Study of Resting and Exercising Body Functioning in Freeman-Sheldon Syndrome and Related Conditions

NCT ID: NCT01306994

Last Updated: 2022-06-06

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

WITHDRAWN

Study Classification

OBSERVATIONAL

Study Start Date

2014-03-31

Study Completion Date

2022-06-30

Brief Summary

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The hypotheses of the present study of Freeman-Sheldon syndrome (FSS) and related conditions are: (1) that exercise capacity is lower in FSS patients versus normal controls, and the lower exercise capacity is due to changes in the muscles' normal structure and an inability of sufficient quantity of the energy molecule to bind to muscle; (2) this muscle problem reduces amount of air that can get in the lung and amount of oxygen carried in the blood, which then has the effect of increasing heart and respiration rates, blood pressure, and deep body temperature, and produces muscle rigidity; (3) the events noted above, when they occur during cardiac stress testing, are related to a problem similar to malignant hyperthermia (MH) reported in some muscle disorders without use of drugs known to cause MH. MH (a life-threatening metabolic reaction that classically is triggered when susceptible persons receive certain drugs used in anaesthesia.

Detailed Description

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This study is a research project initiated by the graduate research student (Mikaela I. Poling) and assisted by the clinical genetics fellow and graduate student (Andrés Morales) in partial fulfilment the requirements for their Masters degrees in Clinical and Applied Physiology, under approval, direction, and supervision of the study PI (Rodger J. McCormick).

Importance of Present Study:

FSS is a rare human neuromusculoskeletal disorder present before birth, involving primarily limb and craniofacial deformities. There are no prospective studies addressing physiological parameters, which are necessary to enable understanding of the underlying pathology and pathophysiology of Freeman-Sheldon syndrome. Elucidating any deviations in baseline and stress physiological parameters in FSS patients versus standard normal values and normal control subjects is of critical importance in tailoring therapeutic interventions to this challenging patient population.

Background:

Vanek et al. (1986) purposed FSS spectrum is a non-progressive congenital myopathy, giving pathological and electromyographical (EMG) evidence. They found white fibrose tissue within histologically normal muscle fibres, resulting in abnormal EMGs.

Toydemir et al. (2006) showed that mutations in embryonic myosin heavy chain 3 (MYH3), caused classic FSS phenotype, in which they screened 28 probands. In 20 patients, new missense mutations caused substitution of arginine at position 672 (arg672) by histidine or cytosine; arg672 is found in all myosin proteins post-embryonically. Of the remaining six patients in whom mutations were found, three had new missense or familial mutations; three other patients with sporadic expression had new, which were also found in Sheldon-Hall syndrome (SHS); two patients had no recognized mutations. They postulated these allelic variations at arg672 could affect adenosine triphosphate (ATP) binding. It is unknown how these mutations might correlate to the phenotypes observed. Their laboratory, including Stevenson et al. (2006) also presented strong evidence that FSS and SHS and similar distal arthrogryposes (DA) were distinct entities based on phenotype, natural history, and genotype.

Portillo et al. (unpublished data), in study of biopsies from their patient, found no evidence of muscle in the superior orbicularis oculi and found highly variable fibre size as a single pathological feature in a single vastus lateralis biopsy. Clinically, their patient, who had to-date the most severe expression of FSS, exhibited no function of the superior eyelid and reasonable muscle tone, bulk, and strength in the thigh. These findings suggested variable syndromic affectation by body region. They reported exertional dyspnea and resting tachycardia, without pathological features, in their patient and anecdotal information concerning exertional dyspnea in two other adult FSS patients. They also documented the occurrence of unexplained, seemingly stress-induced, episodic fever in their patient that resembled the malignant hyperthermia (MH) clinical triad of hyperthermia, tachycardia, and muscle rigidity.

In addition to age, gender, physical activity status, and concomitant disease and disability, maximal oxygen uptake, a function of exercise capacity, is genetically-controlled, and as already documented in other muscle disorders, the idiopathic febrile episodes reported by Portillo et al. may share physiological and biochemical similarities to the well-defined congenital muscle anomaly MH, which classically occurs when susceptible individuals receive inhaled anaesthetics, such as ether and halothane, or depolarizing muscle relaxants during surgery. Together, these clinical observations suggested there may be some syndromic relationship to exercise capacity and development of MH-like febrile syndrome, and it will be important to demonstrate these findings in a controlled experimental setting.

