Relationship Between Neutrophil Function and Sepsis in Adults and Children With Severe Thermal Injuries

NCT ID: NCT04693442

Last Updated: 2021-01-05

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

245 participants

Study Classification

OBSERVATIONAL

Study Start Date

2016-11-18

Study Completion Date

2022-01-31

Brief Summary

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The study aims to validate neutrophil function, immature granulocyte and plasma free DNA levels as predictive diagnostic biomarkers of sepsis in burn patients. Inclusion criteria includes healthy volunteers, patients aged 5-15 years old with burns affecting 20% or less Total Body Surface Area (TBSA) and patients aged 16 or above with burns affecting \>15% TBSA. The study involves taking blood, tissue and urine samples.

Detailed Description

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The initial cohort for SIFTI-2 will be generated from patients attending the Birmingham Burns Centre at Queen Elizabeth Hospital Birmingham (QEHB) and Birmingham Children's Hospital (BCH). The cohort will be comprised of patients with moderate and severe burns (injury to more than 15% of the body surface area \[TBSA\] in adults and 20% TBSA in children). The investigators currently treat between 30-40 patients in this category each year. In order to thoroughly characterise the longitudinal neutrophil response to burn injury and to investigate its potential relationship with outcome post-burn, the ivestigators will measure the genomic, phenotypic and functional response of peripheral blood neutrophils across time in burn-injured patients.

Conditions

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Burns

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Burn patients

with the condition

taking of blood, urine and blood samples and objective scar assessment

Intervention Type OTHER

blood, urine and skin tissue sampling \& scar assessment questionnaires

Control group

without the condition (blood sampling only for adults) and children undergoing general anaesthetic procedures that involve skin resections (blood sampling and excised skin)

No interventions assigned to this group

Interventions

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taking of blood, urine and blood samples and objective scar assessment

blood, urine and skin tissue sampling \& scar assessment questionnaires

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Patients aged 1-15 years admitted with a ≥20%TBSA
* Patients aged 16 and over admitted with a ≥15% TBSA
* Patients presented within 24 hours of thermal injury

Exclusion Criteria

* Associated multiple injuries with Injury severity score \> 25
* Decision not to treat made on admission due to the severity of the injury
* Patients with chemical and deep electrical burns
* Premorbid conditions
* Active Malignancy
* Patients receiving glucocorticoid treatment
* Multiple limb amputations
* Patients with known long term infections (i.e., Hepatitis B \& C, Human Immune Deficiency \[HIV\])
Minimum Eligible Age

1 Year

Maximum Eligible Age

99 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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The Scar Free Foundation

UNKNOWN

Sponsor Role collaborator

University of Birmingham

OTHER

Sponsor Role collaborator

University Hospital Birmingham NHS Foundation Trust

OTHER

Sponsor Role lead

Responsible Party

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Naiem Moiemen

Burns and Plastics Consultant

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Naiem Moiemen, Surgeon

Role: PRINCIPAL_INVESTIGATOR

The Scar Free Foundation

Locations

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University Hospitals Birmingham NHS Foundation Trust

Birmingham, , United Kingdom

Site Status RECRUITING

Countries

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United Kingdom

Central Contacts

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Amy Bamford, Registered Nurse

Role: CONTACT

01213718226 ext. 18226

Minnie Ventura, MSc

Role: CONTACT

01213718220 ext. 18220

Facility Contacts

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Minnie Ventura, MSc

Role: primary

01213718220 ext. 18220

Amy Bamford, RN

Role: backup

01213718226 ext. 18226

References

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Farina JA Jr, Rosique MJ, Rosique RG. Curbing inflammation in burn patients. Int J Inflam. 2013;2013:715645. doi: 10.1155/2013/715645. Epub 2013 May 20.

Reference Type BACKGROUND
PMID: 23762773 (View on PubMed)

Xiao W, Mindrinos MN, Seok J, Cuschieri J, Cuenca AG, Gao H, Hayden DL, Hennessy L, Moore EE, Minei JP, Bankey PE, Johnson JL, Sperry J, Nathens AB, Billiar TR, West MA, Brownstein BH, Mason PH, Baker HV, Finnerty CC, Jeschke MG, Lopez MC, Klein MB, Gamelli RL, Gibran NS, Arnoldo B, Xu W, Zhang Y, Calvano SE, McDonald-Smith GP, Schoenfeld DA, Storey JD, Cobb JP, Warren HS, Moldawer LL, Herndon DN, Lowry SF, Maier RV, Davis RW, Tompkins RG; Inflammation and Host Response to Injury Large-Scale Collaborative Research Program. A genomic storm in critically injured humans. J Exp Med. 2011 Dec 19;208(13):2581-90. doi: 10.1084/jem.20111354. Epub 2011 Nov 21.

