Study of Dendrogenin A / Oxysterols Balance Between Healthy Volunteers and Acute Myeloid Leukemia Patients

NCT ID: NCT02602587

Last Updated: 2019-05-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

37 participants

Study Classification

INTERVENTIONAL

Study Start Date

2014-11-25

Study Completion Date

2018-11-20

Brief Summary

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Dendrogenin A is present in mammals normal tissues and fluids, notably blood. It is down-represented or absent in cancer cell lines and tumors. In the opposite, OCDO is accumulated in cancerous conditions and virtually absent of normal tissues. This study will try to determine modulations of these oxysterols and protein involved in their metabolism between Acute myeloid leukemia (AML) patient samples and normal blood or marrow, for evaluation of these markers as companion biomarkers for Dendrogenin A treatment.

Detailed Description

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Background : Acute myeloid leukemia is an unmet medical need since 5-year overall survival is 40-50% in younger adults and 10-15% in elderly. Dendrogenin A is a natural novel anticancer agent showing potent antileukemic activity which is under-represented in tumors. Dendrogenin A inhibits Cholesterol epoxide hydrolase and so modulates levels of substrates and products of this enzymatic complex.

Purpose : This is a biomedical study of interventional type which includes 30 patients and 30 blood samples as well as 5 marrow samples from healthy donors. It will last 24 month, 8 months recruiting and 16 months follow-up. Patients with acute myeloid leukemia for which an indication of chemotherapy treatment is retained will be enrolled in this study. This study will compare oxysterols (Dendrogenin A, 6-oxo-cholestane-3beta,5alpha-diol - OCDO) and related proteins as biomarkers of the disease between the two populations.

Interventions : The patients included in this study will be processed according to the standard treatment in force for their disease. This study does not in any way interfere with this treatment regimen, and is only based on additional samples of blood and bone marrow in acts planned in the prognostic or follow-up protocols. Treatment shall start within the 15 days following inclusion and first sampling.

Conditions

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Acute Myeloid Leukemia

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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blood samples and bone marrow samples

The patients included in this study will be processed according to the standard treatment in force for their disease. This study does not in any way interfere with this treatment regimen, and is only based on additional samples of blood and bone marrow in acts planned in the prognostic or follow-up protocols. Treatment shall start within the 15 days following inclusion and first sampling.

Group Type EXPERIMENTAL

Blood sample

Intervention Type BIOLOGICAL

additional samples of blood in acts planned in the prognostic or follow-up protocols.

Bone marrow sample

Intervention Type BIOLOGICAL

additional bone marrow sample in acts planned in the prognostic or follow-up protocols.

Interventions

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Blood sample

additional samples of blood in acts planned in the prognostic or follow-up protocols.

Intervention Type BIOLOGICAL

Bone marrow sample

additional bone marrow sample in acts planned in the prognostic or follow-up protocols.

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* Men or women of 18 years old or older
* Informed consent obtained and signed before any specific procedure in the study
* Patient member in a national insurance scheme

\* For patients with AML
* Men or women with a non promyelocytic acute myeloid leukemia as defined by WHO criteria and with at least 20% leukemic cells in blood at diagnosis
* for which treatment combining cytarabine and daunorubicin or idarubicin is retained
* WHO performance status ≤ 2

* for healthy volunteers Criteria of eligibility for blood or bone marrow donation according to French Blood Institution

Exclusion Criteria

* Pregnant women or nursing mothers cannot participate in the study.
* Patients under legal guardianship.
* Any other medical or psychiatric condition or laboratory abnormality severe, acute or chronic making the inclusion of the patient in the study inappropriate in the opinion of the investigator.
* Patient unable to follow procedures, visits, examinations described in the study.

\* For patients with AML :
* Patients having received any anti-AML treatment with the exception of oral hydroxyurea.
* Patients for which a treatment different from the combination cytarabine+anthracycline has been selected

\* For healthy volunteers
* Criteria of ineligibility for blood donation according to EFS (French Blood Institution)
* For bone marrow : heart disease, high blood pressure, respiratory diseases, diseases of the nervous system history of cancers, diseases metabolic (diabetes, liver failure), history of recurring phlebitis or pulmonary embolism, disabling lumbar problems, neuromuscular diseases, angioedema, significant overweight,.
* Any long term treatment, except contraception
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University Hospital, Toulouse

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Christian RECHER, Doctor

Role: PRINCIPAL_INVESTIGATOR

University Hospital, Toulouse

Locations

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Chu Toulouse

Toulouse, , France

Site Status

Countries

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France

Other Identifiers

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14 7166 03

Identifier Type: -

Identifier Source: org_study_id

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