Study of mRNA Vaccine Formulation Against COVID-19 in Healthy Adults 18 Years of Age and Older

NCT ID: NCT04798027

Last Updated: 2025-09-11

Study Results

Results available

Outcome measurements, participant flow, baseline characteristics, and adverse events have been published for this study.

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Basic Information

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Recruitment Status

TERMINATED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

182 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-03-12

Study Completion Date

2022-06-27

Brief Summary

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The primary objectives of the study are:

* To describe the safety profile of all participants in each age group and each study intervention group up to 12 months post-last dose.
* To describe the neutralizing antibody profile at Day 1, Day 22, and Day 36 of each study intervention group.

The secondary objectives of the study are:

* To describe binding antibody profile from Day 1 to Day 387 of each study intervention group.
* To describe the neutralizing antibody profile from Day 91 to Day 387 of each study intervention group.
* To describe the occurrence of virologically-confirmed coronavirus disease-2019 (COVID-19)-like illness and serologically-confirmed SARS-CoV-2 infection.
* To evaluate the correlation/association between antibody responses to SARS-CoV-2 messenger RNA (mRNA) vaccine and the risk of virologically-confirmed COVID-19-like illness and/or serologically-confirmed severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection.

Detailed Description

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The duration of each participant's participation in the study was approximately 365 days post-last injection: approximately 386 days duration for participants receiving 2 injections and approximately 365 days duration total for participants receiving a single injection.

Conditions

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COVID-19

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

This was a sequential group prevention study consisting of a sentinel cohort followed by the Full Enrollment Cohort. There were 3 dose levels (up to 25 participants 18-49 years of age for each dose level) in the Sentinel Cohort, which were done in an open-label fashion with stepwise safety evaluation for each dose level and each vaccination. All sentinel participants received 2 vaccinations, 21 days apart. For the Full Enrollment Cohort (FEC), which was done in a double-blind design participants were stratified into 2 groups based on age at enrollment: the younger adult age group (140 planned participants 18-49 years of age) and the older adult age group (168 planned participants greater than or equal to \[\>=\] 50 years of age). The FEC Cohort 1 (Groups 1 to 3) received a single injection of study intervention while participants in Cohort 2 (Groups 4 to 6) received 2 vaccinations (to be given 21 days apart).
Primary Study Purpose

PREVENTION

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors
In the FEC: blinding for vaccine group assignment (formulation) of participants, outcome assessors, Investigators, laboratory personnel, and Sponsor study staff. Only study site staff who prepare and administer the vaccine and were not involved with the safety evaluations were unblinded to vaccine group assignment. There was no blinding for injection schedule.

The Sentinel Cohort was open-label (no blinding).

Study Groups

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Sentinel Cohort: SARS-CoV-2 Vaccine Ultra Low dose

Participants received two intramuscular (IM) injections of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Vaccine ultra-low dose on Day 1 and at Day 22, respectively.

Group Type EXPERIMENTAL

SARS-CoV-2 mRNA vaccine formulation 1

Intervention Type BIOLOGICAL

Pharmaceutical form: Sterile suspension Route of administration: Intramuscular injection

Sentinel Cohort: SARS-CoV-2 Vaccine Low dose

Participants received two IM injections of SARS-CoV-2 Vaccine low dose on Day 1 and at Day 22, respectively.

Group Type EXPERIMENTAL

SARS-CoV-2 mRNA vaccine formulation 2

Intervention Type BIOLOGICAL

Pharmaceutical form: Sterile suspension Route of administration: Intramuscular injection

Sentinel Cohort: SARS-CoV-2 Vaccine Medium dose

Participants received two IM injections of SARS-CoV-2 Vaccine medium dose on Day 1 and at Day 22, respectively.

Group Type EXPERIMENTAL

SARS-CoV-2 mRNA vaccine formulation 3

Intervention Type BIOLOGICAL

Pharmaceutical form: Sterile suspension Route of administration: Intramuscular injection

FEC Cohort 1: SARS-CoV-2 Vaccine Ultra Low dose

Participants received a single IM injection of SARS-CoV-2 Vaccine ultra-low dose on Day 1.

