Study of Recombinant Protein Vaccine Formulations Against COVID-19 in Healthy Adults 18 Years of Age and Older

NCT ID: NCT04537208

Last Updated: 2025-09-17

Study Results

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

441 participants

Study Classification

INTERVENTIONAL

Study Start Date

2020-09-03

Study Completion Date

2021-11-19

Brief Summary

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The primary objectives of the study were:

* To describe the neutralizing antibody profile at Day 1, Day 22, and Day 36 of each study intervention group.
* To describe the safety profile of all participants in each age group and each study intervention group up to 12 months post-last injection.

The secondary objectives of the study are:

* To describe binding antibody profile at Day 1, Day 22, Day 36, Day 181 (Cohort 1) or Day 202 (Cohort 2), and Day 366 (Cohort 1) or Day 387 (Cohort 2) of each study intervention group.
* To describe the neutralizing antibody profile at Day 181 (Cohort 1) or Day 202 (Cohort 2) and at Day 366 (Cohort 1) and Day 387 (Cohort 2) of each study intervention group.
* To describe the occurrence of virologically-confirmed coronavirus disease (COVID-19)-like illness and serologically-confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.
* To evaluate the correlation / association between antibody responses to SARS-CoV-2 Recombinant Protein and the risk of virologically-confirmed COVID-19-like illness and/or serologically-confirmed SARS-CoV-2 infection.

Detailed Description

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The duration of each participant's participation in the study was approximately 365 days (Cohort 1) and 386 days (Cohort 2) post last injection.

Conditions

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COVID-19 (Healthy Volunteers)

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

This was a parallel group prevention study. Participants from 2 age groups (adults 18 through 49 years of age and adults 50 years of age and older) received either 1 injection (Cohort 1) or 2 injections (Cohort 2) of study vaccine or placebo control.

As a precautionary step, a sentinel safety cohort of 6 participants (younger adults only) within each dosing group from Cohort 1 was enrolled. An early safety data review was performed, including evaluation of safety data and laboratory measures to Day 9. Upon acceptable safety demonstrated from unblinded data review by limited members of the Sponsor Study Team, the remaining participants were enrolled simultaneously.
Primary Study Purpose

PREVENTION

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors
Participants, outcome assessors, Investigators, laboratory personnel, and the majority of Sponsor study staff will be blinded to vaccine group assignment; injection schedule will be unblinded and those preparing/administering the study interventions will be unblinded.

Study Groups

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Cohort 1: Group 1: SARS-CoV-2 vaccine LD + AF03

Participants received a single intramuscular (IM) injection of SARS-CoV2 vaccine low-dose (LD) formulation along with adjuvant AF03 on Day 1.

Group Type EXPERIMENTAL

CoV2 preS dTM-AF03 (low-dose)

Intervention Type BIOLOGICAL

Pharmaceutical form: liquid; route of administration: intramuscular injection

Cohort 1: Group 2: SARS-CoV-2 vaccine LD + AS03

Participants received a single IM injection of SARS-CoV2 vaccine LD formulation along with adjuvant AS03 on Day 1.

Group Type EXPERIMENTAL

CoV2 preS dTM-AS03 (low-dose)

Intervention Type BIOLOGICAL

Pharmaceutical form: liquid; route of administration: intramuscular injection

Cohort 1: Group 3: SARS-CoV-2 vaccine HD + AF03

Participants received a single IM injection of SARS-CoV2 vaccine high-dose (HD) formulation along with adjuvant AF03 on Day 1.

Group Type EXPERIMENTAL

CoV2 preS dTM-AF03 (high-dose)

Intervention Type BIOLOGICAL

Pharmaceutical form: liquid; route of administration: intramuscular injection

Cohort 1: Group 4: SARS-CoV-2 vaccine HD + AS03

Participants received a single IM injection of SARS-CoV2 vaccine HD formulation along with adjuvant AS03 on Day 1.

Group Type EXPERIMENTAL

CoV2 preS dTM-AS03 (high-dose)

Intervention Type BIOLOGICAL

Pharmaceutical form: liquid; route of administration: intramuscular injection

Cohort 1: Group 5: Placebo

Participants received an IM injection of placebo matching to SARS-CoV2 vaccine on Day 1.

