Evaluation of Bioavailability and Metabolism of Diet Phenolic Compounds

NCT ID: NCT03614520

Last Updated: 2020-03-05

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

20 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-07-09

Study Completion Date

2019-02-27

Brief Summary

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This study aims at studying in depth the absorption and metabolism of phenolic compounds of olive oil, wine and beer. This study is divided into 2 sub-studies in order to evaluate each one of the objectives.

Detailed Description

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The study is divided in two sub-studies to explore each objective.

One the one hand, a group of people will drink olive oil, or wine, or both. This is done to see if combining these two drinks will improve the absorption and bioavailibility of phenolic compounds that they contain, promoting by synergy their antioxidant activity at a postprandial level. The main compounds studied are the Resveratrol (RSVT), the Hydroxytyrosol (HT), tyrosol (TIR) and their metabolits.

One the other hand, an group of people will drink 3 different beers ( with 3 different degrees of alcohol), or wine, in order to study the absorption of TIR in relation to the alcohol degree. It also aims at assessing if the gas contained in beer contributes to TIR absorption.

At different times after the administration of drinks, urine and blood samples will be collected.

Conditions

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Healthy

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

A 40 people group of healthy men and women. Two Cohorts group of 20 healthy volunteers of both genders.
Primary Study Purpose

HEALTH_SERVICES_RESEARCH

Blinding Strategy

NONE

The subjects, because of the wine taste that cannot be hidden, will know what they drink, except from the three beers (the three types will not be distinguishable).

Study Groups

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Sub-study A : olive oil, wine, both, or water (placebo).

After being selected, subjects will do 4 experimental sessions (each separated by 3 days minimum) in which ones they will drink olive oil, red wine, red wine and olive oil, or water (placebo). The order of the experimental sessions will be drawn.

Group Type EXPERIMENTAL

Administration of olive oil

Intervention Type OTHER

25 mL of extra virgin olive oil

Administration of red wine

Intervention Type OTHER

150 mL or Red Wine

Combination of red wine and olive oil

Intervention Type OTHER

150 mL of Red wine + 25 mL of Extra Virgin Olive oil will be administred at the same time

Water

Intervention Type OTHER

Mineral water will be given as placebo

Sub-study B : three types of beer, and wine

The subjects will do 4 experimental sessions (each separated by 3 days minimum) in wich ones they will drink a beer (250mL) or wine (150mL). The order of the experimental sessions will be drawn.

Group Type EXPERIMENTAL

Administration of red wine

Intervention Type OTHER

150 mL or Red Wine

Dark beer

Intervention Type OTHER

250 mL of IPA beer (alcohol 8.5% vol)

Light Beer

Intervention Type OTHER

250 mL of blonde ale beer (alcohol 4,5% vol)

Alcohol free Beer

Intervention Type OTHER

250 mL of alcohol free beer (alcohol 0.0% vol)

Interventions

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Administration of olive oil

25 mL of extra virgin olive oil

Intervention Type OTHER

Administration of red wine

150 mL or Red Wine

Intervention Type OTHER

Combination of red wine and olive oil

150 mL of Red wine + 25 mL of Extra Virgin Olive oil will be administred at the same time

Intervention Type OTHER

Water

Mineral water will be given as placebo

Intervention Type OTHER

Dark beer

250 mL of IPA beer (alcohol 8.5% vol)

Intervention Type OTHER

Light Beer

250 mL of blonde ale beer (alcohol 4,5% vol)

Intervention Type OTHER

Alcohol free Beer

250 mL of alcohol free beer (alcohol 0.0% vol)

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Men and women from 18 to 45 years old.
* Understand and accepting the procedures of the trial and sign an informed consent.
* Have a history and physical exams that show that there is no organic issue, and an analysis and ECG in the normal limits.
* Have an BMI between 18.5 and 30 kg/m2.
* caucasian race

Exclusion Criteria

* Smokers
* Persons with chronical disease
* Persons with BMI\>30 or \<18.5 kg/m2.
* Persons with history of multiple allergies or obvious intestinal, hepatic, renal issues or other problems that could suppose a deterioration of absorption, distribution or metabolism of polyphenols.
* Persons who take anti-oxidant products, including vitamins, herbal medication or dietetics complementation that could interfere in the study objectives.
* Persons with restrictive diet (including vegetarian diet).
* Persons with history of hypersensibility or intolerance to alcohol.
* Persons with a daily consumption of alcohol \>50g or who have consumed illegal drug in the month preceding the study.
* Persons who have participated in an other clinical trial the month preceding the study.
* Persons who have done a blood donation during the last 3 months before the beginning of the study (only appliable to the subjects of A sub-study).
* Persons who have a positive serology for B or C hepatitis or HIV.
* Pregnant or breastfeeding women, or any other situation prohibiting alcohol consumption.
* Persons who have consummed NSAIDs (especially acetylsalicylic acid) or antioxidants or vitamin complementation, during the 2 weeks preceding the beginning of the study.
* Illiterate persons
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Parc de Salut Mar

OTHER

Sponsor Role lead

Responsible Party

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Rafael de la Torre

Director of the Neurosciences Department in IMIM

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Rafael de la Torre Fornell, Dr

Role: PRINCIPAL_INVESTIGATOR

IMIM (Hospital del Mar Medical Research Institute)

Locations

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Consorci Parc de Salut Mar

Barcelona, , Spain

Site Status

Countries

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Spain

References

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Wu L, Sun D. Adherence to Mediterranean diet and risk of developing cognitive disorders: An updated systematic review and meta-analysis of prospective cohort studies. Sci Rep. 2017 Jan 23;7:41317. doi: 10.1038/srep41317.

