GPx Activity in Subjects With Aortic Stenosis Undergoing TAVR

NCT ID: NCT04268160

Last Updated: 2021-06-30

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

WITHDRAWN

Study Classification

OBSERVATIONAL

Study Start Date

2020-11-30

Study Completion Date

2021-06-15

Brief Summary

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The aim of this project is to investigate the association of glutathione peroxidase (GPx) and severe aortic stenosis (AS), as well as the impact of transcatheter aortic valve replacement (TAVR) on GPx activity post-procedure. The burden of oxidative stress will be determined by the measurement of GPx, superoxide dismutase (SOD) and lipoprotein A (Lp(a)). We hypothesize GPx activity is reduced in participants with severe AS vs control groups and GPx activity is to increase after TAVR is performed.

Detailed Description

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Conditions

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Glutathione Peroxidase Activity Aortic Stenosis Transcatheter Aortic Valve Replacement

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Patients with severe aortic stenosis undergoing TAVR

GPx activity levels will be measured on the day of TAVR procedure, the day of discharge, 1 month, and 6 months after the procedure

Blood draw to measure GPx activity

Intervention Type DIAGNOSTIC_TEST

Routine blood draw to measure levels of blood markers.

Blood draw to measure SOD activity

Intervention Type DIAGNOSTIC_TEST

Routine blood draw to measure levels of blood markers.

Blood draw to measure Lp(a) levels

Intervention Type DIAGNOSTIC_TEST

Routine blood draw to measure levels of blood markers.

Patients without aortic stenosis

GPx activity levels will be measured on day of recruitment

Blood draw to measure GPx activity

Intervention Type DIAGNOSTIC_TEST

Routine blood draw to measure levels of blood markers.

Blood draw to measure SOD activity

Intervention Type DIAGNOSTIC_TEST

Routine blood draw to measure levels of blood markers.

Blood draw to measure Lp(a) levels

Intervention Type DIAGNOSTIC_TEST

Routine blood draw to measure levels of blood markers.

Interventions

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Blood draw to measure GPx activity

Routine blood draw to measure levels of blood markers.

Intervention Type DIAGNOSTIC_TEST

Blood draw to measure SOD activity

Routine blood draw to measure levels of blood markers.

Intervention Type DIAGNOSTIC_TEST

Blood draw to measure Lp(a) levels

Routine blood draw to measure levels of blood markers.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Age \>18 years
* Patients with severe AS including participants with low flow, low gradient severe AS and paradoxical severe AS
* Patients meeting criteria for TAVR procedure

Exclusion Criteria

* Previous aortic valve replacement (surgical or TAVR)
* Participants undergoing chemotherapy
* End stage liver disease/cirrhosis (liver produces GSH)
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Florida

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Chinelo Udemgba, MD

Role: PRINCIPAL_INVESTIGATOR

University of Florida

Locations

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University of Florida

Jacksonville, Florida, United States

Site Status

Countries

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United States

References

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Redfors, B. A. (2017). Biomarkers in Aortic Stenosis: A systematic Review. Structural Heart. The Journal of the Heart Team, 1-13.

Reference Type BACKGROUND

Otto CM. Aortic stenosis and hyperlipidemia: establishing a cause-effect relationship. Am Heart J. 2004 May;147(5):761-3. doi: 10.1016/j.ahj.2003.12.014. No abstract available.

Reference Type BACKGROUND
PMID: 15131528 (View on PubMed)

Memet C, Gerede DM, Ozenci M, Akbulut IM, Acibuca A, Kilickap M, Erol C. Evaluation of the Role of Oxidative Stress in Degenerative Aortic Stenosis. J Heart Valve Dis. 2015 Jul;24(4):445-50.

Reference Type BACKGROUND
PMID: 26897814 (View on PubMed)

Nimse, S. B. (2015). Free radicals, natural antioxidants, and their reaction mechanism. Royal Society of Chemistry, 27986-28006.

Reference Type BACKGROUND

Blankenberg S, Rupprecht HJ, Bickel C, Torzewski M, Hafner G, Tiret L, Smieja M, Cambien F, Meyer J, Lackner KJ; AtheroGene Investigators. Glutathione peroxidase 1 activity and cardiovascular events in patients with coronary artery disease. N Engl J Med. 2003 Oct 23;349(17):1605-13. doi: 10.1056/NEJMoa030535.

Reference Type BACKGROUND
PMID: 14573732 (View on PubMed)

Bolzan AD, Bianchi MS, Bianchi NO. Superoxide dismutase, catalase and glutathione peroxidase activities in human blood: influence of sex, age and cigarette smoking. Clin Biochem. 1997 Aug;30(6):449-54. doi: 10.1016/s0009-9120(97)00047-7.

Reference Type BACKGROUND
PMID: 9316738 (View on PubMed)

Zheng KH, Tsimikas S, Pawade T, Kroon J, Jenkins WSA, Doris MK, White AC, Timmers NKLM, Hjortnaes J, Rogers MA, Aikawa E, Arsenault BJ, Witztum JL, Newby DE, Koschinsky ML, Fayad ZA, Stroes ESG, Boekholdt SM, Dweck MR. Lipoprotein(a) and Oxidized Phospholipids Promote Valve Calcification in Patients With Aortic Stenosis. J Am Coll Cardiol. 2019 May 7;73(17):2150-2162. doi: 10.1016/j.jacc.2019.01.070.

Reference Type BACKGROUND
PMID: 31047003 (View on PubMed)

Arsenault BJ, Boekholdt SM, Dube MP, Rheaume E, Wareham NJ, Khaw KT, Sandhu MS, Tardif JC. Lipoprotein(a) levels, genotype, and incident aortic valve stenosis: a prospective Mendelian randomization study and replication in a case-control cohort. Circ Cardiovasc Genet. 2014 Jun;7(3):304-10. doi: 10.1161/CIRCGENETICS.113.000400. Epub 2014 Apr 5.

Reference Type BACKGROUND
PMID: 24704946 (View on PubMed)

Mahmaljy H, Tawney A, Young M. Transcatheter Aortic Valve Replacement. 2023 Jul 24. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from http://www.ncbi.nlm.nih.gov/books/NBK431075/

Reference Type BACKGROUND
PMID: 28613729 (View on PubMed)

Other Identifiers

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IRB201902486

Identifier Type: -

Identifier Source: org_study_id

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