Search of Biomarkers of Mineralocorticoid Receiver Activity : the Value of Neutrophil Gelatinase Associated Lipocalin (NGAL)in Serum or Urine in Human

NCT ID: NCT02129621

Last Updated: 2014-05-02

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 42 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2010-05-31
• Study Completion Date: 2012-11-30

Brief Summary

Aldosterone and its target the mineralocorticoid receptor are implied in development of cardiovascular disease such as arterial high blood pressure or cardiac failure. Serum aldosterone level is insufficient to know if the mineralocorticoid receptor is activated or not. The aim of this study is to identify biomarkers of mineralocorticoid receptor activity. The first "candidate biomarker" is Neutrophil gelatinase-associated lipocalin (NGAL) or "lipocalin" which being measured in blood or in the urine. According to scientific knowledge's evolution, others biomarkers could be tested later thanks to the blood or urinary samples taken during study.

Detailed Description

This study is broken up into 5 groups:

The first is a pre-analytical study to determinate optimum conditions for lipocalin samples.

The second focuses on intra-individual variations of lipocalin levels in blood and urine.

The third determinate the normal values of lipocalin in blood and urine. Fourthly, we study the role of renal function on serum and urines lipocalin levels.

Fifthly, we study the role of anti-aldosterone treatment on patients who suffer of primary hyperaldosteronism.

Conditions

Cardiovascular Diseases

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: DIAGNOSTIC
• Blinding Strategy: NONE

Interventions

Urinary and blood lipococalin levels determination

Blood test on an empty stomach. Urinary taking on arrival in the service

Intervention Type: PROCEDURE

Spironolactone

For the group 5, at the time of the V0 visit, a treatment by aldactone will be set up (posology: 1 to 2 mg/kg).

Intervention Type: DRUG

Other Intervention Names

ALDACTONE

Eligibility Criteria

Inclusion Criteria

• Men having an age ranging between 18 and 35 years
• Unhurt subject of any acute pathology for more than 7 days
• Consent signed by the subject

• Men or women having an age ranging between 18 and 85 years
• Unhurt subject of any acute pathology for more than 7 days
• without any cardiovascular treatment
• Consent signed by the subject

• Men or women having an age ranging between 18 and 85 years
• Subject with chronique renal insufficiency
• Consent signed by the subject

• Men or women having an age ranging between 30 and 60 years
• Hypertensive subject suffering from primary hyperaldosteronism defined by an aldosteron/renin ratio > 64 pmol/mL in position lying and an aldosteron level > 500pmol lying down (or > 550pmol/L seated or standing) or urinary aldosteron level > 63 nmol/24 hours; for whom
• Subjects having an indication for a monotherapy treatment by ALDACTONE (1 or 2 mg/kg/j)during preoperative period, possibly associated if need be with a calcic inhibitive treatment, central or alphabloquant treatment.
• Subject without other cardiovascular history (myocardic infarction, decompensation of cardiac insufficiency, intellectual vascular accident) and with a normal renal function according to the age,the sex and the blood value of creatinine by the formula MDRD (Flow of renal filtration > 60mL/min/1,73)
• Consent signed by the subject

Exclusion Criteria

• Subject presenting a known arterial high blood pressure (blood pressure > 140/90 mmHg) or any cardiovascular history
• Subject presenting a known renal insufficiency
• Subject presenting a known anaemia (Hb < 12g/dl)
• Subject presenting a known diabetes
• Subject presenting a cancer diagnosed less than 5 years previously or evolutionary cancer
• Subject presenting a chronic liver disease
• Subject presenting a connectivite: Rhumatoid polyarthritis....
• Crohns disease
• Current tuberculosis
• Any medicinal treatment
• Subject participating in another protocol or in exclusion's period for another protocol
• Absence of social insurance

For group 3

• Subject presenting a known arterial high blood pressure (blood pressure > 140/90 mmHg or > 160/95 mmHg after 60 years)
• Subject presenting a known renal insufficiency
• Subject presenting a known diabetes
• Woman who is pregnant
• Subject presenting a cancer diagnosed less than 5 years previously or evolutionary cancer
• Subject presenting a chronic liver disease
• Subject presenting a connectivite: Rhumatoid polyarthritis....
• Crohns disease
• Current tuberculosis
• Subject presenting an effort angor or other coronaropathy
• Subject with antecedant of endarterectomy of carotid
• Subject with known abdominal aorta aneurism
• Subject participating in another protocol or in exclusion's period for another protocol
• Absence of social insurance

For group 4

• Subject presenting a known diabetes
• Woman who is pregnant
• Subject presenting a cancer diagnosed less than 5 years previously or evolutionary cancer
• Subject presenting a chronic liver disease
• Subject presenting a connectivite: Rhumatoid polyarthritis....
• Crohns disease
• Current tuberculosis
• Subject presenting an effort angor or other coronaropathy
• Subject with antecedant of endarterectomy of carotid
• Subject with known abdominal aorta aneurism
• Subject participating in another protocol or in exclusion's period for another protocol
• Absence of social insurance

For the group 5

• Subject presenting others forms of high blood pressure (consumption of products or medicines which can facilitate or deteriorate the high blood pressure (alcohol, licorise (of whom the "pastis" without alcohol), anti-inflammatory drugs, corticosteroids, ciclosporin, erythropoiétin, cocain), hyperthyroidy, hypercorticism, chronic renal disease: urinary protein level < 0.3 g/l and clearance of the creatinine > 60ml/min/1.73m2), arterial renal stenosis
• Counter -indication of realization of a test of salt injection
• Current pregnancy
• Subject presenting a cancer diagnosed less than 5 years previously or evolutionary cancer
• Chronic liver disease
• Subject presenting a connectivite: Rheumatoid polyarthritis....
• Crohns disease
• Current tuberculosis
• Absence of social insurance

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 85 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Central Hospital, Nancy, France

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Michel AZIZI, Pr

Role: PRINCIPAL_INVESTIGATOR

Georges Pompidou Hospital, Paris

Locations

Brabois Hospital

Nancy, , France

Georges Pompidou Hospital

Paris, , France

Unit of arterial high blood pressure of the Georges Pompidou Hospital

Paris, , France

Countries

France

Other Identifiers

2007-A01008-45

Identifier Type: -

Identifier Source: org_study_id