Reference Type BACKGROUND
PMID: 28112268 (View on PubMed)

Rodriguez-Morato J, Robledo P, Tanner JA, Boronat A, Perez-Mana C, Oliver Chen CY, Tyndale RF, de la Torre R. CYP2D6 and CYP2A6 biotransform dietary tyrosol into hydroxytyrosol. Food Chem. 2017 Feb 15;217:716-725. doi: 10.1016/j.foodchem.2016.09.026. Epub 2016 Sep 7.

Reference Type BACKGROUND
PMID: 27664690 (View on PubMed)

Perez-Mana C, Farre M, Rodriguez-Morato J, Papaseit E, Pujadas M, Fito M, Robledo P, Covas MI, Cheynier V, Meudec E, Escudier JL, de la Torre R. Moderate consumption of wine, through both its phenolic compounds and alcohol content, promotes hydroxytyrosol endogenous generation in humans. A randomized controlled trial. Mol Nutr Food Res. 2015 Jun;59(6):1213-6. doi: 10.1002/mnfr.201400842. Epub 2015 Apr 27.

Reference Type BACKGROUND
PMID: 25712532 (View on PubMed)

Granados-Principal S, Quiles JL, Ramirez-Tortosa CL, Sanchez-Rovira P, Ramirez-Tortosa MC. Hydroxytyrosol: from laboratory investigations to future clinical trials. Nutr Rev. 2010 Apr;68(4):191-206. doi: 10.1111/j.1753-4887.2010.00278.x.

Reference Type BACKGROUND
PMID: 20416016 (View on PubMed)

Oliveras-Lopez MJ, Molina JJ, Mir MV, Rey EF, Martin F, de la Serrana HL. Extra virgin olive oil (EVOO) consumption and antioxidant status in healthy institutionalized elderly humans. Arch Gerontol Geriatr. 2013 Sep-Oct;57(2):234-42. doi: 10.1016/j.archger.2013.04.002. Epub 2013 May 1.

Reference Type BACKGROUND
PMID: 23642776 (View on PubMed)

Weinbrenner T, Fito M, de la Torre R, Saez GT, Rijken P, Tormos C, Coolen S, Albaladejo MF, Abanades S, Schroder H, Marrugat J, Covas MI. Olive oils high in phenolic compounds modulate oxidative/antioxidative status in men. J Nutr. 2004 Sep;134(9):2314-21. doi: 10.1093/jn/134.9.2314.

Reference Type BACKGROUND
PMID: 15333722 (View on PubMed)

Castaner O, Covas MI, Khymenets O, Nyyssonen K, Konstantinidou V, Zunft HF, de la Torre R, Munoz-Aguayo D, Vila J, Fito M. Protection of LDL from oxidation by olive oil polyphenols is associated with a downregulation of CD40-ligand expression and its downstream products in vivo in humans. Am J Clin Nutr. 2012 May;95(5):1238-44. doi: 10.3945/ajcn.111.029207. Epub 2012 Mar 21.

Reference Type BACKGROUND
PMID: 22440854 (View on PubMed)

Hernaez A, Fernandez-Castillejo S, Farras M, Catalan U, Subirana I, Montes R, Sola R, Munoz-Aguayo D, Gelabert-Gorgues A, Diaz-Gil O, Nyyssonen K, Zunft HJ, de la Torre R, Martin-Pelaez S, Pedret A, Remaley AT, Covas MI, Fito M. Olive oil polyphenols enhance high-density lipoprotein function in humans: a randomized controlled trial. Arterioscler Thromb Vasc Biol. 2014 Sep;34(9):2115-9. doi: 10.1161/ATVBAHA.114.303374. Epub 2014 Jul 24.

Reference Type BACKGROUND
PMID: 25060792 (View on PubMed)

Ruano J, Lopez-Miranda J, de la Torre R, Delgado-Lista J, Fernandez J, Caballero J, Covas MI, Jimenez Y, Perez-Martinez P, Marin C, Fuentes F, Perez-Jimenez F. Intake of phenol-rich virgin olive oil improves the postprandial prothrombotic profile in hypercholesterolemic patients. Am J Clin Nutr. 2007 Aug;86(2):341-6. doi: 10.1093/ajcn/86.2.341.

Reference Type BACKGROUND
PMID: 17684203 (View on PubMed)

Ruano J, Lopez-Miranda J, Fuentes F, Moreno JA, Bellido C, Perez-Martinez P, Lozano A, Gomez P, Jimenez Y, Perez Jimenez F. Phenolic content of virgin olive oil improves ischemic reactive hyperemia in hypercholesterolemic patients. J Am Coll Cardiol. 2005 Nov 15;46(10):1864-8. doi: 10.1016/j.jacc.2005.06.078. Epub 2005 Oct 24.

Reference Type BACKGROUND
PMID: 16286173 (View on PubMed)

De la Torre R, Corella D, Castaner O, Martinez-Gonzalez MA, Salas-Salvado J, Vila J, Estruch R, Sorli JV, Aros F, Fiol M, Ros E, Serra-Majem L, Pinto X, Gomez-Gracia E, Lapetra J, Ruiz-Canela M, Basora J, Asensio EM, Covas MI, Fito M. Protective effect of homovanillyl alcohol on cardiovascular disease and total mortality: virgin olive oil, wine, and catechol-methylation. Am J Clin Nutr. 2017 Jun;105(6):1297-1304. doi: 10.3945/ajcn.116.145813. Epub 2017 Apr 26.

Reference Type BACKGROUND
PMID: 28446500 (View on PubMed)

Other Identifiers

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IMIMFTCL/DOPET4

Identifier Type: -

Identifier Source: org_study_id

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