Group Type PLACEBO_COMPARATOR

Placebo (0.9% normal saline)

Intervention Type BIOLOGICAL

Pharmaceutical form: liquid; route of administration: intramuscular injection

Cohort 2: Group 6: SARS-CoV-2 vaccine LD + AF03

Participants received IM injection of SARS-CoV2 vaccine LD formulation along with adjuvant AF03 on Day 1 and Day 22, respectively.

Group Type EXPERIMENTAL

CoV2 preS dTM-AF03 (low-dose)

Intervention Type BIOLOGICAL

Pharmaceutical form: liquid; route of administration: intramuscular injection

Cohort 2: Group 7: SARS-CoV-2 vaccine LD + AS03

Participants received IM injection of SARS-CoV2 vaccine LD formulation along with adjuvant AS03 on Day 1 and Day 22, respectively.

Group Type EXPERIMENTAL

CoV2 preS dTM-AS03 (low-dose)

Intervention Type BIOLOGICAL

Pharmaceutical form: liquid; route of administration: intramuscular injection

Cohort 2: Group 8: SARS-CoV-2 vaccine HD + AF03

Participants received IM injection of SARS-CoV2 vaccine HD formulation along with adjuvant AF03 on Day 1 and Day 22, respectively.

Group Type EXPERIMENTAL

CoV2 preS dTM-AF03 (high-dose)

Intervention Type BIOLOGICAL

Pharmaceutical form: liquid; route of administration: intramuscular injection

Cohort 2: Group 9: SARS-CoV-2 vaccine HD + AS03

Participants received IM injection of SARS-CoV2 vaccine HD formulation along with adjuvant AS03 on Day 1 and Day 22, respectively.

Group Type EXPERIMENTAL

CoV2 preS dTM-AS03 (high-dose)

Intervention Type BIOLOGICAL

Pharmaceutical form: liquid; route of administration: intramuscular injection

Cohort 2: Group 10: SARS-CoV-2 vaccine HD

Participants received a single IM injection of SARS-CoV2 vaccine HD formulation without adjuvant on Day 1 and Day 22, respectively.

Group Type EXPERIMENTAL

CoV2 preS dTM (high-dose) without adjuvant

Intervention Type BIOLOGICAL

Pharmaceutical form: liquid; route of administration: intramuscular injection

Cohort 2: Group 11: Placebo

Participants received an IM injection of placebo matching to SARS-CoV2 on Day 1 and Day 22, respectively.

Group Type PLACEBO_COMPARATOR

Placebo (0.9% normal saline)

Intervention Type BIOLOGICAL

Pharmaceutical form: liquid; route of administration: intramuscular injection

Interventions

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CoV2 preS dTM-AF03 (low-dose)

Pharmaceutical form: liquid; route of administration: intramuscular injection

Intervention Type BIOLOGICAL

CoV2 preS dTM-AF03 (high-dose)

Pharmaceutical form: liquid; route of administration: intramuscular injection

Intervention Type BIOLOGICAL

CoV2 preS dTM-AS03 (low-dose)

Pharmaceutical form: liquid; route of administration: intramuscular injection

Intervention Type BIOLOGICAL

CoV2 preS dTM-AS03 (high-dose)

Pharmaceutical form: liquid; route of administration: intramuscular injection

Intervention Type BIOLOGICAL

CoV2 preS dTM (high-dose) without adjuvant

Pharmaceutical form: liquid; route of administration: intramuscular injection

Intervention Type BIOLOGICAL

Placebo (0.9% normal saline)

Pharmaceutical form: liquid; route of administration: intramuscular injection

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* Aged 18 years of age or older on the day of inclusion.
* Informed consent form had been signed and dated.
* Able to attend all scheduled visits and complied with all study procedures.

