Effect of Milk on the Vaccination Response

NCT ID: NCT02924246

Last Updated: 2018-09-05

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

108 participants

Study Classification

INTERVENTIONAL

Study Start Date

2016-08-31

Study Completion Date

2017-12-31

Brief Summary

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Rationale: Oral vaccination is known to induce a systemic immune response as well as an immune response in mucosal tissues, and can therefore serve as a model to study systemic and mucosal immunity. In this study, the oral cholera vaccine DukoralĀ® was chosen as model vaccine. The kinetics of the immune response and the interaction with a raw milk matrix have been evaluated in a previous, pilot study (NL49042.081.14). Based on the outcomes of this pilot, in this study oral cholera vaccination will be applied to study the support of immunity by raw milk, compared to heat-treated milk. The study design has been optimised based on previous results: study duration is extended and sample size is based on relevant change and known variation in the primary outcome parameters.

Infections are an important worldwide cause of death, both in elderly and young children. Therefore, support of immunity could help to reduce the incidence of infections. To screen the potential of specific foods or food ingredients to support immunity, oral vaccination can serve as a model. In a previous study, this model was developed using oral cholera vaccination in human adult volunteers. In that study, raw milk was shown to support the immune response to vaccination. In this follow-up study, the effect of raw milk will be compared with pasteurized and ultra-heat treated (UHT) milk.

Objective: To investigate whether pasteurized milk and/or UHT milk are able to enhance the immune response as induced by oral cholera vaccination, in comparison to raw milk and to regular vaccination.

Study design: The study is designed as a single-blind randomized controlled trial of 6 weeks.

Study population: Healthy subjects of 18-50 years of age.

Interventions: 1) Raw milk, obtained from farms that comply to the high quality requirements for production of raw milk, and that has been screened according to the safety criteria for raw milk; 2) commercially available full-fat UHT milk; 3) commercially available full-fat pasteurized milk.

Detailed Description

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Conditions

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Infection

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

SINGLE

Investigators

Study Groups

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Control group

Regular cholera vaccination

Group Type PLACEBO_COMPARATOR

cholera vaccination

Intervention Type BIOLOGICAL

Oral cholera vaccination on day 0 and day 14

Raw milk

Cholera vaccination - raw milk

Group Type ACTIVE_COMPARATOR

cholera vaccination

Intervention Type BIOLOGICAL

Oral cholera vaccination on day 0 and day 14

raw milk

Intervention Type OTHER

raw milk

Pasteurized milk

Cholera vaccination - pasteurized milk

Group Type ACTIVE_COMPARATOR

cholera vaccination

Intervention Type BIOLOGICAL

Oral cholera vaccination on day 0 and day 14

pasteurized milk

Intervention Type OTHER

pasteurized milk

UHT milk

Cholera vaccination - UHT milk

Group Type ACTIVE_COMPARATOR

cholera vaccination

Intervention Type BIOLOGICAL

Oral cholera vaccination on day 0 and day 14

UHT milk

Intervention Type OTHER

UHT milk

Interventions

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cholera vaccination

Oral cholera vaccination on day 0 and day 14

Intervention Type BIOLOGICAL

raw milk

raw milk

Intervention Type OTHER

pasteurized milk

pasteurized milk

Intervention Type OTHER

UHT milk

UHT milk

Intervention Type OTHER

Other Intervention Names

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Dukoral

Eligibility Criteria

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Inclusion Criteria

* Age 18-50 yr
* Signed informed consent
* Availability of internet connection
* Male or female
* Willing to stop blood donation at the blood bank during the study period

Exclusion Criteria

* Currently participating in another clinical trial
* Previous Cholera, Salmonella, or E. coli vaccination
* Tonsillectomy
* Acute gastroenteritis in the past 2 months
* Use of antibiotics in the past 2 months
* Hypersensitivity to the vaccine, to formaldehyde or to any of the excipients (sodium salts)
* Pregnancy or lactating
* Not willing to drink raw milk
* Allergic to milk or lactose-intolerant
* Disease of GI tract, liver, gall bladder, kidneys, thyroid gland
* Immune-compromised
* Use of immunosuppressive drugs
* Drug abuse, and not willing/able to stop this during the study
* Excessive alcohol usage (men: \>4 consumptions/day or \>20 consumptions/week; women: \>3 consumptions/day or \>15 consumptions/week)
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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FrieslandCampina

INDUSTRY

Sponsor Role collaborator

NIZO Food Research

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Els van Hoffen, PhD

Role: PRINCIPAL_INVESTIGATOR

NIZO Food Research

Locations

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NIZO food research

Ede, , Netherlands

Site Status

Countries

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Netherlands

Other Identifiers

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NL56906.081.16

Identifier Type: -

Identifier Source: org_study_id

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