Safety and Efficiency of γδ T Cell Against Liver Cancer

NCT ID: NCT03183219

Last Updated: 2020-07-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

30 participants

Study Classification

INTERVENTIONAL

Study Start Date

2017-06-15

Study Completion Date

2019-06-15

Brief Summary

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In this study, effects of γδT cells on human Liver Cancer in combination with tumor reducing surgery, for example, cryosurgery going to be investigated.

Detailed Description

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A liver tumor will be removed using tumor reducing surgery such as cryosurgery.PBMC of the patient will be separated from peripheral blood. After making them potential cancer killer γδ T Cell and DC-CIK, they will be infused to the patients as an immunotherapy treatment.

NOTE: Originally, our designed proposal was to use autologous PBMCs from cancer patients to ex vivo expand Vγ9Vδ2-T cells, and then perform adoptive transfer therapy. However, PBMCs of majority of cancer patients could not be effectively expanded, including cell number, cell purity and cell function could not meet the requirements of reinfusion. Meanwhile, those patients could not tolerate 100ml of blood drawing for culture every 2 \~ 3 weeks.

Therefore, we submitted new clinical study application to the ethical committee of the Fuda Hospital affiliated with Jinan University (Guangzhou, PR. China) by changing the autologous protocol with the allogeneic protocol. After the allogeneic protocol was approved, we adapted allogeneic cells instead of autologous in our subsequentially clinical study.

Conditions

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Liver Cancer

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Investigators

Study Groups

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Group A

In this group, the patients will receive under CT Cryosurgery or IRE surgery to control the local tumor.

Group Type EXPERIMENTAL

Cryosurgery or IRE surgery

Intervention Type PROCEDURE

Cryosurgery or IRE surgery will be used in local tumor

Group B

In this group, the patients will receive multiple high-activity γδ T cell immunotherapies. The check indexes are CT scan and blood tests (including tumor markers, lymphocyte subsets and circulating tumor cell).

Group Type EXPERIMENTAL

γδ T cell

Intervention Type BIOLOGICAL

γδ T cells will be used against Liver Cancer

Group C

In this group, the patients will receive multiple high-activity γδ T cell immunotherapies and Cryosurgery or IRE surgery

Group Type EXPERIMENTAL

γδ T cells/ A Cryosurgery or IRE

Intervention Type OTHER

Combination γδ T cell and Cryosurgery or IRE surgery will be used in Liver Cancer

Interventions

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Cryosurgery or IRE surgery

Cryosurgery or IRE surgery will be used in local tumor

Intervention Type PROCEDURE

γδ T cell

γδ T cells will be used against Liver Cancer

Intervention Type BIOLOGICAL

γδ T cells/ A Cryosurgery or IRE

Combination γδ T cell and Cryosurgery or IRE surgery will be used in Liver Cancer

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

1. Age: 18-75
2. Karnofsky performance status \>50
3. Diagnosis with Liver Cancer based on histology or the current accepted radiological measures
4. Classification tumor,nodes,metastasis-classification(TNM) stage: Ⅱ,Ⅲ,Ⅳ
5. Will receive cryosurgery, IRE, gd T cells
6. Life expectancy: Greater than 3 months
7. Ability to understand the study protocol and a willingness to sign a written informed consent document

Exclusion Criteria

1. Patients with other kinds of cancer
2. History of coagulation disorders or anemia
3. Patients with heart disease
Minimum Eligible Age

18 Years

Maximum Eligible Age

75 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Jinan University Guangzhou

OTHER

Sponsor Role collaborator

Fuda Cancer Hospital, Guangzhou

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Jibing Chen, PhD

Role: PRINCIPAL_INVESTIGATOR

Biological treatment center in Fuda cancer hospital Guangzhou, Guangdong, China, 510000

Locations

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Biotherapy center in Fuda cancer hospital

Guangzhou, Guangdong, China

Site Status

Countries

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China

Other Identifiers

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Gd T cell and liver Ca

Identifier Type: -

Identifier Source: org_study_id

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