Exclusion Criteria

* Participant was pregnant, or lactating, or of childbearing potential and not used an effective method of contraception or abstinence from at least 4 weeks prior to the first vaccination until at least 12 weeks after the last vaccination. To be considered of non-childbearing potential, a female was post-menopausal for at least 1 year or surgically sterile.
* Receipt of any vaccine in the 30 days preceding the first study vaccination or planned receipt of any vaccine in the 30 days following the last study vaccination except for influenza vaccination, which might be received at least 2 weeks before and a minimum of 2 weeks after study vaccines.
* Prior administration of a coronavirus vaccine SARS-CoV-2, SARS-CoV, Middle East Respiratory Syndrome).
* Known or suspected congenital or acquired immunodeficiency; or receipt of immunosuppressive therapy, such as anti-cancer chemotherapy or radiation therapy, within the preceding 6 months; or long-term systemic corticosteroid therapy (prednisone or equivalent for more than 2 consecutive weeks within the past 3 months).
* History of SARS-CoV-2 infection, confirmed either clinically, serologically, or microbiologically
* Chronic illness or condition considered to potentially increase the risk for severe COVID illness or that, in the opinion of the Investigator, was at a stage where it might interfere with study conduct or completion.
* Receipt of any therapy known to had in-vitro antiviral activity against SARS-CoV-2 within 72 hours prior to the first blood drew.
* Health care workers provided direct participant care for COVID-19 participants.

The above information was not intended to contain all considerations relevant to a participant's potential participation in a clinical trial.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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GlaxoSmithKline

INDUSTRY

Sponsor Role collaborator

Sanofi Pasteur, a Sanofi Company

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Clinical Sciences & Operations

Role: STUDY_DIRECTOR

Sanofi Pasteur, a Sanofi Company

Locations

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Investigational Site Number 8400004

Birmingham, Alabama, United States

Site Status

Investigational Site Number 8400012

Rolling Hills Estates, California, United States

Site Status

Investigational Site Number 8400011

Hollywood, Florida, United States

Site Status

Investigational Site Number 8400019

Melbourne, Florida, United States

Site Status

Investigational Site Number 8400016

Boston, Massachusetts, United States

Site Status

Investigational Site Number 8400002

Omaha, Nebraska, United States

Site Status

Investigational Site Number 8400001

Rochester, New York, United States

Site Status

Investigational Site Number 8400007

Rochester, New York, United States

Site Status

Investigational Site Number 8400008

Cleveland, Ohio, United States

Site Status

Investigational Site Number 8400003

Philadelphia, Pennsylvania, United States

Site Status

Investigational Site Number 8400014

Mt. Pleasant, South Carolina, United States

Site Status

Countries

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United States

References

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De Rosa SC, Cohen KW, Bonaparte M, Fu B, Garg S, Gerard C, Goepfert PA, Huang Y, Larocque D, McElrath MJ, Morris D, Van der Most R, de Bruyn G, Pagnon A. Whole-blood cytokine secretion assay as a high-throughput alternative for assessing the cell-mediated immunity profile after two doses of an adjuvanted SARS-CoV-2 recombinant protein vaccine candidate. Clin Transl Immunology. 2022 Jan 11;11(1):e1360. doi: 10.1002/cti2.1360. eCollection 2022.

Reference Type DERIVED
PMID: 35035955 (View on PubMed)

Goepfert PA, Fu B, Chabanon AL, Bonaparte MI, Davis MG, Essink BJ, Frank I, Haney O, Janosczyk H, Keefer MC, Koutsoukos M, Kimmel MA, Masotti R, Savarino SJ, Schuerman L, Schwartz H, Sher LD, Smith J, Tavares-Da-Silva F, Gurunathan S, DiazGranados CA, de Bruyn G. Safety and immunogenicity of SARS-CoV-2 recombinant protein vaccine formulations in healthy adults: interim results of a randomised, placebo-controlled, phase 1-2, dose-ranging study. Lancet Infect Dis. 2021 Sep;21(9):1257-1270. doi: 10.1016/S1473-3099(21)00147-X. Epub 2021 Apr 19.

Reference Type DERIVED
PMID: 33887209 (View on PubMed)

Provided Documents

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Document Type: Study Protocol

View Document

Document Type: Statistical Analysis Plan

View Document

Related Links

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Other Identifiers

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U1111-1250-4757

Identifier Type: OTHER

Identifier Source: secondary_id

VAT00001

Identifier Type: -

Identifier Source: org_study_id

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