A Biomarker-Guided, Randomized, Placebo-Controlled Efficacy and Safety Study of Liafensine in Patients With TRD

NCT ID: NCT05113771

Last Updated: 2025-05-15

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE2
• Total Enrollment: 197 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2022-06-29
• Study Completion Date: 2024-03-05

Brief Summary

This study was conducted as a randomized, double-blind, placebo-controlled, multi-center Phase 2b study. Approximately 180 subjects with treatment resistant depression who meet all eligibility criteria will be enrolled. The primary endpoint is to demonstrate liafensine is superior to placebo in DGM4 positive patients with TRD.

Detailed Description

This study is a randomized, double blind, placebo-controlled Phase 2b study to assess the efficacy, safety, tolerability, and pharmacokinetics of liafensine. Eligible patients were randomized 1:1:1 to receive liafensine 1 mg QD, liafensine 2 mg QD, or placebo QD. The main objectives of this study are as follows:

Primary Efficacy Objective: To demonstrate that liafensine was superior to placebo in DGM4 positive patients with TRD as assessed by the change in MADRS total score from baseline to Day 42 of double blind treatment

Key Secondary Efficacy Objective: To evaluate the change from baseline to Day 42 in DGM4 positive patients with TRD treated with liafensine vs placebo on the Clinical Global Impression-Severity Scale (CGI S)

Other Secondary Efficacy Objective: To evaluate the Clinical Global Impression-Improvement Scale (CGI I) at Day 42 in DGM4 positive patients with TRD treated with liafensine vs placebo

Safety Objective: To compare the safety and tolerability of liafensine vs placebo in all randomized patients with TRD who received at least one dose of study drug during double blind treatment

Psychiatric assessments were performed by a psychiatrist or trained and certified clinical staff member. Neurologic assessments were performed by an experienced clinician. Patients who fulfilled Hy's Law, defined as ALT or AST ≥ 3 × ULN and TBL ≥ 2 × ULN, in the absence of significant increase in ALP and in the absence of an alternative diagnosis that explained the increase in total bilirubin, were discontinued, with medical follow up as appropriate.

Conditions

Treatment Resistant Depression

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: TREATMENT
• Blinding Strategy: DOUBLE

Study Groups

Liafensine 1mg

Patients with TRD were treated with liafensine 1 mg QD for 6 weeks.

Group Type: EXPERIMENTAL

Liafensine

Intervention Type: DRUG

Liafensine

Liafensine 2mg

Patients with TRD were treated with liafensine 2 mg QD for 6 weeks.

Group Type: EXPERIMENTAL

Liafensine

Intervention Type: DRUG

Liafensine

Placebo

Patients with TRD were treated with placebo 1 mg QD for 6 weeks.

Group Type: PLACEBO_COMPARATOR

Placebo

Intervention Type: DRUG

Placebo

Interventions

Liafensine

Liafensine

Intervention Type: DRUG

Placebo

Placebo

Intervention Type: DRUG

Other Intervention Names

DB104

Eligibility Criteria

Inclusion Criteria

1. Provide signed informed consent which includes pharmacogenomic (PGx) testing.

2. Have a diagnosis of MDD without psychotic features, according to the Diagnostic and Statistical Manual of Mental Disorders, 5th Edition (DSM-5) criteria, based on clinical assessment and confirmed by the Mini International Neuropsychiatric Interview (MINI).

3. Have a history of TRD within the past 5 years as documented by the Massachusetts General Hospital (MGH) Antidepressant Treatment Response Questionnaire (ATRQ) (5-year version). That is, within the past 5 years study participants must have had a clinically meaningful inadequate response (estimated < 50% improvement per Investigator/patient consensus and documented by the Investigator) to at least two treatment courses with antidepressant regimens. These must involve at least two different pharmacologic treatment classes* and have been given at accepted therapeutic doses for an adequate duration (at least 6 weeks). One of these treatment failures must have occurred within the current episode.