Reference Type BACKGROUND
PMID: 22110166 (View on PubMed)

Jackson PC, Hardwicke J, Bamford A, Nightingale P, Wilson Y, Papini R, Moiemen N. Revised estimates of mortality from the Birmingham Burn Centre, 2001-2010: a continuing analysis over 65 years. Ann Surg. 2014 May;259(5):979-84. doi: 10.1097/SLA.0b013e31829160ca.

Reference Type BACKGROUND
PMID: 23598383 (View on PubMed)

Mann EA, Baun MM, Meininger JC, Wade CE. Comparison of mortality associated with sepsis in the burn, trauma, and general intensive care unit patient: a systematic review of the literature. Shock. 2012 Jan;37(1):4-16. doi: 10.1097/SHK.0b013e318237d6bf.

Reference Type BACKGROUND
PMID: 21941222 (View on PubMed)

Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D, Cohen J, Opal SM, Vincent JL, Ramsay G; SCCM/ESICM/ACCP/ATS/SIS. 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Crit Care Med. 2003 Apr;31(4):1250-6. doi: 10.1097/01.CCM.0000050454.01978.3B.

Reference Type BACKGROUND
PMID: 12682500 (View on PubMed)

Brinkmann V, Reichard U, Goosmann C, Fauler B, Uhlemann Y, Weiss DS, Weinrauch Y, Zychlinsky A. Neutrophil extracellular traps kill bacteria. Science. 2004 Mar 5;303(5663):1532-5. doi: 10.1126/science.1092385.

Reference Type BACKGROUND
PMID: 15001782 (View on PubMed)

Mocsai A. Diverse novel functions of neutrophils in immunity, inflammation, and beyond. J Exp Med. 2013 Jul 1;210(7):1283-99. doi: 10.1084/jem.20122220.

Reference Type BACKGROUND
PMID: 23825232 (View on PubMed)

Butler KL, Ambravaneswaran V, Agrawal N, Bilodeau M, Toner M, Tompkins RG, Fagan S, Irimia D. Burn injury reduces neutrophil directional migration speed in microfluidic devices. PLoS One. 2010 Jul 30;5(7):e11921. doi: 10.1371/journal.pone.0011921.

Reference Type BACKGROUND
PMID: 20689600 (View on PubMed)

Arturson G. Neutrophil granulocyte functions in severely burned patients. Burns Incl Therm Inj. 1985 Jun;11(5):309-19. doi: 10.1016/0305-4179(85)90093-2.

Reference Type BACKGROUND
PMID: 4027746 (View on PubMed)

Bjerknes R, Vindenes H, Laerum OD. Altered neutrophil functions in patients with large burns. Blood Cells. 1990;16(1):127-41; discussion 142-3.

Reference Type BACKGROUND
PMID: 2190644 (View on PubMed)

Jones CN, Moore M, Dimisko L, Alexander A, Ibrahim A, Hassell BA, Warren HS, Tompkins RG, Fagan SP, Irimia D. Spontaneous neutrophil migration patterns during sepsis after major burns. PLoS One. 2014 Dec 9;9(12):e114509. doi: 10.1371/journal.pone.0114509. eCollection 2014.

Reference Type BACKGROUND
PMID: 25489947 (View on PubMed)

The World Health Organisation. Burns [updated April 2014; cited 2015 06.10.2015]. Available from: http://www.who.int/mediacentre/factsheets/fs365/en/.

Reference Type BACKGROUND

Campisi J. Aging, cellular senescence, and cancer. Annu Rev Physiol. 2013;75:685-705. doi: 10.1146/annurev-physiol-030212-183653. Epub 2012 Nov 8.

Reference Type BACKGROUND
PMID: 23140366 (View on PubMed)

Tullie S, Asiri A, Acharjee A, Moiemen NS, Lord JM, Harrison P, Hazeldine J. Day One Cell-Free DNA Levels as an Objective Prognostic Marker of Mortality in Major Burns Patients. Cells. 2025 Jun 1;14(11):821. doi: 10.3390/cells14110821.

Reference Type DERIVED
PMID: 40497997 (View on PubMed)

Hazeldine J, McGee KC, Al-Tarrah K, Hassouna T, Patel K, Imran R, Bishop JRB, Bamford A, Barnes D, Wilson Y, Harrison P, Lord JM, Moiemen NS. Multicentre, longitudinal, observational cohort study to examine the relationship between neutrophil function and sepsis in adults and children with severe thermal injuries: a protocol for the Scientific Investigation of the Biological Pathways Following Thermal Injury-2 (SIFTI-2) study. BMJ Open. 2021 Oct 22;11(10):e052035. doi: 10.1136/bmjopen-2021-052035.

Reference Type DERIVED
PMID: 34686556 (View on PubMed)

Other Identifiers

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IRAS Project ID 200366

Identifier Type: OTHER

Identifier Source: secondary_id

RRK5814

Identifier Type: -

Identifier Source: org_study_id

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