Impact of Exercise and Hyperlipidic Meal on Free Circulating DNA in Patients With Metastatic Colonic Cancer and Healthy Subjects

NCT ID: NCT05147545

Last Updated: 2023-04-18

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

60 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-09-30

Study Completion Date

2024-06-30

Brief Summary

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The study of circulating tumoral DNA makes it possible to study, without invasive procedures or pathological studies, the tumoral DNA circulating in the blood of a patient and its various alterations. In patients with colon-rectal cancer with resected tumor, circulating tumor DNA can be used as a predictive biomarker of metastatic relapse of cancer. However, the routine extension of circulating tumoral DNA remains limited due to several difficulties. One of the pifalls that circulating tumor DNA is greatly diluted by healthy circulating DNA from non-tumor cells. The amount of healthy circulating DNA has been described as being influenced by certain physiological parameters. The aim of the study is to increase knowledge on the influence of physiological factors associated with sports activity and meal on the release kinetics of circulating DNA.

Detailed Description

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20 subjects free of malignancy (Male-Female Ratio 2 to 1) the variation in the concentration of DNA circulating between the value measured on an empty stomach after 1 hour of rest, then at the end of each of the successive stages of moderate intensity physical effort: pedaling 15 minutes at 75 Watt then 100 Watt of the ergometer. Dosages will be repeated after 15, 30 and 60 minutes of recovery. After an hour of recovery, the subject will be asked to consume a meal rich in fat. The circulating DNA will be measured 2 hours after the end of the meal.

40 patients with colon cancer will be enrolled for a two visit study. The first visit will be planned at the first cycle of chemotherapy, before the chemotherapy. At this visit, the influence of exercise on plasma concentrations of circulating free DNA will also be studied in 40 patients with colon cancer. Free circulating DNA will be measured on an empty stomach after 1 hour of rest, then immediately after low-intensity physical effort : pedaling 3 minutes at the 30 Watt level of the ergometer) and after 15, 30 and 60 minutes of recovery. After an hour of recovery, the subject will be proposed to consume a meal rich in fat. The circulating DNA will be measured 2 hours after the end of the meal. The second visit will be planned at the first second of chemotherapy, before the chemotherapy. Free circulating DNA will be measured on an empty stomach after 1 hour of rest, then immediately after low-intensity physical effort : pedaling 3 min at the 30 Watt level of the ergometer, and after 15, 30 and 60 minutes of recovery.

Conditions

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Colon Cancer

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Physiological pilot study-two groups
Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Subjects free of malignancy

Moderate intensity physical effort and meal (once)

Group Type EXPERIMENTAL

Moderate intensity physical effort

Intervention Type OTHER

Pedaling 15 minutes at 75 Watt then 100 Watt of the ergometer

Hyperlipidic meal

Intervention Type OTHER

Eating an hyperlipidic meal 60 minutes after a physical effort

Measure of circulating DNA

Intervention Type DIAGNOSTIC_TEST

Multiple measure of circulating DNA: on an empty stomach, at the end of the physical effort, after 15, 30 and 60 minutes of rest, 120 minutes after an hyperlipidic meal.

An additional measure will occur for subjects free of malignancy during the effort.

Patients with colon cancer

Low intensity physical effort and meal (visit 1, day of cycle 1 chemotherapy) and low intensity physical effort (visit 2, day of cycle 2 chemotherapy)

Group Type EXPERIMENTAL

Low intensity physical effort

Intervention Type OTHER

Pedaling 3 minutes at the 30 Watt level of the ergometer

Hyperlipidic meal

Intervention Type OTHER

Eating an hyperlipidic meal 60 minutes after a physical effort

Measure of circulating DNA

Intervention Type DIAGNOSTIC_TEST

Multiple measure of circulating DNA: on an empty stomach, at the end of the physical effort, after 15, 30 and 60 minutes of rest, 120 minutes after an hyperlipidic meal.

An additional measure will occur for subjects free of malignancy during the effort.

Interventions

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Moderate intensity physical effort

Pedaling 15 minutes at 75 Watt then 100 Watt of the ergometer

Intervention Type OTHER

Low intensity physical effort

Pedaling 3 minutes at the 30 Watt level of the ergometer

Intervention Type OTHER

Hyperlipidic meal

Eating an hyperlipidic meal 60 minutes after a physical effort

Intervention Type OTHER

Measure of circulating DNA

Multiple measure of circulating DNA: on an empty stomach, at the end of the physical effort, after 15, 30 and 60 minutes of rest, 120 minutes after an hyperlipidic meal.

An additional measure will occur for subjects free of malignancy during the effort.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

1. Subjects free of malignancy

* men or women 40-70 yrs aged
2. Patients with colon cancer

* men or women 18-85 yrs aged
* Biopsy-proven colon cancer with indication to chemiotherapy
* Chemiotherapy (first line or second line) not started

Exclusion Criteria

1. All subjects

* Ischemic cardiac history
* known heart disease
* blood hemoglobin concentration \<8g / dl
* Acute or chronic systemic illnesses, (apart from diabetes, essential or secondary hypertension for patients)
* Pregnancy or breastfeeding or in progress
2. Subjects free of malignancy

* Cardiovascular risk factor (active smoking or greater than 10 pack-years, diabetes, hypertension or known dyslipidemia not controlled by the diet)
* Glomerular filtration rate estimated by the CKD epi (Chronic Kidney Disease - Epidemiology Collaboration) formula \< 60 ml.min-1.1.73 m-²
* Contraindication to exercise
* Drug taking in progress (except estrogen-progestogen contraception)
Minimum Eligible Age

18 Years

Maximum Eligible Age

85 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Eurofins Biomnis

OTHER

Sponsor Role collaborator

Assistance Publique - Hôpitaux de Paris

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Pierre LAURENT-PUIG, MD-PhD

Role: PRINCIPAL_INVESTIGATOR

Assistance Publique - Hôpitaux de Paris

Locations

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Assistance Publique Hôpitaux de Paris, Hôpital européen Georges Pompidou

Paris, Île-de-France Region, France

Site Status

Countries

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France

Central Contacts

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Annie BERGERA

Role: CONTACT

33 1 44 84 17 24

Facility Contacts

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Pierre LAURENT-PUIG, MD-PHD

Role: primary

331 76 53 43 81

Other Identifiers

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IDRCB 2020-A02872-37

Identifier Type: OTHER

Identifier Source: secondary_id

APHP201461

Identifier Type: OTHER

Identifier Source: secondary_id

APHP201461

Identifier Type: -

Identifier Source: org_study_id

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