*Note: Non-pharmacological treatment (eg, cognitive behavioral therapy, electroconvulsive therapy, repetitive transcranial magnetic stimulation, vagus nerve stimulation, acupuncture) are not counted as treatment regimen.

4. To be eligible, patients must have DGM4 genotype results obtained from the designated Clinical Laboratory Improvement Amendments (CLIA) lab, and all eligible DGM4-positive patients and about 20% DGM4-negative patients will be randomly included by an IRT system in order to achieve the appropriate randomization ratio of DGM4-positive vs negative patients.

5. Pregnancy conception limitations

• Female patients must be postmenopausal or surgically sterile or, if of childbearing potential and the partner is not vasectomized (6 months minimum), must agree to use a medically acceptable form of contraception from the time of signing the informed consent form (ICF) through at least 60 days following the last administration of study drug. If only the barrier method is used, a double barrier must be employed. Postmenopausal women must have had ≥ 24 months of spontaneous amenorrhea. Surgically sterile women are defined as those who have had a hysterectomy, bilateral ovariectomy, or bilateral tubal ligation. All women of childbearing potential must have a negative pregnancy test result before administration of study drug.
• Male patients must be biologically incapable of having children (eg, vasectomized) or must agree to use the above forms of birth control for themselves and their partner from the time of signing the informed consent form through at least 120 days following the last administration of study drug.

6. Be fluent in the local language.

7. Male or female aged 18 to 70, inclusive, at time of enrollment.

8. Have a HAMD-17 total score ≥ 21 at screening.

9. Be willing to discontinue the use of antidepressant drugs (including over-the-counter medications to treat depression [eg, St John's Wort]) at least 5 half-lives (or at least 1 week for herbal or other over-the-counter medications for depression) prior to baseline (Day -1). For fluoxetine, a washout period of at least 3 weeks for ≤ 20 mg/day and at least 4 weeks for > 20 mg/day is required.

Exclusion Criteria

1. Prior participation in a study with liafensine

2. Used any investigational drug product, device, or biologic within 6 months or five half-lives (whichever is longer) prior to baseline (Day -1).

3. A positive pregnancy test result or currently breastfeeding.

4. Clinically significant illness (including chronic, persistent, or acute infection), medical/surgical procedure, or trauma within 30 days prior to screening or between screening and baseline (Day 1) as determined by the investigator.

5. A history or presence of a clinically significant hepatic, renal, gastrointestinal, cardiovascular, endocrine, respiratory, immunologic, hematologic, dermatologic, or neurologic abnormality, or any other condition, that in the investigator's opinion, represent potential risk to the patient's safety, full participation in the study, or affect the absorption, distribution, metabolism, or excretion of liafensine.

6. Presence of autoimmune hepatitis, primary sclerosing cholangitis, untreated hepatitis C, active hepatitis B, or any other uncontrolled or unstable liver disease according to local guidance.

7. Uncontrolled human immunodeficiency virus (HIV) infection according to local guidance.

8. Uncontrolled abnormal thyroid function according to local guidance.

9. One or more clinical laboratory evaluations are outside the reference range, at screening, that are in the investigator's opinion, of potential risk to the patient's safety.

10. Has at the Screening Visit:

• Alanine aminotransferase (ALT) or aspartate aminotransferase (AST) levels > 1.5x the upper limit of normal (ULN) at screening.
• Total bilirubin (TBL) > 2 mg/dL (34.2 μmol/L) at screening, unless there is an explained indirect hyperbilirubinemia, eg, Gilbert's syndrome.
• Alkaline phosphatase (ALP) > 1.5x the ULN at screening. Note: Laboratory tests can be repeated to see if values return to normal range, but any such laboratory abnormality must be resolved by the Baseline Visit (Day -1).

