Adenosylmethionine Metabolism in Human Inflammation

NCT ID: NCT02520206

Last Updated: 2015-08-11

Basic Information

• Recruitment Status: UNKNOWN
• Total Enrollment: 250 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2011-01-31
• Study Completion Date: 2015-08-31

Brief Summary

The investigators propose to conduct a translational study on the regulation of S-adenosylmethionine synthesis and cellular methylation reactions during chronic inflammation. Development of in vitro cell models may reveal the regulatory mechanisms by which specific inflammatory mediators cause metabolic changes and alter DNA methylation status. Metabolic and pharmacological studies in the in vivo models will enable us to better understand the regulation of inter-organ homeostasis of S-adenosyl methionine and help identify tissue specific biomarkers for methylation and epigenetic modifications in different stage of chronic inflammation. The clinical study in human subjects will help distinguish the impacts of autoimmune rheumatic disease, degenerated joint disease, or specific medication use on significant clinical and biochemical markers in folate and vitamin B6 metabolic pathways.The Investigators hope the present study can identify specific clinical markers for potential epigenetic changes in patients suffering from chronic inflammation, which will contribute to better clinical management of these diseases in humans.

Detailed Description

The significance of epigenetic alterations in autoimmune rheumatic diseases and degenerated joint diseases has drawn great attention among clinicians and researchers. Aberrant methylation status has been demonstrated in human chronic inflammation yet more efforts have focused on global and sequence-specific hypomethylation and overexpression of specific genes. Few studies investigated the regulation of S-adenosylmethionine homeostasis and regulation during inflammation. At present the relevance and regulation of the complex epigenetic profiles and their modifications among different tissues and organs during inflammation remain largely unknown.

Conditions

Arthritis; Chronic Inflammation

Study Groups

Arthritis

subjects with arthritis

No interventions assigned to this group

Health control subjects

Health control

No interventions assigned to this group

Eligibility Criteria

Inclusion Criteria

• > 18 years

Exclusion Criteria

• pregnancy,
• anemia (hemoglobin 10 mg/dL or lower),
• thrombocytopenia (platelet count below 50,000 cells/μL),
• abnormal serum hepatic transaminase (aspartate aminotransferase or alanine aminotransferase above 50 IU/L),
• diabetes or cancer

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 80 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

National Science and Technology Council, Taiwan

OTHER_GOV

Sponsor Role: collaborator

Ministry of Science and Technology, Taiwan

OTHER_GOV

Sponsor Role: collaborator

National Chung Hsing University

OTHER

Sponsor Role: lead

Responsible Party

En-Pei Isabel Chiang

Professor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

En-Pei I Chiang, PhD

Role: PRINCIPAL_INVESTIGATOR

Department of Food Science and Biotechnology, National Chung Hsing University

Other Identifiers

SF11093

Identifier Type: -

Identifier Source: org_study_id