Interaction Between Non-typhoid Salmonella, Host Microbiota, and Immune System During Acute Infection and Remission

NCT ID: NCT03494101

Last Updated: 2018-07-03

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

40 participants

Study Classification

OBSERVATIONAL

Study Start Date

2018-06-15

Study Completion Date

2020-01-31

Brief Summary

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Stool and blood samples from patients with a non-typhoid Salmonella infection will be collected during an observation period of six months and analyzed for changes in the microbiota diversity and composition, mutation rates in the Salmonella strains and the specific immune response evoked by the infection. Findings are compared to healthy individuals and individuals with acute, infectious diarrhea caused by other microorganisms.

Detailed Description

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Infection processes of a non-typhoid Salmonella infection in humans are not well understood and so far, only little research has been conducted in this area. Findings from preclinical studies, using mouse models, attributed a fundamental role in infection control to the gut microbiota and the host immune system (antibody response). In mouse models a non-typhoid Salmonella infection provokes a pronounced antibody response and salmonella-inflicted gut inflammation alters the microbiota diversity and composition in the gut lumen. To date there is only scarce evidence on similar effects in humans.

During the study, longitudinal stool and blood samples will be collected from patients with a non-typhoid Salmonella infection at different study time points (2 weeks, 4 weeks and 6 months after positive Salmonella stool culture) and analyzed for changes in the microbiota, mutation rates in the Salmonella strains and the specific immune response evoked by the infection (e.g. anti-bodies). At each study time point clinical information will be investigated with a questionnaire to assess current symptoms, medication etc. Findings will be compared to healthy individuals and patients with acute, infectious diarrhea caused by other microorganisms than non-typhoid Salmonella.

Conditions

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Salmonella Infection Non-Typhoid

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Non-typhoid Salmonella infection

Patients with a non-typhoid Salmonella infection. Blood samples, stool samples and clinical information will be collected.

Blood samples

Intervention Type OTHER

Blood samples will be collected and analyzed at different study time points

Stool samples

Intervention Type OTHER

Stool samples will be collected and analyzed at different study time points

Clinical information

Intervention Type OTHER

Clinical information will be collected at different study time points using questionnaires

Acute, infectious diarrhea

Patients with acute, infectious diarrhea without non-typhoid Salmonella infection. Blood samples, stool samples and clinical information will be collected.

Blood samples

Intervention Type OTHER

Blood samples will be collected and analyzed at different study time points

Stool samples

Intervention Type OTHER

Stool samples will be collected and analyzed at different study time points

Clinical information

Intervention Type OTHER

Clinical information will be collected at different study time points using questionnaires

Healthy individuals

Healthy individuals with no symptoms of acute or chronic diarrhea. Blood samples, stool samples and clinical information will be collected.

Blood samples

Intervention Type OTHER

Blood samples will be collected and analyzed at different study time points

Stool samples

Intervention Type OTHER

Stool samples will be collected and analyzed at different study time points

Clinical information

Intervention Type OTHER

Clinical information will be collected at different study time points using questionnaires

Interventions

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Blood samples

Blood samples will be collected and analyzed at different study time points

Intervention Type OTHER

Stool samples

Stool samples will be collected and analyzed at different study time points

Intervention Type OTHER

Clinical information

Clinical information will be collected at different study time points using questionnaires

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Signed informed consent.
* Ability to understand and follow study procedures and understand informed consent
* Age 18-75 years.


* Acute diarrhea (≥3 bowel movements per day for ≤4 weeks)
* Stool cultures positive for non-typhoid Salmonella ≤4 weeks before inclusion


* Acute diarrhea (≥3 bowel movements per day for ≤4 weeks)
* Stool cultures negative for non-typhoid Salmonella infection within ≤ 4 weeks


• No symptoms of acute or chronic diarrhea (2 bowel movements per week to 2 per day)

Exclusion Criteria

* Current use of antibiotics
* Medication with immunosuppressants (e.g. corticoids, biological therapy).
* Major medical/surgical/psychiatric condition requiring ongoing management. Minor well controlled conditions (i.e. medically controlled arterial hypertension, occupational asthma) may be present.
* Major diagnosis known to chronically affect gut microbiota (e.g. inflammatory bowel disease, liver cirrhosis, colon carcinoma, systemic sclerosis).
* Current diagnosis of a hematological disorder (e.g. severe anemia with hemoglobin \<7 g/dl, leukemia) or any other absolute contraindication for blood donation.
* Participation in other clinical study interfering with study procedures.
* Inability to understand study procedures in order to provide inform consent.
* Previous participation in the same study.
Minimum Eligible Age

18 Years

Maximum Eligible Age

75 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Zurich

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Benjamin Misselwitz, PD Dr.med.

Role: PRINCIPAL_INVESTIGATOR

Division of Gastroenterology, University Hospital Zurich Zurich, Switzerland, 8091

Locations

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Division of Gastroenterology, University Hospital Zurich

Zurich, , Switzerland

Site Status RECRUITING

Countries

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Switzerland

Central Contacts

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Benjamin Misselwitz, PD Dr.med.

Role: CONTACT

+41 44 255 1111

Facility Contacts

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Gerhard Rogler, MD PhD

Role: primary

++41 44 255 9519

Benjamin Misselwitz, MD

Role: backup

++41 44 255 1111

Other Identifiers

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SALMONELLA Study

Identifier Type: -

Identifier Source: org_study_id

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