HIV-DNA Dynamics in HIV Monoinfected or HIV/HCV Coinfected Patients

NCT ID: NCT02836782

Last Updated: 2019-07-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

600 participants

Study Classification

OBSERVATIONAL

Study Start Date

2016-04-30

Study Completion Date

2025-04-30

Brief Summary

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New markers of viral activity are now under investigation. Aim of the study is to investigate the efficacy of new antiretroviral drugs by monitoring HIV-DNA dynamics in HIV-positive population. In HIV/HCV coinfected population, the study of HIV reservoir dynamics and the analysis of the prevalence of HCV resistance-associated mutations will help clinicians to improve the management of coinfected patients.

Detailed Description

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New markers of viral activity are now under investigation. Besides HIV-RNA and CD4 cells count, HIV-DNA is an emerging marker of viral reservoir, that seems to be associated with the risk of HIV-related diseases, especially in HCV coinfected patients. New antiretroviral drugs, particularly integrase inhibitors, are expected to decrease residual viremia and, consequently, viral reservoir. Monitoring of HIV-DNA dynamics during antiretroviral treatment could lead to a better management of HIV infected population.

Also, the role of HCV in influencing HIV natural history requires careful monitoring. In particular, with a broader use of direct acting antiretroviral, the analysis of prevalence of HCV resistance associated mutation will be more and more important to manage patients with treatment failure.

Conditions

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HIV

Study Design

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Observational Model Type

ECOLOGIC_OR_COMMUNITY

Study Time Perspective

PROSPECTIVE

Study Groups

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Naive patients

Patients who begin their first antiretroviral regimen. Blood sample withdrawal

Blood sample withdrawal

Intervention Type PROCEDURE

Blood withdrawal for HIV-DNA or HCV resistance test

Experienced patients

Patients with a history of antiretroviral treatment, switching to a new regimen.

Blood sample withdrawal

Blood sample withdrawal

Intervention Type PROCEDURE

Blood withdrawal for HIV-DNA or HCV resistance test

Interventions

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Blood sample withdrawal

Blood withdrawal for HIV-DNA or HCV resistance test

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Persons ≥ 18 years-old;
* Signing an informed consent to study participation and data treatment;
* HIV-positive patients, beginning a new antiretroviral treatment patients or with clinically relevant events;
* HCV-positive patients beginning an antiviral treatment with DAA.

Exclusion Criteria

* Patient's deny
* Clinical contraindications to blood sample withdrawal
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Catholic University of the Sacred Heart

OTHER

Sponsor Role lead

Responsible Party

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Simona Di Giambenedetto

Researcher

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Simona Di Giambenedetto, Researcher

Role: PRINCIPAL_INVESTIGATOR

Catholic University of Sacred Heart

Locations

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Catholic University of Sacred Heart

Rome, , Italy

Site Status RECRUITING

Countries

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Italy

Central Contacts

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Simona Di Giambenedetto, Researcher

Role: CONTACT

0630155366

Facility Contacts

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Simona Di Giambenedetto, Researcher

Role: primary

00390630155366

References

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Kostrikis LG, Touloumi G, Karanicolas R, Pantazis N, Anastassopoulou C, Karafoulidou A, Goedert JJ, Hatzakis A; Multicenter Hemophilia Cohort Study Group. Quantitation of human immunodeficiency virus type 1 DNA forms with the second template switch in peripheral blood cells predicts disease progression independently of plasma RNA load. J Virol. 2002 Oct;76(20):10099-108. doi: 10.1128/jvi.76.20.10099-10108.2002.

Reference Type BACKGROUND
PMID: 12239284 (View on PubMed)

Shiramizu B, Paul R, Williams A, Shikuma C, Watters M, Grove J, Valcour V. HIV proviral DNA associated with decreased neuropsychological function. J Neuropsychiatry Clin Neurosci. 2007 Spring;19(2):157-63. doi: 10.1176/jnp.2007.19.2.157.

