Immunological Effect of Increasing Doses of Natural Peptide

NCT ID: NCT05176678

Last Updated: 2022-06-09

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

24 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-11-04

Study Completion Date

2023-07-11

Brief Summary

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A trial monitoring the immune effects in 24 people with 4 different doses (1 gram per day, 2 grams per day, 4 grams per day, 8 grams per day) of a natural plant-based protein hydrolysate.

Detailed Description

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The goal for this clinical proof-of-concept study is to compare immune effects of a novel plant-based peptide on IL-17, Th17 and related immunological mechanisms.

An open-label, escalating dose study design monitoring immune effects in which healthy men and women take doses of a natural protein hydrolysate daily for 6 weeks.

During the first 2 weeks, participants will consume 1 gram/day. For the next 2 weeks, participants will consume 2 grams/day. For the final 2 weeks, participants will be randomly assigned to consume either 4 grams/day or 8 grams/day.

Blood samples are collected at baseline and every 2 weeks. The blood will be used for testing of the numbers of T lymphocytes that secrete Interleukin-17 after ex vivo cell cultures.

Conditions

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Pharmacokinetics

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SEQUENTIAL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Escalating dose of natural protein hydrolysate - sequence A

Participants will consume escalating doses of natural protein hydrolysate: Week 1-2: 1 gram/day, Week 3-4: 2 gram/day, Week 5-6: 4 gram/day.

Group Type ACTIVE_COMPARATOR

1 gram of a protein hydrolysate

Intervention Type DIETARY_SUPPLEMENT

After 1 gram of a protein hydrolysate is taken daily for 2 weeks, 1 blood draw will be taken.

2 grams of a protein hydrolysate

Intervention Type DIETARY_SUPPLEMENT

After 2 grams of a protein hydrolysate is taken daily for 2 weeks, 1 blood draw will be taken.

4 grams of a protein hydrolysate

Intervention Type DIETARY_SUPPLEMENT

After 4 grams of a protein hydrolysate is taken daily for 2 weeks, 1 blood draw will be taken.

Escalating dose of natural protein hydrolysate - sequence B

Participants will consume escalating doses of natural protein hydrolysate: Week 1-2: 1 gram/day, Week 3-4: 2 gram/day, Week 5-6: 8 gram/day.

Group Type ACTIVE_COMPARATOR

1 gram of a protein hydrolysate

Intervention Type DIETARY_SUPPLEMENT

After 1 gram of a protein hydrolysate is taken daily for 2 weeks, 1 blood draw will be taken.

2 grams of a protein hydrolysate

Intervention Type DIETARY_SUPPLEMENT

After 2 grams of a protein hydrolysate is taken daily for 2 weeks, 1 blood draw will be taken.

8 grams of a protein hydrolysate

Intervention Type DIETARY_SUPPLEMENT

After 8 grams of a protein hydrolysate is taken daily for 2 weeks, 1 blood draw will be taken.

Interventions

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1 gram of a protein hydrolysate

After 1 gram of a protein hydrolysate is taken daily for 2 weeks, 1 blood draw will be taken.

Intervention Type DIETARY_SUPPLEMENT

2 grams of a protein hydrolysate

After 2 grams of a protein hydrolysate is taken daily for 2 weeks, 1 blood draw will be taken.

Intervention Type DIETARY_SUPPLEMENT

4 grams of a protein hydrolysate

After 4 grams of a protein hydrolysate is taken daily for 2 weeks, 1 blood draw will be taken.

Intervention Type DIETARY_SUPPLEMENT

8 grams of a protein hydrolysate

After 8 grams of a protein hydrolysate is taken daily for 2 weeks, 1 blood draw will be taken.

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* 24 men and women
* Eating a typical diet that does not exceed an estimated average of 9 grams of salt per day;
* Willing to comply with a 24-hour wash-out period for vitamins and nutritional supplements;
* Willing to comply with study procedures including: Maintaining a consistent diet and lifestyle routine throughout the study, Consistent habit of bland breakfasts on days of clinic visits, Abstaining from exercising and nutritional supplements on the morning of a study visit, Abstaining from use of coffee, tea, and soft drinks for at least one hour prior to a clinic visit; Abstaining from music, candy, gum, computer/cell phone use, during clinic visits.

Exclusion Criteria

* Previous major gastrointestinal surgery Vaccination during past month;
* Vaccination planned during study;
* Cancer during past 12 months;
* Chemotherapy during past 12 months;
* Diagnosed with an autoimmune disorder;
* Diagnosed with obstructive sleep apnea syndrome (OSAS);
* Currently taking medication containing Levodopa (L-dopa);
* Having received a cortisone shot within the past 6 months;
* Taking anti-inflammatory medications on a daily basis;
* Currently experiencing intense stressful events/life changes;
* Currently in intensive athletic training (such as marathon runners);
* An unusual sleep routine
* Changes to nutritional supplements during the past month;
* Currently taking Vitamin D at a dose higher than 1,000 IU/day;
* Currently taking omega-3 at a dose higher than 1 gram/day;
* Unwilling to maintain a constant intake of supplements over the duration of the study;
* Women of childbearing potential: Pregnant, nursing, or trying to become pregnant;
* Known food intolerances related to ingredients in active test product.
Minimum Eligible Age

25 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Natural Immune Systems Inc

OTHER

Sponsor Role lead

Responsible Party

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Gitte Jensen, Ph.D.

Research Director

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Gitte S Jensen

Role: PRINCIPAL_INVESTIGATOR

NIS Labs

Locations

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Gitte Jensen

Klamath Falls, Oregon, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Gitte S Jensen, PhD

Role: CONTACT

541-884-0112

Facility Contacts

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Gitte S Jensen, PhD

Role: primary

541-884-0112

Other Identifiers

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NIS 184-001

Identifier Type: -

Identifier Source: org_study_id

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