING

Guiyang maternal and child health care hospital

Guiyang, Guizhou, China

Site Status RECRUITING

Hubei Maternal and Child Health Care Hospital

Wuhan, Hubei, China

Site Status RECRUITING

Tongji Hospital affiliated to Tongji Medical College of Huazhong University of Science & Technology

Wuhan, Hubei, China

Site Status RECRUITING

Zhongnan Hospital of Wuhan University

Wuhan, Hubei, China

Site Status RECRUITING

Jiangxi maternal and Child Health Hospital

Nanchang, Jiangxi, China

Site Status RECRUITING

Shanghai First Maternity and Infant Hospital

Shanghai, Shanghai Municipality, China

Site Status RECRUITING

Northwest Women's and Children's Hospital

Xi’an, Shanxi, China

Site Status RECRUITING

The First Affiliated Hospital of Ningbo University

Ningbo, Zhejiang, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Binhua Dong

Role: CONTACT

+86-591-87558732

Pengming Sun

Role: CONTACT

+86-591-87558732

Facility Contacts

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Binhua Dong

Role: primary

+8613599071900

Peifang Chen

Role: primary

Lihong Chen

Role: primary

Jinyong Wang

Role: primary

Wenrong Huang

Role: primary

Wenfang Jin

Role: primary

+8618559336833

Xianqian Chen

Role: primary

+8618559336833

Lijun Li

Role: primary

Yisheng Lin

Role: primary

Feifeng Shi, M.D

Role: primary

+8613960086066

Xunyuan Tuo

Role: primary

Zheng Zheng

Role: primary

Juntao Wang

Role: primary

Hongning Cai

Role: primary

Yan Li

Role: primary

Wei Zhang

Role: primary

Ling Li

Role: primary

Xiaowen Pu

Role: primary

Hongwei Tan

Role: primary

Yutao Guan

Role: primary

References

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Zhang Y, Zhang Y, Hu T, Pu X, Dong B, Tuo X, Zou H, Zhang W, Lyu Q, Huang W, Xue H, Xu S, Osafo KS, Ren Y, Lin W, Su J, Huang X, Sun P. Effect of HPV integration on prognosis of young women with CIN2 in China: protocol for a multicentre prospective cohort study. BMJ Open. 2025 Apr 28;15(4):e093863. doi: 10.1136/bmjopen-2024-093863.

Reference Type DERIVED
PMID: 40295124 (View on PubMed)

Other Identifiers

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HPVZHCIN22021

Identifier Type: -

Identifier Source: org_study_id

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