11. Clinically significant vital sign abnormality at screening. This includes, but is not limited to, the following, in the supine (after at least 5 min rest) and standing (after 1 min and 3 min standing): systolic blood pressure ≥ 140 mmHg; diastolic blood pressure ≥ 90 mmHg; or heart rate < 50 or > 90 beats per minute. If the initial blood pressure is ≥ 140/90 mmHg, the lowest value from up to 3 additional attempts, which also must not be ≥ 140/90 mmHg, should be used. Patients with symptomatic orthostatic hypotension, at the discretion of investigator, will be excluded.

12. Corrected QT interval measurement according to the Fridericia rule (QTcF) > 450 msec for men and > 470 msec for women during controlled rest at screening, or history of long-QT syndrome.

13. ECGs containing any of the following readings:

• Left bundle branch block
• Right bundle branch block with QRS duration > 140 ms
• Intraventricular conduction defect with QRS duration > 140 ms
• Long QT syndrome

14. History of seizure, other than childhood febrile seizures.

15. History of clinically significant head trauma, including closed head injury with loss of consciousness, that is, in the opinion of the investigator, likely to affect central nervous system function.

16. History of clinically significant symptomatic orthostatic hypotension (ie, postural syncope).

17. History of narrow angle glaucoma.

18. History of cancer within 2 years prior to screening or between screening and baseline (Day -1), except for non-metastatic basal and/or squamous cell carcinoma of the skin.

19. Use of prescription or nonprescription medications for attention-deficit hyperactivity disorder (ADHD), narcolepsy, or cognitive enhancement (eg, methylphenidate, atomoxetine, modafinil, ginkgo biloba, and huperzine A) within 30 days prior to screening or between screening and baseline (Day -1).

20. Regular consumption of (eg, more days than not) excessive quantities of xanthine-containing beverages (eg, more than five cups of coffee or the equivalent per day) within 30 days prior to screening or between screening and baseline (Day -1).

21. Urine drug screen (UDS) positive for a drug of abuse, with the exception of cannabis in countries where it is legally available (see Table 3 for list of drugs of abuse). Where legal, prior use of cannabis is permitted provided the patient agrees to abstain from smoking or ingesting cannabis or cannabis products during the study.

22. Use of potent inducers of CYP3A4 (eg, rifampin, rifabutin, phenytoin, carbamazepine, or phenobarbital) within 2 weeks prior to baseline (Day-1).

23. Current diagnosis or history of a psychotic disorder, MDD with psychotic features, manic or hypomanic episode of bipolar or related disorders.

24. Current diagnosis of anxiety disorder (if primary), post-traumatic stress disorder, obsessive compulsive disorder (if primary), intellectual disability (DSM-5 diagnostic code 319), borderline personality disorder, antisocial personality disorder, histrionic personality disorder, or narcissistic personality disorder according to the DSM-5 criteria, or any other psychiatric or neurologic disorder or symptom due to a general medical condition, that, in the judgement of the investigator, could pose undue risk to the patient or compromise the study.

25. Hospitalized or discharged from psychiatric ward within 8 weeks prior to the screening visit and planned hospitalization for any condition(s) during the study.

26. Moderate or severe alcohol use disorder or other substance use disorder (except nicotine or caffeine), within 6 months prior to screening, according to the DSM-5 criteria.

27. Significant risk of suicide determined by:

1. Acute suicidality as evidenced by answering "yes" to Question 5 ("In the Past Year") on the C-SSRS, indicating active suicidal ideation with specific plan and intent for suicide, at screening, or baseline (Day -1); or

2. History of suicidal behavior as indicated by a "yes" response on the Suicidal Behavior section of the C-SSRS ("In the past year") or

3. A score ≥ 5 on Item 10 (suicidal thoughts) of the MADRS at screening or baseline (Day -1); or

4. Has attempted suicide within 6 months prior to the initial screening visit.

28. Previous allogenic bone marrow transplant.

29. Received non-leukocyte-depleted whole blood transfusion within 4 months prior to PGx testing at Screening.

30. Currently employed by the sponsor or by a clinical trial site participating in this study, or a first-degree relative of an employee of the sponsor or of an employee at a participating clinical trial site.

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 70 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Denovo Biopharma LLC

INDUSTRY

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Matthew A Spear, M.D.

