Neutrophil Phenotypic Profiling and Acute Lung Injury in Patients After Cardiopulmonary Bypass (CPB)

NCT ID: NCT04296071

Last Updated: 2024-08-23

Basic Information

• Recruitment Status: RECRUITING
• Total Enrollment: 56 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2022-10-14
• Study Completion Date: 2026-12-31

Brief Summary

Acute lung injury (ALI) following cardiopulmonary bypass (CPB) is a serious complication, often prolonging the length of stay in ICU and potentially dealing to mortality. The objective of this study is to assess the mechanism of CPB-mediated acute lung injury in pediatric patients.

Detailed Description

Acute lung injury (ALI) is frequently associated with the use of extracorporeal circulation during cardiopulmonary bypass (CPB) surgery and develops postoperatively in 2-3% of cardiac surgical patients. Histological evidence shows that CPB increases pulmonary vascular permeability and extravascular lung water content while diminishing pulmonary compliance. Furthermore, some patients can develop acute respiratory distress syndrome, which has a mortality rate of 50-70%. Recruitment of intrapulmonary neutrophils is a characteristic of ALI following CPB. Blood contact with non-physiological surfaces, cooling and rewarming and mechanical shear stress activate neutrophils. The recruitment of activated neutrophils from blood vessels to local tissue involves a chain of well-coordinated events, including adhesion, tethering, rolling and crawling, followed by trans-endothelial and trans-epithelial migration. Activation of sequestered neutrophils causes the release of specific proteolytic enzymes and oxygen free radicals, which leads to increased alveolar-endothelial permeability and parenchymal damage. During CPB, the lungs are almost completely excluded from the systemic circulation, which causes the blood within them to be almost 'static'. Pulmonary tissue hypoxia and re-oxygenation combined with vascular ischemia and reperfusion induce the generation of chemokines, which contributes to subsequent injury by accumulating and entrapping activated neutrophils. The accumuled and entrapped activated neutrophils in the lungs and the subsequent release of toxic substances render the lungs highly susceptible to this damage. However, the mechanism that drives neutrophil migration to the lungs after CPB is not well studied. This study will delineate the mechanisms of neutrophil migration to the lung and subsequent lung injury after CPB.

Conditions

Lung Injury; Cardiopulmonary Bypass

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: PROSPECTIVE

Study Groups

Group 1

patients who tend to have longer CPB

Research study

Intervention Type: OTHER

Blood and tracheal aspirates will be collected

Group 2

Patients who have shorter CPB

Research study

Intervention Type: OTHER

Blood and tracheal aspirates will be collected

Interventions

Research study

Blood and tracheal aspirates will be collected

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Are < 12months of age
• Scheduled for cardiac surgical needing CPB
• Preoperative SpO2 > 90%

Exclusion Criteria

• Lack of parental (or legal guardian's) consent
• Preoperative SpO2 < 90%
• Preoperative oxygen therapy

• Maximum Eligible Age: 12 Months
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Sophia Koutsogiannaki

OTHER

Sponsor Role: lead

Responsible Party

Sophia Koutsogiannaki

Assistant Professor

Responsibility Role: SPONSOR_INVESTIGATOR

Locations

Boston Children's Hospital

Boston, Massachusetts, United States

Site Status: RECRUITING

Countries

United States

Central Contacts

Sophia Koutsogiannaki, PhD

Role: CONTACT

sophia.koutsogiannaki@childrens.harvard.edu

617-919-4725

Koichi Yuki, MD

Role: CONTACT

koichi.yuki@childrens.harvard.edu

617-355-6225

Facility Contacts

Rachel Bernier

Role: primary

Other Identifiers

IRB-P00034280

Identifier Type: -

Identifier Source: org_study_id