Group Type EXPERIMENTAL

SARS-CoV-2 mRNA vaccine formulation 1

Intervention Type BIOLOGICAL

Pharmaceutical form: Sterile suspension Route of administration: Intramuscular injection

FEC Cohort 1: SARS-CoV-2 Vaccine Low dose

Participants received a single IM injection of SARS-CoV-2 Vaccine low dose on Day 1.

Group Type EXPERIMENTAL

SARS-CoV-2 mRNA vaccine formulation 2

Intervention Type BIOLOGICAL

Pharmaceutical form: Sterile suspension Route of administration: Intramuscular injection

FEC Cohort 1: Placebo

Participants received a single IM injection of placebo matched to SARS-CoV-2 Vaccine on Day 1.

Group Type PLACEBO_COMPARATOR

Placebo (0.9% normal saline)

Intervention Type BIOLOGICAL

Pharmaceutical form: Liquid Route of administration: Intramuscular injection

FEC Cohort 2: SARS-CoV-2 Vaccine Ultra Low dose

Participants received two IM injections of SARS-CoV-2 Vaccine ultra-low dose on Day 1 and at Day 22, respectively.

Group Type EXPERIMENTAL

SARS-CoV-2 mRNA vaccine formulation 1

Intervention Type BIOLOGICAL

Pharmaceutical form: Sterile suspension Route of administration: Intramuscular injection

FEC Cohort 2: SARS-CoV-2 Vaccine Low dose

Participants received two IM injections of SARS-CoV-2 Vaccine low dose on Day 1 and at Day 22, respectively.

Group Type EXPERIMENTAL

SARS-CoV-2 mRNA vaccine formulation 2

Intervention Type BIOLOGICAL

Pharmaceutical form: Sterile suspension Route of administration: Intramuscular injection

FEC Cohort 2: Placebo

Participants received two IM injections of placebo matched to SARS-CoV-2 Vaccine on Day 1 and at Day 22, respectively.

Group Type PLACEBO_COMPARATOR

Placebo (0.9% normal saline)

Intervention Type BIOLOGICAL

Pharmaceutical form: Liquid Route of administration: Intramuscular injection

Interventions

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SARS-CoV-2 mRNA vaccine formulation 1

Pharmaceutical form: Sterile suspension Route of administration: Intramuscular injection

Intervention Type BIOLOGICAL

SARS-CoV-2 mRNA vaccine formulation 2

Pharmaceutical form: Sterile suspension Route of administration: Intramuscular injection

Intervention Type BIOLOGICAL

SARS-CoV-2 mRNA vaccine formulation 3

Pharmaceutical form: Sterile suspension Route of administration: Intramuscular injection

Intervention Type BIOLOGICAL

Placebo (0.9% normal saline)

Pharmaceutical form: Liquid Route of administration: Intramuscular injection

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* Aged \>= 18 years on the day of inclusion.
* A female participant was eligible to participate if she was not pregnant or breastfeeding and one of the following conditions applies:
* Was of non-childbearing potential. To be considered of non-childbearing potential, a female must be pre-menarche or post-menopausal for at least 1 year, or surgically sterile.

OR

* Was of childbearing potential and agreed to use an effective contraceptive method or abstinence from at least 4 weeks prior to the first vaccination until at least 12 weeks after the last vaccination.

A participant of childbearing potential must had a negative highly sensitive pregnancy test (urine or serum as required by local regulation) within 8 hours before the study intervention.

* Informed Consent Form had been signed and dated.
* Participant not eligible to receive, based on local guidance, or if eligible does not intend to receive an authorized/approved COVID-19 vaccine from first vaccination until completion of the key timepoint of Day 43 of follow-up of this study.

