Spike-specific Cellular Immune Response After COVID-19 Vaccination

NCT ID: NCT05102669

Last Updated: 2024-04-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

53 participants

Study Classification

OBSERVATIONAL

Study Start Date

2021-03-12

Study Completion Date

2021-10-11

Brief Summary

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Aim of this study is to evaluate whether COVID-19 vaccination induces a persistent cellular immune response.

To this aim, blood samples are taken from vaccinated individuals and not immunized subjects as a control group.

Cells isolated from blood samples are tested in vitro to assess the percentage of spike-specific T and B lymphocytes 1 and 7 months after a second dose of Comirnaty vaccine.

Detailed Description

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After providing written informed consent, 28 vaccinated and 25 non vaccinated subjects were included in the study. All subjects were enrolled among healthcare workers in the Research Centre of IRCCS San Raffaele Roma in Rome.

All the subjects declared that they never tested positive for COVID19. Blood and serum samples were drowned twice from vaccinated subjects: 1 and 7 months after a second dose of vaccine to evaluate T and B response to vaccine; blood was drowned once in not vaccinated subjects.

Cells were collected form peripheral blood samples, while anti-S1 IgG titre was assessed in serum.

Flow-cytometry was used to assess the percentage of circulating spike-specific T and B lymphocytes.

Conditions

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COVID19 Vaccination Immune Response

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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vaccinated

COVID19 vaccinated subjects

analysis of immunological response

Intervention Type OTHER

collection and analysis of SARS-CoV2 Spike specific blood cells

analysis of serum antibodies

Intervention Type DIAGNOSTIC_TEST

analysis of anti-SARS-CoV2 Spike specific antibodies

non vaccinated

COVID19 non vaccinated subjects

analysis of immunological response

Intervention Type OTHER

collection and analysis of SARS-CoV2 Spike specific blood cells

Interventions

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analysis of immunological response

collection and analysis of SARS-CoV2 Spike specific blood cells

Intervention Type OTHER

analysis of serum antibodies

analysis of anti-SARS-CoV2 Spike specific antibodies

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* vaccinated and non vaccinated subjects
* subjects that never tested positive for COVID19

Exclusion Criteria

* subjects that tested positive for COVID-19
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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IRCCS San Raffaele Roma

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Laura Vitiello, PhD

Role: PRINCIPAL_INVESTIGATOR

IRCCS San Raffaele Roma

Locations

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IRCCS San Raffaele Roma

Roma, , Italy

Site Status

Countries

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Italy

Other Identifiers

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RP 20/09

Identifier Type: -

Identifier Source: org_study_id

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