L-arginine and Asymmetric Dimethylarginine (ADMA) and Lifestyle Protocols

NCT ID: NCT04406402

Last Updated: 2020-06-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

243 participants

Study Classification

INTERVENTIONAL

Study Start Date

2006-12-05

Study Completion Date

2008-12-10

Brief Summary

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Low serum L-arginine (Arg) and high asymmetric dimethylarginine (ADMA) can predict microvascular complications in type 2 diabetes (T2DM). The investigators examined whether or not Arg and ADMA are affected by dietary factors or lifestyle modification in overweight/ obese and T2DM subjects.

Detailed Description

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The investigators tested the effects on serum Arg and ADMA of a) a single load of ingested dextrose, protein, fat or alcohol, each consumed within 10 minutes; b) a single episode of physical exercise; c) a 12 weeks lifestyle modification program comprised of a standard dietary and physical activity counseling in obese or T2DM subjects on circulating Arg and ADMA (n=43)

Conditions

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Diabetes Mellitus, Type 2 Obesity Arginine Asymmetric Dimethylarginine

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SINGLE_GROUP

Primary Study Purpose

OTHER

Blinding Strategy

SINGLE

Outcome Assessors

Study Groups

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Acute glucose tolerance test

A standard oral glucose tolerance test (75 grams of dextrose in 180 cc of water)

Group Type EXPERIMENTAL

Acute dietary protocols and lifestyle modification

Intervention Type BEHAVIORAL

The investigators tested the effects on serum Arg and ADMA of a) a single load of ingested dextrose, protein, fat or alcohol, each consumed within 10 minutes; b) a single episode of physical exercise; c) a 12 weeks lifestyle modification program comprised of a standard dietary and physical activity counseling in obese or T2DM subjects on circulating Arg and ADMA (n=43).

Acute protein load test

A protein-rich, vanilla-flavored powder (Pro-gym, Telpharma, Is

Group Type EXPERIMENTAL

Acute dietary protocols and lifestyle modification

Intervention Type BEHAVIORAL

The investigators tested the effects on serum Arg and ADMA of a) a single load of ingested dextrose, protein, fat or alcohol, each consumed within 10 minutes; b) a single episode of physical exercise; c) a 12 weeks lifestyle modification program comprised of a standard dietary and physical activity counseling in obese or T2DM subjects on circulating Arg and ADMA (n=43).

Acute fat load test

a 100-gram portion of sweet cream containing 300 Kacls, of which 94% of the ingested calories were fat

Group Type EXPERIMENTAL

Acute dietary protocols and lifestyle modification

Intervention Type BEHAVIORAL

The investigators tested the effects on serum Arg and ADMA of a) a single load of ingested dextrose, protein, fat or alcohol, each consumed within 10 minutes; b) a single episode of physical exercise; c) a 12 weeks lifestyle modification program comprised of a standard dietary and physical activity counseling in obese or T2DM subjects on circulating Arg and ADMA (n=43).

Acute alcohol load test

Vodka (100 cc, 40% alcohol)

Group Type EXPERIMENTAL

Acute dietary protocols and lifestyle modification

Intervention Type BEHAVIORAL

The investigators tested the effects on serum Arg and ADMA of a) a single load of ingested dextrose, protein, fat or alcohol, each consumed within 10 minutes; b) a single episode of physical exercise; c) a 12 weeks lifestyle modification program comprised of a standard dietary and physical activity counseling in obese or T2DM subjects on circulating Arg and ADMA (n=43).

Acute exercise

30 minutes of supervised graded walking on a treadmill according to each subject's individual ability. A goal heart rate was calculated as 70% of the age-adjusted maximal allowable heart rate.

Group Type EXPERIMENTAL

Acute dietary protocols and lifestyle modification

Intervention Type BEHAVIORAL

The investigators tested the effects on serum Arg and ADMA of a) a single load of ingested dextrose, protein, fat or alcohol, each consumed within 10 minutes; b) a single episode of physical exercise; c) a 12 weeks lifestyle modification program comprised of a standard dietary and physical activity counseling in obese or T2DM subjects on circulating Arg and ADMA (n=43).

Lifestyle modification program - 12 weeks

12 weeks of weight-loss dietary program constructed according to the guidelines of the American Diabetes Association. Based on weight, gender, and age, daily dietary allowance varied at 1200-1800 Kcal, 50% carbohydrates, 20% protein, and 30% fat. Participants were also asked to engage in moderate physical activity comprised of a 40-minute walk three times a week. A weekly clinic visit alternating with a weekly telephone contact was also required.

Group Type EXPERIMENTAL

Acute dietary protocols and lifestyle modification

Intervention Type BEHAVIORAL

The investigators tested the effects on serum Arg and ADMA of a) a single load of ingested dextrose, protein, fat or alcohol, each consumed within 10 minutes; b) a single episode of physical exercise; c) a 12 weeks lifestyle modification program comprised of a standard dietary and physical activity counseling in obese or T2DM subjects on circulating Arg and ADMA (n=43).

Interventions

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Acute dietary protocols and lifestyle modification

The investigators tested the effects on serum Arg and ADMA of a) a single load of ingested dextrose, protein, fat or alcohol, each consumed within 10 minutes; b) a single episode of physical exercise; c) a 12 weeks lifestyle modification program comprised of a standard dietary and physical activity counseling in obese or T2DM subjects on circulating Arg and ADMA (n=43).

Intervention Type BEHAVIORAL

Eligibility Criteria

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Inclusion Criteria

* obese and/ or T2DM subjects

Exclusion Criteria

* type 1 diabetes
* insulin-treated T2DM
* active coronary disease (recent \[≤ 6months\] myocardial infarction, unstable angina, recent coronary catheterization, or coronary bypass grafting
* individuals with significant impairment in liver enzymes (≥ 2.5 the upper limit of the normal range)
* serum creatinine higher than 1.5mg%
* known malabsorption, alcoholism or illicit use of drugs
* abnormal thyroid function tests
* concomitant or recent use of glucocorticoids
* uncertain mental state (to avoid invalid informed consent or lack of compliance).
Minimum Eligible Age

40 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Wolfson Medical Center

OTHER_GOV

Sponsor Role collaborator

Tel-Aviv Sourasky Medical Center

OTHER_GOV

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Naftali Stern, MD

Role: PRINCIPAL_INVESTIGATOR

Tel-Aviv Sourasky Medical Center

Locations

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Diabetes Unit, E. Wolfson Medical Center

Holon, , Israel

Site Status

Countries

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Israel

References

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Buch A, Ganz T, Wainstein J, Gilad S, Limor R, Shefer G, Boaz M, Stern N. Alcohol Drinks Induce Acute Lowering in Circulating l-Arginine in Obese and Type 2 Diabetic Subjects. J Med Food. 2022 Jun;25(6):675-682. doi: 10.1089/jmf.2021.0045.

Reference Type DERIVED
PMID: 35708634 (View on PubMed)

Other Identifiers

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03

Identifier Type: -

Identifier Source: org_study_id

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