L-leucine in Diamond Blackfan Anemia Patients

NCT ID: NCT02386267

Last Updated: 2015-03-11

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

PHASE2

Total Enrollment

30 participants

Study Classification

INTERVENTIONAL

Study Start Date

2014-09-30

Study Completion Date

2016-03-31

Brief Summary

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Diamond-Blackfan anemia (DBA) is a rare congenital syndrome associated with physical anomalies, short stature, red cell aplasia, and an increased risk of malignancy.

Mutations affecting genes encoding ribosomal proteins cause DBA. Genetic studies have identified heterozygous mutations in at least one of eight ribosomal protein genes in up to 50% of cases.

25% of patients carry a mutation in the ribosomal protein (RP)S19 gene, whereas mutations in RPS24, RPS17, RPL35A, RPL11, and RPL5 are rare.

p53 activation has been identified as a key component in the pathophysiology of DBA after cellular and molecular studies. Other potential mechanisms that warrant further investigation include impaired translation as the result of ribosomal insufficiency, which may be ameliorated by Leucine supplementation.

Despite significant improvements in understanding of the pathophysiology of Diamond Blackfan anemia (DBA), there have been few advances in therapy. The cornerstones of treatment remain corticosteroids,chronic red blood cell transfusions, and hematopoietic stem cell transplantation, each of which is fraught with complications. Other treatments have been shown to be effective in only a few patients or in individual case reports : IL-3, cyclosporine (alone or in combination with steroids), metaclopramide. Gene therapy is still a part of research programs.

There are some indications that the Amino Acid (AA) L-leucine, a translation enhancer, may have some efficacy in DBA and 5q-syndrome, which has the same altered ribosome functions as the DBA. L-leucine is an essential AA that is unique among the branched-chain AA acting as a nutrient regulator of protein synthesis in skeletal muscle and adipose tissue.

Several preclinical studies with DBA lymphocytes exposed to various L-leucine doses, have demonstrated that protein synthesis can be increased by using high doses L-leucine.

Recent clinical data on L-leucine therapeutic use have demonstrated increase the hemoglobin level and transfusion independence in patients with DBA and 5q-syndrom.

These data support the rationale for clinical trial on L-leucine use as a therapeutic agent for DBA patients.

Detailed Description

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Experimental: one arm L-leucine , dose- 700mg/m2 , per os, 3 time a day, 6 months

Conditions

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Diamond Blackfan Anemia

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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L-leucine pills

L-leucine , dose- 700mg/m2 , per os, three time a day, course duration 6 months

Group Type EXPERIMENTAL

L-leucine

Intervention Type DRUG

L-leucine pills per os for 6 months

Interventions

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L-leucine

L-leucine pills per os for 6 months

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* signed Informed Consent Form
* diagnosed Diamond Blackfan Anemia
* transfusion dependenсe
* adequate renal function
* adequate liver function
* negative B-HCG and adequate contraception

Exclusion Criteria

* known hypersensitivity to branched chain amino acids
* diagnosed AA metabolism disorder
* prior HSCT
* pregnancy or planning to become pregnant
Minimum Eligible Age

1 Year

Maximum Eligible Age

20 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Federal Scientific Clinical Centre of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogache

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Natalia - SMETANINA, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

FSCCPHOI, Outpatient Department

Locations

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Federal Scientific Clinical Centre of Pediatric Hematology Oncology Immunology n.a. Dmitry Rogachev

Moscow, , Russia

Site Status RECRUITING

Countries

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Russia

Central Contacts

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Natalia - SMETANINA, MD, PhD

Role: CONTACT

+7 985 647 13 05 ext. 13 05

Galina - OVSYANNIKOVA, PhD

Role: CONTACT

+7 916 238 13 57 ext. 1710

Facility Contacts

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Natalia - SMETANINA, MD, PhD

Role: primary

+7 985 647 13 05 ext. 13 05

Other Identifiers

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Ru0001

Identifier Type: -

Identifier Source: org_study_id

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