Study to Obtain Blood and Voided Urine Samples to Improve the Diagnosis of Melioidosis

NCT ID: NCT02668406

Last Updated: 2018-10-23

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

4203 participants

Study Classification

OBSERVATIONAL

Study Start Date

2016-02-29

Study Completion Date

2018-09-30

Brief Summary

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Clinical samples \[blood and voided urine (only for phase A)\] from patient recruited at Sihanouk Hospital Centre of HOPE (SHCH) and HOPE Community Medical Center (CMC) will be processed (decontamination) and shipped to SRI International with the purpose of design and validation (proof of concept) and (case/control series) of in-vitro diagnostics for melioidosis.

Detailed Description

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Study will be proposed to all patients at Sihanouk Hospital Centre of HOPE (SHCH) and HOPE Community Medical Center (CMC) presenting with systemic inflammatory response syndrome (SIRS).

Blood samples will be collected upon admission for patients with blood culture request as part of routine patient care and agreement on informed consent. A second sample will be collected in patients with blood culture proven melioidosis or suspicion of melioidosis (only for phase B), at day 5 after initiation of appropriate antibiotic treatment. Depending on the final diagnosis, a subset of patients will be assigned to 3 groups:

1. Patients for whom blood cultures grew Burkholderia pseudomallei
2. Patients for whom blood cultures did not grow a pathogen, but have other body site grown with Burkholderia pseudomallei, or are suspected of melioidosis
3. Patients for whom blood cultures grew with another pathogen

In phase A only, at least 20 ml of voided urine will be collected when a patient is hospitalized and identified as belonging to study group 1, 2 or 3. A second collection of voided urine will be collected for group 1 and 2 patients during routine further hospital stay or a routine follow-up visit.

The clinical samples from these patients will be processed and shipped to SRI International with the purpose of design (=phase A) and validation (=phase B) of in-vitro diagnostics for melioidosis.

A coded database will be completed with basic demographic, clinical and microbiological data and final diagnosis. This coded information will be provided to SRI International.

Conditions

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Melioidosis Systemic Inflammatory Response Syndrome Suspected or Confirmed Bloodstream Infections

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Burkholderia pseudomallei

Patients for whom blood cultures grew Burkholderia pseudomallei

Venous blood sampling (2 timepoints) (Phase A and B)

Intervention Type OTHER

8.5ml at Day 1 + 8.5ml at Day 5

Voided urine sampling (first sample) (Phase A)

Intervention Type OTHER

At least 20ml upon confirmation of being part of one of the 3 study groups

Voided urine sampling (second sample) (Phase A)

Intervention Type OTHER

At least 20ml during routine hospital stay or routine follow-up visit

No pathogen

Patients for whom blood cultures did not grow a pathogen, but have other body site grown with Burkholderia pseudomallei, or are suspected of melioidosis

Venous blood sampling (2 timepoints) (Phase A and B)

Intervention Type OTHER

8.5ml at Day 1 + 8.5ml at Day 5

Voided urine sampling (first sample) (Phase A)

Intervention Type OTHER

At least 20ml upon confirmation of being part of one of the 3 study groups

Voided urine sampling (second sample) (Phase A)

Intervention Type OTHER

At least 20ml during routine hospital stay or routine follow-up visit

Another pathogen

Patients for whom blood cultures grew another pathogen

Venous blood sampling (1 timepoint) (Phase A and B)

Intervention Type OTHER

8.5ml at Day 1

Voided urine sampling (first sample) (Phase A)

Intervention Type OTHER

At least 20ml upon confirmation of being part of one of the 3 study groups

Interventions

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Venous blood sampling (2 timepoints) (Phase A and B)

8.5ml at Day 1 + 8.5ml at Day 5

Intervention Type OTHER

Venous blood sampling (1 timepoint) (Phase A and B)

8.5ml at Day 1

Intervention Type OTHER

Voided urine sampling (first sample) (Phase A)

At least 20ml upon confirmation of being part of one of the 3 study groups

Intervention Type OTHER

Voided urine sampling (second sample) (Phase A)

At least 20ml during routine hospital stay or routine follow-up visit

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Adult (\>18 years old)
* Blood culture request by treating physician as part of standard care
* Willing to give informed consent

Exclusion Criteria

* Refusal to give informed consent
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Sihanouk Hospital Center of HOPE

OTHER

Sponsor Role collaborator

SRI International

INDUSTRY

Sponsor Role collaborator

Institute of Tropical Medicine, Belgium

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Jan Jacobs, MD

Role: STUDY_DIRECTOR

Institute of Tropical Medicine, Antwerp, Belgium

Thong Phe, MD

Role: PRINCIPAL_INVESTIGATOR

Sihanouk Hospital Center of HOPE (SHCH), Cambodia

Locations

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HOPE Community Medical Center

Phnom Penh, , Cambodia

Site Status

Sihanouk Hospital Center of HOPE (SHCH), Cambodia

Phnom Penh, , Cambodia

Site Status

Countries

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Cambodia

Other Identifiers

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Melioidosis

Identifier Type: -

Identifier Source: org_study_id

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