Cardiovascular Disease Protection Tissue

NCT ID: NCT02348515

Last Updated: 2018-11-27

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

Get a concise snapshot of the trial, including recruitment status, study phase, enrollment targets, and key timeline milestones.

Recruitment Status

COMPLETED

Total Enrollment

8 participants

Study Classification

OBSERVATIONAL

Study Start Date

2013-06-30

Study Completion Date

2018-09-28

Brief Summary

Review the sponsor-provided synopsis that highlights what the study is about and why it is being conducted.

Recent evidence of a potential role for cardiac progenitor cells (CPCs) in cardiac repair and the discovery of a vasoprotective axis of the renin-angiotensin system (RAS) offer such breakthroughs. Investigators have observed that an imbalance in the vasoprotective axis {angiotensin converting enzyme 2 (ACE2)/angiotensin-(1-7) \[Ang-(1-7)\]/Mas receptor} and the vasodeleterious axis \[angiotensin converting enzyme (ACE)/angiotensin II (AngII)/AngII type 1 receptor (AT1R)\] of the RAS within the CPCs affects their functionality and regenerative potential. Investigators believe that restoring the balance between these two axes of the RAS is essential to improve CPC function and enhance their reparative capabilities. These observations have led to the hypothesis that genetic modification of CPCs by overexpression of ACE2/Ang-(1-7) will enhance their reparative function and improve their potential to attenuate myocardial ischemia-induced cardiac damage.

Detailed Description

Dive into the extended narrative that explains the scientific background, objectives, and procedures in greater depth.

As a participant undergoes a clinically indicated heart transplant, or a left ventricular assist device (LVAD) implantation, or a right heart biopsy, or atrial fibrillation surgery, or right atria cannulation, the following tissue samples will be collected:

In the subjects undergoing orthotopic heart transplant (n=20), failed myocardial tissue samples will be collected from the diseased heart.

For subjects undergoing left ventricular assist device implantation (n=60), small samples will be collected from the apex core (that would be routinely discarded at the time of the implantation procedure).

For heart transplant subjects undergoing clinically indicated right heart biopsy (n=20), the collection of multiple samples including, excess myocardial biopsy samples that will not be utilized by pathology.

For subjects undergoing any heart surgery (n=80), the collection of left atrial appendages that are routinely removed to prevent thrombosis during atrial fibrillation surgery and a piece of the right atria will be cut in order to implant the cannula.

In addition, a collection of 20ml (about one tablespoon) of blood will be taken from all subjects to analyze progenitor/inflammatory cells and inflammation cytokines and pertinent medical history.

Conditions

See the medical conditions and disease areas that this research is targeting or investigating.

Myocardial Ischemia

Study Design

Understand how the trial is structured, including allocation methods, masking strategies, primary purpose, and other design elements.

Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

Review each arm or cohort in the study, along with the interventions and objectives associated with them.

Heart failure or coronary disease

This group will have small samples collected from the apex core (that would be routinely discarded at the time of the implantation procedure) by undergoing a left ventricular assist device implantation. In addition, a blood sample will be collected.

Heart failure or coronary disease

Intervention Type PROCEDURE

Small samples collected from the apex core in the heart. In addition, blood samples will be taken.

Blood Draw

Intervention Type PROCEDURE

All subjects will have 20 ml of blood drawn for further analysis.

Heart transplant patients

This group will have multiple samples collected including, excess myocardial biopsy samples that will not be utilized by pathology. In addition, a blood sample will be collected.

Heart transplant patients

Intervention Type PROCEDURE

Heart samples collected including, excess myocardial biopsy samples. In addition, blood samples will be taken.

Blood Draw

Intervention Type PROCEDURE

All subjects will have 20 ml of blood drawn for further analysis.

Orthotopic Heart Transplant Patients

This group will have myocardial tissue samples collected from the diseased heart. In addition, a blood sample will be taken.

Orthotopic Heart Transplant Patients

Intervention Type PROCEDURE

Myocardial tissue samples collected from the diseased heart. In addition, blood samples will be taken.

Blood Draw

Intervention Type PROCEDURE

All subjects will have 20 ml of blood drawn for further analysis.

Heart Surgery Patients

This groups will have samples collected from the left atrial appendages that are routinely removed to prevent thrombosis during atrial fibrillation surgery and a piece of the right atria will be cut in order to implant the cannula. In addition, a blood sample will be taken.

Heart Surgery Patients

Intervention Type PROCEDURE

Heart samples will be collected from the left atrial appendages. In addition, blood samples will be taken.

Blood Draw

Intervention Type PROCEDURE

All subjects will have 20 ml of blood drawn for further analysis.

Interventions

Learn about the drugs, procedures, or behavioral strategies being tested and how they are applied within this trial.

Heart failure or coronary disease

Small samples collected from the apex core in the heart. In addition, blood samples will be taken.

Intervention Type PROCEDURE

Heart transplant patients

Heart samples collected including, excess myocardial biopsy samples. In addition, blood samples will be taken.

Intervention Type PROCEDURE

Orthotopic Heart Transplant Patients

Myocardial tissue samples collected from the diseased heart. In addition, blood samples will be taken.

Intervention Type PROCEDURE

Heart Surgery Patients

Heart samples will be collected from the left atrial appendages. In addition, blood samples will be taken.

Intervention Type PROCEDURE

Blood Draw

All subjects will have 20 ml of blood drawn for further analysis.

Intervention Type PROCEDURE

Eligibility Criteria

Check the participation requirements, including inclusion and exclusion rules, age limits, and whether healthy volunteers are accepted.

Inclusion Criteria

* heart transplant surgery
* left ventricular assist device implantation
* heart surgery required for atrial fibrillation and right atria cannulation
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

Meet the organizations funding or collaborating on the study and learn about their roles.

National Institutes of Health (NIH)

NIH

Sponsor Role collaborator

National Heart, Lung, and Blood Institute (NHLBI)

NIH

Sponsor Role collaborator

University of Florida

OTHER

Sponsor Role lead

Responsible Party

Identify the individual or organization who holds primary responsibility for the study information submitted to regulators.

Responsibility Role SPONSOR

Principal Investigators

Learn about the lead researchers overseeing the trial and their institutional affiliations.

Carl J Pepine, MD

Role: PRINCIPAL_INVESTIGATOR

University of Florida

Locations

Explore where the study is taking place and check the recruitment status at each participating site.

Yanfei Qi

Gainesville, Florida, United States

Site Status

Countries

Review the countries where the study has at least one active or historical site.

United States

Other Identifiers

Review additional registry numbers or institutional identifiers associated with this trial.

R01HL056921

Identifier Type: NIH

Identifier Source: secondary_id

View Link

IRB201300122-N

Identifier Type: -

Identifier Source: org_study_id

More Related Trials

Additional clinical trials that may be relevant based on similarity analysis.

J-Valve TF Early Feasibility Study
NCT06034028 ACTIVE_NOT_RECRUITING NA
Prince of Wales Hospital Structural Heart Registry
NCT07020286 ENROLLING_BY_INVITATION