Significant differences among the similar distal arthrogryposes (DAs) may exist, with respect to the above, and this will be important to define experimentally, as well. Data concerning baseline and stress physiology in FSS and similar DAs could help to further define the distinct DA phenotypes clinically similar to FSS, contributing to nosological classification of FSS and related entities. This study will include FSS, Sheldon-Hall syndrome, DA type 1, and DA type 3.

Conditions

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Arthrogryposis Craniofacial Abnormalities

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

CROSS_SECTIONAL

Study Groups

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Syndrome Group

Individuals with Freeman-Sheldon, Sheldon-Hall, distal arthrogryposis type 1, or distal arthrogryposis type 3

Lactate, Glucose, and Adenosine Triphosphate Blood Levels

Intervention Type OTHER

Completed during the clinical examination and exercise test by the researchers, lactate, glucose, and free and total adenosine triphosphate blood levels are determined at rest and during exercise.

Physiological Stress Test

Intervention Type PROCEDURE

During exercise, heart and lung function are monitored for changes caused by exercise, which increases the body's need for oxygen and puts extra demands on the heart. In this study, testing is done using a cycle ergometer and conducted according to the standardised exponential exercise protocol (STEEP).

Functional Enquiry Form

Intervention Type OTHER

Evaluated before clinical examination, it is a checklist of medical problems.

Strength, Joint ROM, Girth and Length Measurements

Intervention Type OTHER

Completed during the clinical examination by the researchers, it is a structured approach to evaluation of muscles, joints, arms, thighs, and legs.

Study Physical Examination

Intervention Type OTHER

Completed during the clinical examination by the researchers, it is a structured approach to a full physical examination (minus breasts, genitalia, or rectum).

Observational Gait Analysis

Intervention Type OTHER

Completed during the clinical examination by researchers, it is a structured approach to evaluation of a person's walking.

Mental Health Interview

Intervention Type OTHER

Completed during the clinical examination by the researchers, it is a general evaluation of mental health status.

Control Group

Healthy individuals

Lactate, Glucose, and Adenosine Triphosphate Blood Levels

Intervention Type OTHER

Completed during the clinical examination and exercise test by the researchers, lactate, glucose, and free and total adenosine triphosphate blood levels are determined at rest and during exercise.

Physiological Stress Test

Intervention Type PROCEDURE

During exercise, heart and lung function are monitored for changes caused by exercise, which increases the body's need for oxygen and puts extra demands on the heart. In this study, testing is done using a cycle ergometer and conducted according to the standardised exponential exercise protocol (STEEP).

Functional Enquiry Form

Intervention Type OTHER

Evaluated before clinical examination, it is a checklist of medical problems.

Strength, Joint ROM, Girth and Length Measurements

Intervention Type OTHER

Completed during the clinical examination by the researchers, it is a structured approach to evaluation of muscles, joints, arms, thighs, and legs.

Study Physical Examination

Intervention Type OTHER

Completed during the clinical examination by the researchers, it is a structured approach to a full physical examination (minus breasts, genitalia, or rectum).

Observational Gait Analysis

Intervention Type OTHER

Completed during the clinical examination by researchers, it is a structured approach to evaluation of a person's walking.

Mental Health Interview

Intervention Type OTHER

Completed during the clinical examination by the researchers, it is a general evaluation of mental health status.

Interventions

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Lactate, Glucose, and Adenosine Triphosphate Blood Levels

Completed during the clinical examination and exercise test by the researchers, lactate, glucose, and free and total adenosine triphosphate blood levels are determined at rest and during exercise.

Intervention Type OTHER

Physiological Stress Test

During exercise, heart and lung function are monitored for changes caused by exercise, which increases the body's need for oxygen and puts extra demands on the heart. In this study, testing is done using a cycle ergometer and conducted according to the standardised exponential exercise protocol (STEEP).

Intervention Type PROCEDURE

Functional Enquiry Form

Evaluated before clinical examination, it is a checklist of medical problems.

Intervention Type OTHER

Strength, Joint ROM, Girth and Length Measurements

Completed during the clinical examination by the researchers, it is a structured approach to evaluation of muscles, joints, arms, thighs, and legs.

Intervention Type OTHER

Study Physical Examination

Completed during the clinical examination by the researchers, it is a structured approach to a full physical examination (minus breasts, genitalia, or rectum).

