Effect of Lactobacillus Casei Shirota on the Small Intestinal Microbiota (ROBIN)

NCT ID: NCT03114839

Last Updated: 2017-06-27

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

6 participants

Study Classification

INTERVENTIONAL

Study Start Date

2017-09-04

Study Completion Date

2018-02-28

Brief Summary

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Currently, obtaining samples directly from the small bowel is difficult due to the highly invasive intubation methods used. Research on the effect of dietary probiotics on the gut microbiota is therefore largely dependent on measurement of the microbial composition in fecal samples. At best, the measurement in fecal samples reflects microbial composition of the large intestine. The microbial composition in the small intestine differs substantially from the composition in feces. In addition, many physiological processes that are modulated by dietary probiotics, such as immunoregulation, mainly occur in the small intestine. Therefore, it is vital to study the effects of dietary probiotics on the small intestinal microbiota, as well. Successful sampling of the small intestine has been demonstrated in animals and humans, using the IntelliCap® CR system. The main aim of the current study is to explore and compare the small intestinal microbiota profiles in healthy subjects before and after consumption of a probiotic drink containing Lactobacillus casei strain Shirota (LcS) (Yakult®).

Detailed Description

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The study is a dietary intervention study with a repeated measures design, comparing the upper gastrointestinal microbiota profiles in healthy male subjects before and after consumption of a probiotic drink containing LcS.

This study is explorative in nature. This pilot-study is used to explore trends in microbiota profile shifts in the small intestine. In addition, the investigators would like to explore whether these microbial shifts are similar to those observed in fecal samples. Information derived from this pilot-study can be used to design future intervention studies focusing on either the effect of probiotics on microbial shifts in the small or large intestine.

Conditions

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Healthy

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

The study is a dietary intervention study with a repeated measures design, comparing the upper gastrointestinal microbiota profiles in healthy male subjects before and after consumption of a probiotic drink containing Lactobacillus casei strain Shirota.
Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Dietary Supplement: Lactobacillus casei strain Shirota (LcS)

Group Type EXPERIMENTAL

Lactobacillus casei strain Shirota (LcS)

Intervention Type DIETARY_SUPPLEMENT

A fermented milk containing LcS, 65 ml for oral administration, 1 bottle per day for 7 days. One bottle of the drink contains: 42.9 kcal/182 kJ; less than 0.1 g of fat; 9.6 g of carbohydrate; 0.01 g of sodium; 0.9 g of protein; and at least 6.5 x 10\^9 colony-forming units of LcS.

Interventions

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Lactobacillus casei strain Shirota (LcS)

A fermented milk containing LcS, 65 ml for oral administration, 1 bottle per day for 7 days. One bottle of the drink contains: 42.9 kcal/182 kJ; less than 0.1 g of fat; 9.6 g of carbohydrate; 0.01 g of sodium; 0.9 g of protein; and at least 6.5 x 10\^9 colony-forming units of LcS.

Intervention Type DIETARY_SUPPLEMENT

Other Intervention Names

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Commercial name: Yakult®

Eligibility Criteria

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Inclusion Criteria

1. Male
2. Age 18-30yrs
3. BMI between 18,5-25 kg/m2
4. Healthy as assessed by the NIZO lifestyle and health questionnaire ("Verklaring leefgewoonten en gezondheid").
5. Non-smoking
6. Regular bowel movement (defecation on average once a day, at least 4 times/week).
7. Signed informed consent
8. Access to internet
9. Access to freezer for storage of biological samples
10. Voluntary participation
11. Willing to accept disclosure of the financial benefit of participation in the study to the authorities concerned.
12. Willing to accept use of all encoded data, including publication, and the confidential use and storage of all data for the study for at least 15 years.
13. Willing to accept information-transfer concerning participation in the study, or information regarding health, like laboratory results, findings at anamnesis or physical examination and eventual adverse events to and from his general practitioner.
14. Willing to comply with study procedures.

Exclusion Criteria

1. Alcohol consumption \> 15 units/week and \> 3/day.
2. Allergic to dairy products (milk allergy or lactose intolerance).
3. Carrying a pacemaker or any other (implanted) medical electronic device.
4. Drug abuse.
5. Having diarrhea within two (2) months prior to the study start.
6. Heavy exercise or sports training \> 10 hours/week.
7. History or presence of any clinically important disease or disorder which, in the opinion of the Investigator, may either put the subject at risk because of participation in the study, or influence the results or the subject's ability to participate in the study.
8. Mental status that is incompatible with the proper conduct of the study.
9. Participation in any scientific study with oral, intravenous or inhalatory administration of any substances during two (2) months before study start.
10. Presence of swallowing disorder or problems with gastro-intestinal transit.
11. Reported special diets such as vegetarian, vegan, or macrobiotic.
12. Scheduled for an MRI scan during the study period.
13. Unstable body weight (weight gain or loss \>5kg in the past three (3) months).
14. Use of antibiotics during the six (6) months prior to study start.
15. Use of any prescribed or non-prescribed medication (other than paracetamol) during the three (3) weeks prior to study start.
16. Use of laxatives and probiotic, prebiotic and fiber supplements during the two (2) months prior to study start.
17. Personnel of research institute(s) involved in execution of the study, their partner and their first and second degree relatives
18. Not having a general practitioner
19. Not willing to have an X-ray if the capsule is not recovered from the feces.
Minimum Eligible Age

18 Years

Maximum Eligible Age

30 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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NIZO Food Research

OTHER

Sponsor Role collaborator

Yakult Honsha European Research Center, ESV

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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NIZO Food Research

Ede, Utrecht,, , Netherlands

Site Status RECRUITING

Countries

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Netherlands

Central Contacts

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Sandra ten Bruggencate, PhD

Role: CONTACT

+31318659504

Els Van Hoffen, PhD

Role: CONTACT

+31318659560

Facility Contacts

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Sandra ten Bruggencate, PhD

Role: primary

+31318659504

Other Identifiers

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NL59320.081.16

Identifier Type: -

Identifier Source: org_study_id

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