Dietary Phytoestrogens as Risk Factors for Systemic Lupus Erythematosus

NCT ID: NCT03421184

Last Updated: 2023-07-07

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

63 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-11-26

Study Completion Date

2023-06-26

Brief Summary

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The study aims at determining if dietary phytoestrogens can be risk factors for Systemic Lupus Erythematosus (SLE). Dietary enquiry and phytoestrogens measurements will be performed in blood and urine of patients with SLE in an active phase of the disease, in patient with other autoimmune diseases and in healthy volunteers. Subjects will be premenopausal women and when possible at a define stage of the menstrual cycle. Free blood estradiol will be assayed as a confounding risk factor.

Detailed Description

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SLE is a disease occurring in 90% of the cases in pre-menopausal women. The causing factors are largely unknown even though genetic and environmental factors have already been identified. Estrogens, on one side, have been shown to negatively influence the incidence and severity of the disease while testosterone and progesterone on the other side are thought to be protective. Endocrine disruptors can potentially influence the occurrence and severity of the disease. Among these disruptors, soy isoflavones which are ubiquitous in modern processed food are known to be estrogenic and anti-androgenic. Their depleting effect on luteinizing hormone (LH) secretion can also lead to progesterone plasma level impairments. For these reasons, estrogenic isoflavones, appear as potential environmental risk factors for SLE and its flares. Although some data exist in transgenic rodent, there is actually no clinical data in young women.

The study is an observational, monocentric, preliminary study aiming at determining if estrogenic isoflavones can be risk factors for SLE. No treatment is planned. The intervention will be the collection of extra blood and urine samples on SLE subjects and on autoimmune and healthy counterparts.

Consumers are unintentionally exposed to estrogenic isoflavones through their diet. A dietary habit enquiry and a 48h dietary recall (based on pharmacokinetics of soy-isoflavones) will be proposed to the included subjects. Urine and blood samples collected during a clinical visit performed 7 days after the onset of previous menses will be analyzed for soy isoflavones, metabolites and for enterolactone both free and conjugated. Free estradiol will be assayed as a potential confounding risk factor.

Conditions

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Lupus Erythematosus Rheumatoid Arthritis Autoimmune Thrombocytopenia

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

OTHER

Blinding Strategy

NONE

Study Groups

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Patient with Systemic Lupus Erythematosus

30 women with SLE

Group Type EXPERIMENTAL

blood sample

Intervention Type OTHER

25 ml whole blood for Peripheral blood mononuclear cell (PBMC) and monocytes isolation

urine sample

Intervention Type OTHER

10 ml

food questionnaire

Intervention Type OTHER

dietary habit enquiry and a 48h dietary

hair

Intervention Type OTHER

lock of hair

Patients with other autoimmune diseases

20 patients with rheumatoid arthritis, 20 patients with autoimmune thrombocytopenia

Group Type ACTIVE_COMPARATOR

blood sample

Intervention Type OTHER

25 ml whole blood for Peripheral blood mononuclear cell (PBMC) and monocytes isolation

urine sample

Intervention Type OTHER

10 ml

food questionnaire

Intervention Type OTHER

dietary habit enquiry and a 48h dietary

hair

Intervention Type OTHER

lock of hair

Healthy control

30 healthy control women

Group Type ACTIVE_COMPARATOR

blood sample

Intervention Type OTHER

25 ml whole blood for Peripheral blood mononuclear cell (PBMC) and monocytes isolation

urine sample

Intervention Type OTHER

10 ml

food questionnaire

Intervention Type OTHER

dietary habit enquiry and a 48h dietary

hair

Intervention Type OTHER

lock of hair

Interventions

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blood sample

25 ml whole blood for Peripheral blood mononuclear cell (PBMC) and monocytes isolation

Intervention Type OTHER

urine sample

10 ml

Intervention Type OTHER

food questionnaire

dietary habit enquiry and a 48h dietary

Intervention Type OTHER

hair

lock of hair

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Group : Systemic Lupus Erythematosus :

* Premenopausal women over 18
* with acute LEAD flare
* having given informed consent
* and being covered by social insurance.
* Group : Autoimmune diseases :

* Premenopausal women of matching age with other autoimmune disease,
* having given informed consent
* and being covered by social insurance.
* Healthy controls :

* Premenopausal women over 18,
* having given informed consent,
* and being covered by social insurance.

Exclusion Criteria

* Group Systemic Lupus Erythematosus and group autoimmune diseases

* Human Immunodeficiency virus (HIV), hepatitis C virus (HCV) or hepatitis C virus (HBV) sero-positivity;
* pregnant or lactating women;
* menopausal women;
* patient in remission of quiescent phase of her pathology;
* Healthy controls :

* HIV, HCV or HBV sero-positivity;
* pregnant or lactating women;
* menopausal women.
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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University Hospital, Bordeaux

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Jean-François VIALLARD, Prof

Role: PRINCIPAL_INVESTIGATOR

University Hospital, Bordeaux

Locations

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CHU de Bordeaux - service de médecine interne

Pessac, , France

Site Status

Countries

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France

Other Identifiers

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CHUBX 2017/18

Identifier Type: -

Identifier Source: org_study_id

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