Treatment of Knee Osteoarthritis With Autologous Adipose-derived Mesenchymal Stem Cells

NCT ID: NCT04212728

Last Updated: 2023-03-28

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

60 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-12-29

Study Completion Date

2024-12-31

Brief Summary

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The purpose of this study is to explore the efficacy and safety of autologous adipose-derived mesenchymal stem cells (AMSCs) plus autologous platelet rich plasma (PRP) in the treatment of severe knee osteoarthritis.

Detailed Description

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This is a single centered, randomized, single blind phase II clinical study. Patients will be divided into two groups of case and control. Patients of case group will receive intra-articular injection of autologous AMSCs suspended in 3 ml autologous PRP for 3 times, patients of control group will receive intra-articular injection of 3 ml of autologous PRP for 3 times. The investigators designed this clinical study to evaluate therapeutic effects of AMSCs in patients with severe knee osteoarthritis.

Conditions

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Knee Osteoarthritis

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Outcome Assessors

Study Groups

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AMSCs plus PRP group

Three intra-articular injections in total and autologous adipose-derived mesenchymal stem cells (AMSCs) suspended in 3 ml autologous platelet rich plasma (PRP) for each injection. Time-points for intervention: 1) initial injection; 2) 3 months following initial injection; 3) 6 months following initial injection.

Group Type EXPERIMENTAL

Auotologous AMSCs plus autologous PRP

Intervention Type BIOLOGICAL

150 mL subcutaneous abdominal adipose tissue will be harvested via liposuction . AMSCs will be isolated and cultured from adipose tissue. Before injection, 30 mL of blood will be collected from each patient to produce 3ml PRP by cenrifugation.Cultured AMSCs will be collected and suspended by 3ml autologous PRP.

PRP group

Three intra-articular injections in total and 3 ml autologous platelet rich plasma (PRP) for each injection. Time-points for intervention: 1) initial injection; 2) 3 months following initial injection; 3) 6 months following initial injection.

Group Type ACTIVE_COMPARATOR

Auotologous PRP

Intervention Type BIOLOGICAL

30 mL of blood will be collected from each patient to produce 3ml PRP by cenrifugation.

Interventions

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Auotologous AMSCs plus autologous PRP

150 mL subcutaneous abdominal adipose tissue will be harvested via liposuction . AMSCs will be isolated and cultured from adipose tissue. Before injection, 30 mL of blood will be collected from each patient to produce 3ml PRP by cenrifugation.Cultured AMSCs will be collected and suspended by 3ml autologous PRP.

Intervention Type BIOLOGICAL

Auotologous PRP

30 mL of blood will be collected from each patient to produce 3ml PRP by cenrifugation.

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

1. Subject has Kellgren and Lawrence grade II-IV primary osteoarthritis as determined by X-ray.
2. Subject's pain score is 8-13 points (Lequesne's index).
3. Ages between 40-70 years.
4. Signed informed consent from the subject.

Exclusion Criteria

1. Subject infected with hepatitis B, hepatitis C, HIV, syphilis or HTLV
2. Subject not suitable for liposuction surgery.
3. Subject with hypersensitivity/allergy to anesthetic.
4. Subject's creatinine values higher than 1.6mg/dl.
5. Subject with body mass index, BMI over 30.
6. Subject's studied knee treated with intra-articular injection therapy within 6 months prior to screen.
7. Subject has undergone surgery on studied knee, including fracture surgery, arthroscopic surgery, meniscus repair surgery, or cruciate ligament reconstruction surgery.
8. Subject enrolled in any other cell therapy studies within the past 30 days.
9. Subject who the investigator considers inappropriate for the clinical trial due to any other reasons than those listed above.
10. Subject has a history of gouty arthritis, septic arthritis, rheumatoid arthritis and any other autoimmune arthritis of the knee joint.
11. Subject has had major medical problems in vital organs, such as; heart, liver, kidney, or lung.
Minimum Eligible Age

40 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Yantai Yuhuangding Hospital

OTHER

Sponsor Role lead

Responsible Party

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Peiwen Lian

Peiwen Lian Ph.D

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Peiwen Lian, PhD

Role: STUDY_DIRECTOR

Yantai Yuhuangding Hospital

Locations

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Yantai Yuhuangding Hospital

Yantai, Shandong, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Peiwen Lian, PhD

Role: CONTACT

+0086 5356691999 ext. 81511

Jian Chen, PhD

Role: CONTACT

+0086 5356691999 ext. 82708

Facility Contacts

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Peiwen Lian, PhD

Role: primary

+0086 5356691999 ext. 82708

Other Identifiers

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2019-003

Identifier Type: -

Identifier Source: org_study_id

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