Detection of Anaerobes in Different Clinicalsamples

NCT ID: NCT03266341

Last Updated: 2017-09-01

Basic Information

• Recruitment Status: UNKNOWN
• Total Enrollment: 100 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2017-09-15
• Study Completion Date: 2019-12-31

Brief Summary

Early detection of anaerobic bacteria to avoid its serious comlications

Detailed Description

Infections caused by anaerobic bacteria are common and may be serious and life-threatening.Obligately anaerobic bacteria do not grow on solid media in room air (0.04% carbon dioxide and 21% oxygen); facultatively anaerobic bacteria can grow in the presence or absence of air. Microaerophilic bacteria do not grow at all aerobically or grow poorly, but grow better under 10% carbon dioxide or anaerobically. Anaerobic bacteria can be divided into strict anaerobes that can not grow in the presence of more than 0.5% oxygen and moderate anaerobic bacteria that are able of growing between 2 and 8% oxygen. Anaerobic bacteria usually do not possess catalase, but some can generate superoxide dismutase which protects them from oxygen.

Anaerobes are the predominant components of the bacterial flora of normal human skin and mucous membranes, and are therefore a common cause of bacterial infections of endogenous origin. Infections due to anaerobic bacteria can evolve all body systems and sites . The predominant ones include: abdominal, pelvic, respiratory, and skin and soft tissues infections. Because of their fastidious nature, they are difficult to isolate from infectious sites and are often overlooked. Failure to direct therapy against these organisms often leads to clinical failures. Their isolation requires appropriate methods of collection, transportation, and cultivation of specimens. Treatment of anaerobic bacterial infection is complicated by the slow growth of these organisms, which makes diagnosis in the laboratory possible only after several days, by their often polymicrobial nature and by the growing resistance of anaerobic bacteria to antimicrobial agent.

Almost all anaerobic infections originate from the patient's own microflora. Poor blood supply and tissue necrosis lower the oxidation-reduction potential and favor the growth of anaerobes. Any condition that lowers the blood supply to an affected area can predispose to anaerobic infection. Therefore, foreign body, malignancy, surgery, edema, shock, traum and vascular disease may predispose to anaerobic infection. Previous infection with aerobic or facultative organisms also may make the local tissue conditions more favorable for the growth of anaerobic organisms. The human defense mechanisms also may be impaired by anaerobic conditions .

Conditions

Anaerobic Bacterial Infection

Study Design

• Observational Model Type: CASE_ONLY
• Study Time Perspective: PROSPECTIVE

Interventions

Cultures

Take different samples anddetection of anaerobes on enriched blood agar and other selective anaerobic cultures

Intervention Type: DIAGNOSTIC_TEST

Eligibility Criteria

Inclusion Criteria

• Patient with infection organism not yet detected

Exclusion Criteria

• patients with infection organism is already detected

• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Assiut University

OTHER

Sponsor Role: lead

Responsible Party

Safaa Samir

Detection of anaerobes in different clinical samples

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Sohair Mohamed ahmed, Proffisor

Role: STUDY_DIRECTOR

Proffisor

Asmaa Omar ahmef, Assistant prof

Role: PRINCIPAL_INVESTIGATOR

Assistant pro

Central Contacts

Safaa Ali samir hussien mohamed, Master

Role: CONTACT

Safaa.samir191@gmail.com

01018612254

Other Identifiers

Anaerobes detection in samples

Identifier Type: -

Identifier Source: org_study_id