Abnormal Lipids - Causes and Effects

NCT ID: NCT02195050

Last Updated: 2023-05-31

Basic Information

• Recruitment Status: RECRUITING
• Total Enrollment: 1396 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2014-01-23
• Study Completion Date: 2030-12-31

Brief Summary

1. At target LDL-C levels, apoB100 concentrations will be higher than recommended levels in the following populations:

1. Tertiary centre lipid clinic patients with raised TG treated with statins.

2. Patients with type 2 diabetes treated with statins.

3. Patients with Chronic Kidney disease (CKD) stages 4 and 5 treated with statins.

2. Despite achieving LDL-C and non-HDL-C targets, a significant number of statin-treated patients have residual cardiovascular risk related to raised hsCRP. The relationship between hsCRP and Lp-PLA2 (markers of inflammation) and LDL particle number measured by apoB100 is stronger than that of measured and calculated LDL and non-HDL. In statin treated patients there will be higher levels of hs-CRP and Lp-PLA2 in patients achieving LDL targets but not apo B targets.

3. We hypothesise that non-diabetic patients with severe hypertriglyceridaemia (fasting serum triglyceride >5.5 mmol/l) have evidence of greater nerve damage compared with matched controls.

4. LAL deficiency is underdiagnosed in patients with severe hypertriglyceridaemia, low HDL-C, hyperlipidaemias, non alcoholic fatty liver disease and idiopathic high liver enzymes.

Detailed Description

Fats are present in the body in the form of lipid particles containing cholesterol and triglycerides. Lipid particles can deposit in blood vessels, forming atheromas, blocking blood vessels and leading to heart attacks and strokes. These harmful particles are termed 'atherogenic' particles. Low density lipoprotein (LDL) is the major atherogenic particle. Each atherogenic particle has a protein associated with it called apolipoprotein B (apoB). Cholesterol, triglycerides, and apoB levels can be measured in the laboratory. In this study we focus on patients with raised levels of triglycerides (hypertriglyceridaemia).

We will recruit patients with hypertriglyceridaemia to look at (1) therapeutic targets, (2) genetic causes, and (3) associated neuropathy in these patients.

1. Therapeutic target arm:

LDL-cholesterol (LDL-C) is the primary target for lipid-lowering drugs. A drug (commonly a statin) is effective when it lowers LDL-C. Patients with diabetes, chronic kidney disease and hypertriglyceridaemia on statins may have normal levels of LDL-C; but because the size of LDL particles in these patients is smaller, they may in fact have raised levels of apoB and therefore remain at cardiovascular risk. Thus, measurement of apoB may be a better target for treatment because it measures 'atherogenic' particle numbers. Since atheromatous disease is an inflammatory disease, we will also investigate if residual risk may be correlated with inflammatory markers, such as hsCRP and Lp-PLA2.

2. Hypertriglyceridaemia and nerve function arm:

We aim to demonstrate that severe hypertriglyceridaemia is associated with more significant nerve damage.

3. Genetic screening arm:

Several genetic mutations have been identified to cause hypertriglyceridaemia. Deficiency of the enzyme lysosomal acid lipase (LAL) results in Cholesterol Ester Storage Disease (CESD) and hypercholesterolaemia, hypertriglyceridaemia, and abnormal lipid deposition in organs. We wish to identify genetic mutations associated with severe hypertriglyceridemia and the prevalence of LAL deficiency in patients attending our tertiary referral centre.

Conditions

Hypertriglyceridaemia

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: CROSS_SECTIONAL

Study Groups

Therapeutic target arm

Statin treated patients with and without raised triglycerides who do not have diabetes or dysglycemia. Statin treated patients with type 2 diabetes.Statin treated patients with CKD stages 4 and 5 (eGFR ≤30mL/min).

No interventions assigned to this group

Nerve function arm

Patients with severe hypertriglyceridaemia (fasting TG \> 5.5mmol/l.) are recruited for nerve function assessment and corneal confocal microscopy.

No interventions assigned to this group

Genetic screening arm

For LAL deficiency screening, patients will be recruited over a 5 year period with a documented triglyceride level of more than 10 mmol/l at any time, low HDLC, raised ALT, combined hyperlipidaemia, or non-alcoholic fatty liver disease. Patients recruited from Manchester will be offered additional genetic testing for familial hypercholesterolaemia.

No interventions assigned to this group

Eligibility Criteria

Inclusion Criteria

• Therapeutic target arm

• Statin treated patients with and without hypertriglyceridemia.
• Statin treated patients with type 2 diabetes.
• Statin treated patients with CKD stages 4 and 5.
• Nerve function arm

•Patients known to have severe hypertriglyceridaemia (defined as triglyceride >5.5 mmol/l) but not known to have diabetes and matched controls.

• Genetic screening arm

• Patients with a documented triglyceride level of more than 10 mmol/l at any time.
• Criteria for screening for FH and LAL deficiency include non-obese patients (BMI <30) with low HDL-C (<1.0 mmol/l male and <1.3 mmol female), high triglycerides >1.7 mmol/l, high total cholesterol >6.2 or LDL cholesterol >4.7 mmol/l; patients with raised liver alanine aminotransferase (ALT) (1.5 x above ULN) but no metabolic or viral disease or alcohol excess and patients diagnosed with NAFLD with or without hyperlipidaemia.

Exclusion Criteria

• Pregnant and/or breast-feeding women.
• Significant liver impairment.
• Patients known to have active malignant disease.
• Patients treated with medications that could affect lipoprotein metabolism significantly (like atypical antipsychotics, chemotherapy).
• Untreated hypothyroid and hyperthyroidism (if treated and TFT normal could be recruited).

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Manchester University NHS Foundation Trust

OTHER_GOV

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Handrean Soran, MD FRCP

Role: PRINCIPAL_INVESTIGATOR

Manchester University NHS Foundation Trust

Locations

Cardiovascular Trials Unit

Manchester, , United Kingdom

Site Status: RECRUITING

Countries

United Kingdom

Central Contacts

See Kwok, MD FRCGP

Role: CONTACT

sk7@doctors.org.uk

01612768863

Facility Contacts

See Kwok, MD FRCGP

Role: primary

sk7@doctors.org.uk

01612768863

Other Identifiers

R03301

Identifier Type: OTHER

Identifier Source: secondary_id

APOB2012

Identifier Type: -

Identifier Source: org_study_id