Predictors of Disease Severity in COVID-19 Patients

NCT ID: NCT04887142

Last Updated: 2021-05-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

1019 participants

Study Classification

OBSERVATIONAL

Study Start Date

2020-04-04

Study Completion Date

2023-05-31

Brief Summary

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The primary goal of the VIRIONUM project is to analyze the association of clinical, socio-demographic characteristics of patients and their genetic polymorphism, inflammatory and other biochemical markers with disease progression and death in subjects with COVID-19 disease. The research is an observational, cohort study with nested-case control design, including adult, male and female COVID-19 inpatients. Primary dependent variable will be outcome of disease, defined as discharge from the hospital or death. The outcomes and putative risk factors will be analyzed using binary logistic regressions. The proposed multi- and inter-disciplinary study should provide additional scientific evidence about risk factors for the development of severe forms of the disease and the COVID-19-related death.

Detailed Description

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Background: Severe acute respiratory syndrome 2 (SARS-CoV-2) is etiological agent of heterogeneous corona-virus disease 19 (COVID-19), which clinical condition range from asymptomatic or mild to critically severe with high odds for fatal outcome. Since the pathophysiological pathways of COVID-19 disease development are not fully elucidated, resulting in repeated changes in treatment plans, there is an unmet need for more research into genetic, biochemical, immunological, and clinical predictive indicators of better outcomes in COVID-19 patients.

Aim: The VIRIONUM project's main aim is to investigate the relation between genetic polymorphisms, inflammatory and other biochemical markers, and disease severity and mortality in COVID-19-infected hospitalized patients.

Method and patients: The observational nested case-control clinical study will be conducted in at least 1019 COVID-19 patients. The genetic polymorphisms (ACE2, IFNL3/4 and TMPRSS2), serum concentration of soluble ACE2 and the pro- and anti-inflammatory cytokines, blood concentration of common inflammatory and other biomarkers, peripheral blood mononuclear leukocyte (PBMC) phenotype, demographic and clinical risk factors will be monitored as independent variables, while the dependent variables will be the outcome of the disease and the severity of the clinical condition. Genotyping will be performed by Real-Time PCR method, and serum concentrations of ACE2 and pro- and anti-inflammatory cytokines by ELISA method, and PBMC phenotype by flow cytometry. The disease severity will be assessed according to WHO criteria.

Expected results: The findings of this clinical study will contribute to understanding of significant genetic, biochemical, and clinical determinants of severity and lethal outcomes in COVID-19 patients, as well as, creation of set of recommendations for individuals at higher risk - future, evidence-based, targeted and individualized approach.

Conditions

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COVID-19

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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The discharged subjects

The COVID-19 inpatients treated with standard care who survived the disease and discharged from hospital.

Standard of health care

Intervention Type OTHER

The patients in this observational study will be exposed to the standard health care interventions according to the hospital diagnostic and treatment protocols and procedures and clinical judgment of the treating physicians.

The deceased subjects

The COVID-19 inpatients treated with standard care who died from the disease.

Standard of health care

Intervention Type OTHER

The patients in this observational study will be exposed to the standard health care interventions according to the hospital diagnostic and treatment protocols and procedures and clinical judgment of the treating physicians.

Interventions

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Standard of health care

The patients in this observational study will be exposed to the standard health care interventions according to the hospital diagnostic and treatment protocols and procedures and clinical judgment of the treating physicians.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Adult age, 18 years old or more
* Male or female
* Hospitalization for COVID-19 after confirmation by SARS-CoV-2 rapid antigen test or RT-PCR
* Voluntary written consent of the subject to participate

Exclusion Criteria

* Pregnant and breastfeeding women
* Fatal outcome during the first 24 hours of hospitalization
* Inability to collect the necessary study data
* Declining of the subject to voluntary participate in the study
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Zeljko Mijailovic

OTHER

Sponsor Role lead

Responsible Party

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Zeljko Mijailovic

Principal Investigator

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Dejan Baskic, MD PHD

Role: STUDY_CHAIR

Faculty of Medical Sciences

Locations

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Spitali Amerikan

Tirana, , Albania

Site Status NOT_YET_RECRUITING

General Hospital "Prim. dr. Abdulah Nakaš"

Sarajevo, , Bosnia and Herzegovina

Site Status NOT_YET_RECRUITING

KBC Rijeka, University of Rijeka

Rijeka, , Croatia

Site Status NOT_YET_RECRUITING

University of Montenegro, Faculty of Medicine

Podgorica, , Montenegro

Site Status NOT_YET_RECRUITING

Faculty of Medical Sciences, University Clinical Center Kragujevac

Kragujevac, Srbija, Serbia

Site Status RECRUITING

Countries

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Albania Bosnia and Herzegovina Croatia Montenegro Serbia

Central Contacts

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Zeljko Mijailovic, MD PhD

Role: CONTACT

+381 64 1675986

Dragan Milovanovic, MD PhD

Role: CONTACT

+38134505050 ext. 5254

Facility Contacts

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Lindita Mano, MD PhD

Role: primary

+355692077045

Edin Begic, MD

Role: primary

+387 33 285 100

Igor Barkovic, MD PhD

Role: primary

+385915374094

Aneta Boskovic, MD PhD

Role: primary

+382 20 246651

Dragan Milovanovic, MD PhD

Role: primary

+381652977831

Other Identifiers

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01/20-405

Identifier Type: -

Identifier Source: org_study_id

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