Reference Type BACKGROUND
PMID: 17431062 (View on PubMed)

Asselah T, Boyer N, Saadoun D, Martinot-Peignoux M, Marcellin P. Direct-acting antivirals for the treatment of hepatitis C virus infection: optimizing current IFN-free treatment and future perspectives. Liver Int. 2016 Jan;36 Suppl 1:47-57. doi: 10.1111/liv.13027.

Reference Type BACKGROUND
PMID: 26725897 (View on PubMed)

Bagaglio S, Uberti-Foppa C, Messina E, Merli M, Hasson H, Andolina A, Galli A, Lazzarin A, Morsica G. Distribution of natural resistance to NS3 protease inhibitors in hepatitis C genotype 1a separated into clades 1 and 2 and in genotype 1b of HIV-infected patients. Clin Microbiol Infect. 2016 Apr;22(4):386.e1-386.e3. doi: 10.1016/j.cmi.2015.12.007. Epub 2015 Dec 17.

Reference Type BACKGROUND
PMID: 26706617 (View on PubMed)

Di Maio VC, Cento V, Di Paolo D, Aragri M, De Leonardis F, Tontodonati M, Micheli V, Bellocchi MC, Antonucci FP, Bertoli A, Lenci I, Milana M, Gianserra L, Melis M, Di Biagio A, Sarrecchia C, Sarmati L, Landonio S, Francioso S, Lambiase L, Nicolini LA, Marenco S, Nosotti L, Giannelli V, Siciliano M, Romagnoli D, Pellicelli A, Vecchiet J, Magni CF, Babudieri S, Mura MS, Taliani G, Mastroianni C, Vespasiani-Gentilucci U, Romano M, Morisco F, Gasbarrini A, Vullo V, Bruno S, Baiguera C, Pasquazzi C, Tisone G, Picciotto A, Andreoni M, Parruti G, Rizzardini G, Angelico M, Perno CF, Ceccherini-Silberstein F; HCV Italian Resistance Network Study Group. HCV NS3 sequencing as a reliable and clinically useful tool for the assessment of genotype and resistance mutations for clinical samples with different HCV-RNA levels. J Antimicrob Chemother. 2016 Mar;71(3):739-50. doi: 10.1093/jac/dkv403. Epub 2015 Dec 17.

Reference Type BACKGROUND
PMID: 26679249 (View on PubMed)

AASLD/IDSA/IAS-USA RETREATMENT OF PERSONS IN WHOM PRIOR THERAPY HAS FAILED. Recommendations for testing, managing, and treating hepatitis C. date accessed 5.01. 2015.

Reference Type BACKGROUND

Fourati S, Pawlotsky JM. Virologic Tools for HCV Drug Resistance Testing. Viruses. 2015 Dec 4;7(12):6346-59. doi: 10.3390/v7122941.

Reference Type BACKGROUND
PMID: 26690198 (View on PubMed)

Perales C, Quer J, Gregori J, Esteban JI, Domingo E. Resistance of Hepatitis C Virus to Inhibitors: Complexity and Clinical Implications. Viruses. 2015 Nov 6;7(11):5746-66. doi: 10.3390/v7112902.

Reference Type BACKGROUND
PMID: 26561827 (View on PubMed)

Lombardi F, Belmonti S, Borghetti A, Fabbiani M, Marchetti S, Tamburrini E, Cauda R, di Giambenedetto S. Evolution of cellular HIV DNA levels in virologically suppressed patients switching to dolutegravir/lamivudine versus maintaining a triple regimen: a prospective, longitudinal, matched, controlled study. J Antimicrob Chemother. 2020 Jun 1;75(6):1599-1603. doi: 10.1093/jac/dkaa058.

Reference Type DERIVED
PMID: 32101614 (View on PubMed)

Other Identifiers

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Prot.7768/16 ID 1086

Identifier Type: -

Identifier Source: org_study_id

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