Exclusion Criteria

* History of COVID-19 disease or prior SARS-CoV-2 infection confirmed serologically.
* Known or suspected congenital or acquired immunodeficiency; or receipt of immunosuppressive therapy, such as anti-cancer chemotherapy or radiation therapy, within the preceding 6 months; or long-term systemic corticosteroid therapy (prednisone or equivalent for more than 2 consecutive weeks within the past 3 months).
* Chronic illness or condition considered to potentially increase the risk for severe COVID illness or that, in the opinion of the Investigator, is at a stage where it might interfere with study conduct or completion.
* Known liver disease or fatty liver.
* Positive test for chronic active Hepatitis B surface antigen, Hepatitis B core antibody, Hepatitis C antibody, or human immunodeficiency virus (HIV) antibody from blood work collected at screening visit.
* Bleeding disorder, or receipt of anticoagulants in the 3 weeks preceding inclusion, contraindicating intramuscular vaccination based on Investigator's judgment.
* Receipt of immunoglobulins, blood or blood-derived products in the past 3 months.
* Prior administration of a coronavirus vaccine (SARS-CoV-2, SARS-CoV, Middle East Respiratory Syndrome coronavirus \[MERS-CoV\]).
* Receipt of any vaccine in the 30 days preceding the first study vaccination or planned receipt of any vaccine in the 30 days following the last study vaccination except for influenza vaccination, which might be received at least 2 weeks before and a minimum of 2 weeks after study vaccines.
* Receipt of any therapy known to have in-vitro antiviral activity against SARS-CoV-2 within 72 hours prior to the first blood draw or planned use of such therapy 72 hours prior to study immunogenicity blood draws at Day 22 and Day 36.
* Residence in a nursing home or long-term care facility.
* Health care workers providing direct patient care for COVID-19 participants.

The above information was not intended to contain all considerations relevant to a participant's potential participation in a clinical trial.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Sanofi Pasteur, a Sanofi Company

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Clinical Sciences & Operations

Role: STUDY_DIRECTOR

Sanofi

Locations

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Investigational Site Number :8400003

Rolling Hills Estates, California, United States

Site Status

Investigational Site Number :8400002

Hollywood, Florida, United States

Site Status

Investigational Site Number :8400006

Miami, Florida, United States

Site Status

Investigational Site Number :8400017

Iowa City, Iowa, United States

Site Status

Investigational Site Number :8400007

Kansas City, Missouri, United States

Site Status

Investigational Site Number :8400008

Omaha, Nebraska, United States

Site Status

Investigational Site Number :8400001

Rochester, New York, United States

Site Status

Investigational Site Number :8400010

Philadelphia, Pennsylvania, United States

Site Status

Investigational Site Number :8400004

North Charleston, South Carolina, United States

Site Status

Investigational Site Number :8400015

Knoxville, Tennessee, United States

Site Status

Investigational Site Number :8400009

Houston, Texas, United States

Site Status

Investigational Site Number :8400005

Salt Lake City, Utah, United States

Site Status

Investigational Site Number :0360003

Morayfield, Queensland, Australia

Site Status

Investigational Site Number :0360005

South Brisbane, Queensland, Australia

Site Status

Investigational Site Number :0360001

Melbourne, Victoria, Australia

Site Status

Investigational Site Number :0360002

Nedlands, Western Australia, Australia

Site Status

Investigational Site Number :0760001

Salvador, Estado de Bahia, Brazil

Site Status

Investigational Site Number :0760004

Campo Grande, Mato Grosso do Sul, Brazil

Site Status

Investigational Site Number :0760003

Belo Horizonte, Minas Gerais, Brazil

Site Status

Investigational Site Number :3400002

Barrio del Centro, , Honduras

Site Status

Investigational Site Number :3400001

San Pedro Sula, , Honduras

Site Status

Countries

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United States Australia Brazil Honduras

Provided Documents

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Document Type: Study Protocol

View Document

Document Type: Statistical Analysis Plan

View Document

Related Links

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Other Identifiers

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U1111-1251-5486

Identifier Type: REGISTRY

Identifier Source: secondary_id

VAW00001

Identifier Type: -

Identifier Source: org_study_id

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