Role: STUDY_DIRECTOR

Denovo Biopharma

Locations

University of Alabama at Birmingham

Huntsville, Alabama, United States

Alea Research

Phoenix, Arizona, United States

Collaborative Neuroschience Research, LLC

Garden Grove, California, United States

Behavioral Research Specialists, LLC

Glendale, California, United States

Sunwise Clinical Research, LLC.

Lafayette, California, United States

Excell Research

Oceanside, California, United States

Anderson Clinical Reseach

Redlands, California, United States

Schuster Medical Research Institute

Sherman Oaks, California, United States

Collaborative Neuroscience Research, LLC

Torrance, California, United States

Pacific Clinical Research Management Group

Upland, California, United States

Clinical Research of Brandon, LLC

Brandon, Florida, United States

Access Research Institute

Brooksville, Florida, United States

The Medicine Medical Research

Hollywood, Florida, United States

Nuovida Research Center

Miami, Florida, United States

SG Research, LLC

Miami, Florida, United States

Aqualane Clinical Research

Naples, Florida, United States

Clinical Neuroscience Solutions, Inc.

Orlando, Florida, United States

CenExel Atlanta Center for Medical Research

Atlanta, Georgia, United States

Psych Atlanta, PC

Marietta, Georgia, United States

Revive Research Institute, Inc

Elgin, Illinois, United States

Ascension Via Christi Research, a division of Ascension Via Christi Hospitals Wichita, Inc.

Wichita, Kansas, United States

Pharmasite Research, Inc.

Baltimore, Maryland, United States

CBH Health LLC

Gaithersburg, Maryland, United States

Boston Clinical Trials & Medical Research

Boston, Massachusetts, United States

Neurobehavioral Medicine Group

Bloomfield, Michigan, United States

Alivation Research, LLC

Lincoln, Nebraska, United States

Altea Research Institute, Las Vegas

Las Vegas, Nevada, United States

Hassman Research Institute

Marlton, New Jersey, United States

Global Medical Institutes, LLC

Princeton, New Jersey, United States

Global Medical Institutes, LLC

Princeton, New Jersey, United States

Bio Behavior Health

Toms River, New Jersey, United States

IMA Clinical Research

Albuquerque, New Mexico, United States

Zucker Hillside Hospital

Glen Oaks, New York, United States

The Ohio State University Department of Psychiatry

Columbus, Ohio, United States

Lehigh Center for Clinical Research, LLC

Allentown, Pennsylvania, United States

Global Medical Institutes, LLC

Moosic, Pennsylvania, United States

FutureSearch Trials of Dallas

Dallas, Texas, United States

InSite Clinical Research

DeSoto, Texas, United States

North Texas Clinical Trials

Fort Worth, Texas, United States

University of Texas Medical School at Houston

Houston, Texas, United States

Core Clinical Research

Everett, Washington, United States

OCT Research ULC

Kelowna, British Columbia, Canada

AMNDX Inc.

Markham, Ontario, Canada

St. Michael's Hospital

Toronto, Ontario, Canada

CAMH-Russell Street Site 250 College Street

Toronto, Ontario, Canada

Countries

United States; Canada

References

Wang G, Aguado M, Spear MA, Alphs L, Chen C, Huang H, Lu XX, Doostzadeh J, Wu S, Wang S, Patel A, Nemeroff CB, Wang Z, Li A, Luo W. ANK3 as a Novel Genetic Biomarker for Liafensine in Treatment-Resistant Depression: The ENLIGHTEN Randomized Clinical Trial. JAMA Psychiatry. 2025 Sep 10:e252416. doi: 10.1001/jamapsychiatry.2025.2416. Online ahead of print.

Reference Type: DERIVED

PMID: 40928787 (View on PubMed)

Provided Documents

Document Type: Study Protocol

View Document

Document Type: Statistical Analysis Plan

View Document

Other Identifiers

DB104-01

Identifier Type: -

Identifier Source: org_study_id