Mechanisms of Insulin Resistance in Critical Illness: Role of Systemic Inflammation and GLP-1

NCT ID: NCT01347801

Last Updated: 2014-09-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

40 participants

Study Classification

INTERVENTIONAL

Study Start Date

2011-03-31

Study Completion Date

2014-09-30

Brief Summary

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The purpose of this study is to determine the role of inflammation and the insulin regulating hormone GLP-1 during critical illness.

Detailed Description

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Critically ill patients often exhibit hyperglycaemia. Although the cause of this hyperglycaemia is probably multifactorial, peripheral insulin resistance is a major contributor, similar to type 2 diabetes mellitus (T2D). There are several similarities between critical illness and T2D, including the presence of systemic inflammation and increased plasma free fatty acids (FFA), all of which may induce insulin resistance in healthy volunteers. In critical illness, elevated catecholamines, cortisol, growth hormone and glucagon may also contribute to insulin resistance.

The degree of hyperglycaemia correlates with mortality in ICU patients. van den Berghe et al. found that IV infusion of insulin to obtain strict normoglycaemia reduced mortality as well as morbidity in critically ill surgical patients and in some medical ICU patients.

However, insulin increases the risk of hypoglycaemia; this is a major obstacle to strict euglycaemia in ICU patients and may explain the inability of others to reproduce the benefits reported by van den Berghe et al. Thus, alternatives to insulin for controlling plasma glucose (PG) in ICU patients are warranted.

Aim:

To study the role of the incretin hormone, glucagon-like peptide (GLP)-1 for glycaemic, metabolic, hormonal and inflammatory profile in

* critically ill patients in the intensive care unit (ICU) and
* healthy volunteers exposed to a standardised systemic inflammation

Conditions

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Hypoglycaemia

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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2C - 1

TNF and OGTT and saline

Group Type PLACEBO_COMPARATOR

Placebo (Saline)

Intervention Type DRUG

Normal saline (NaCl 0,9%)

TNF-alfa

Intervention Type DRUG

1000ng/m2 BSA/hour i.v. infusion for 4-6 hours

OGTT

Intervention Type OTHER

Oral glucose tolerance test with 75 g glucose

2C - 2

TNF and OGTT and GLP-1

Group Type ACTIVE_COMPARATOR

GLP-1

Intervention Type DRUG

GLP-1 1,2pmol/kg/min i.v. infusion for 4 hours

TNF-alfa

Intervention Type DRUG

1000ng/m2 BSA/hour i.v. infusion for 4-6 hours

OGTT

Intervention Type OTHER

Oral glucose tolerance test with 75 g glucose

2C - 3

TNF and IVGTT and saline

Group Type PLACEBO_COMPARATOR

Placebo (Saline)

Intervention Type DRUG

Normal saline (NaCl 0,9%)

TNF-alfa

Intervention Type DRUG

1000ng/m2 BSA/hour i.v. infusion for 4-6 hours

IVGTT

Intervention Type OTHER

Intravenous glucose tolerance test with infusion of 20% glucose matching the glucose profile of the corresponding OGTT

2C - 4

TNF and IVGTT and GLP-1

Group Type ACTIVE_COMPARATOR

GLP-1

Intervention Type DRUG

GLP-1 1,2pmol/kg/min i.v. infusion for 4 hours

TNF-alfa

Intervention Type DRUG

1000ng/m2 BSA/hour i.v. infusion for 4-6 hours

IVGTT

Intervention Type OTHER

Intravenous glucose tolerance test with infusion of 20% glucose matching the glucose profile of the corresponding OGTT

2A-1

Saline infusion and OGTT

Group Type PLACEBO_COMPARATOR

Placebo (Saline)

Intervention Type DRUG

Normal saline (NaCl 0,9%)

OGTT

Intervention Type OTHER

Oral glucose tolerance test with 75 g glucose

2A-2

Saline and IVGTT

Group Type PLACEBO_COMPARATOR

Placebo (Saline)

Intervention Type DRUG

Normal saline (NaCl 0,9%)

IVGTT

Intervention Type OTHER

Intravenous glucose tolerance test with infusion of 20% glucose matching the glucose profile of the corresponding OGTT

2A-3

TNF and OGTT

Group Type ACTIVE_COMPARATOR

TNF-alfa

Intervention Type DRUG

1000ng/m2 BSA/hour i.v. infusion for 4-6 hours

OGTT

Intervention Type OTHER

Oral glucose tolerance test with 75 g glucose

2A-4

TNF and IVGTT

Group Type ACTIVE_COMPARATOR

TNF-alfa

Intervention Type DRUG

1000ng/m2 BSA/hour i.v. infusion for 4-6 hours

IVGTT

Intervention Type OTHER

Intravenous glucose tolerance test with infusion of 20% glucose matching the glucose profile of the corresponding OGTT

1C

OGTT and corresponding IVGTT

Group Type EXPERIMENTAL

OGTT

Intervention Type OTHER

Oral glucose tolerance test with 75 g glucose

IVGTT

Intervention Type OTHER

Intravenous glucose tolerance test with infusion of 20% glucose matching the glucose profile of the corresponding OGTT

Interventions

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GLP-1

GLP-1 1,2pmol/kg/min i.v. infusion for 4 hours

Intervention Type DRUG

Placebo (Saline)

Normal saline (NaCl 0,9%)

Intervention Type DRUG

TNF-alfa

1000ng/m2 BSA/hour i.v. infusion for 4-6 hours

Intervention Type DRUG

OGTT

Oral glucose tolerance test with 75 g glucose

Intervention Type OTHER

IVGTT

Intravenous glucose tolerance test with infusion of 20% glucose matching the glucose profile of the corresponding OGTT

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Healthy (assessed by medical history and clinical examination)
* Age 18-40years
* BMI \< 30kg/m2


* Age\>18 years
* HbA1C\<6,5%
* Admission to the ICU within the last 72 hours

Exclusion Criteria

* Previous resection of the small intestine (not including the appendix)
* presence of any inflammatory illness during the fortnight preceding the study
Minimum Eligible Age

18 Years

Maximum Eligible Age

40 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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University of Copenhagen

OTHER

Sponsor Role collaborator

Novo Nordisk A/S

INDUSTRY

Sponsor Role collaborator

Rigshospitalet, Denmark

OTHER

Sponsor Role lead

Responsible Party

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Kirsten Moller

MD, PH.D, DMSc

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Kirsten Møller, MD, Ph.D., DMSc

Role: PRINCIPAL_INVESTIGATOR

Centre of Inflammation and Metabolism

Locations

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Centre of Inflammation and Metabolism - Rigshospitalet 7641

Copenhagen, , Denmark

Site Status

University of Copenhagen

Copenhagen, , Denmark

Site Status

Countries

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Denmark

References

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Other Identifiers

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HS:H-3-2009-108

Identifier Type: -

Identifier Source: org_study_id

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