Intervention Type OTHER

Observational Gait Analysis

Completed during the clinical examination by researchers, it is a structured approach to evaluation of a person's walking.

Intervention Type OTHER

Mental Health Interview

Completed during the clinical examination by the researchers, it is a general evaluation of mental health status.

Intervention Type OTHER

Other Intervention Names

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ATP levels ATP blood levels adenosine triphosphate test blood test for ATP blood test for adenosine triphosphate ATP blood test adenosine triphosphate blood test ATP test glucose test glucose blood test sugar test lactic acid levels lactic acid blood test lactate blood test lactate levels stress test graded exercise test maximal exercise test VO2 max test maximal oxygen uptake test exercise tolerance test exercise stress test FSRG Form 08 health history review of systems systems review medical history personal health history SF 527 Standard Form 527 musculoskeletal examination musculoskeletal exam musculoskeletal evaluation musculoskeletal assessment extremity examination extremity evaluation extremity assessment extremity exam FSRG Form 14 physical examination physical evaluation physical assessment medical examination medical evaluation medical assessment full medical examination full medical evaluation full medical assessment periodic medical examination periodic medical evaluation periodic medical assessment annual medical examination annual medical evaluation annual medical assessment yearly medical examination yearly medical evaluation yearly medical assessment full physical examination full physical evaluation full physical assessment complete physical examination complete physical evaluation complete physical assessment yearly physical examination yearly physical evaluation yearly physical assessment annual physical examination annual physical evaluation annual physical assessment periodic physical examination periodic physical evaluation periodic physical assessment screening physical examination screening physical evaluation screening physical assessment screening physical physical periodic physical annual physical annual check-up annual check up yearly physical yearly check up yearly check-up check up check-up medical check-up medical check up annual medical check-up annual medical check up yearly medical check-up yearly medical check up periodic medical check-up periodic medical check up periodic health evaluation periodic health assessment annual health evaluation annual health assessment yearly health evaluation yearly health assessment screening health evaluation screening health assessment screening medical examination screening medical evaluation screening medical assessment med check FSRG 10 gait analysis functional gait analysis psychiatric examination psychiatric evaluation psychiatric assessment psychiatric interview diagnostic mental health examination diagnostic mental health evaluation diagnostic mental health assessment diagnostic psychiatric examination diagnostic psychiatric evaluation diagnostic psychiatric assessment diagnostic psychiatric interview intake examination intake evaluation intake assessment intake interview mental health evaluation mental health examination mental health assessment psychological examination psychological evaluation psychological assessment psychological interview psychological intake examination psychological intake evaluation psychological intake assessment psychological intake interview

Eligibility Criteria

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Inclusion Criteria

* Freeman-Sheldon syndrome,
* Sheldon-Hall syndrome,
* Distal arthrogryposis type 1, or
* Distal arthrogryposis type 3
* Deceased patients with enough clinical information available to satisfy study requirements


* Subjects must be healthy and free of active disease.
* Subject or parent of minor child must be willing to give consent.
* Subjects must fall within the age-bracket to be matched with a Syndrome Group patient already screened and enroled in the study
* Subjects must be non-tobacco users and non-drinkers.

Exclusion Criteria

* Individuals not confirmed to have a condition under study
* Deceased patients without enough clinical information available to satisfy study requirements
* Patients with other anomalies, not having one of the above syndromes
* Patients or parents of minor children not willing to give consent
* Mature female patients who are pregnant or breast-feeding will be reassessed for consideration for enrolment.
* Mature female patients who are currently experiencing menses will be reassessed for consideration for enrolment.
* Patients with active, acute comorbid illness will be reassessed for consideration for enrolment.


* Subjects exceptional for their age in body weight, stature, or habitus according to commonly accepted guidelines
* Subjects with active psychiatric illness, as manifested by abnormal mental status examination
* Subjects with active physical illness, especially respiratory or cardiac problem, as manifested by abnormal findings on physical examination
* Subjects with significant diagnosis of a constitutional disease or genetic disorder
* Subjects with a history of severe trauma resulting in either an anatomical of physiological deformity that impairs function
* Non-living subjects
* Candidates who fail the stress test
* Mature female subjects who are pregnant or breast-feeding will be reassessed for consideration for enrolment.
* Mature female subjects who are currently experiencing menses will be reassessed for consideration for enrolment.
* Subjects with active, acute illness will be reassessed for consideration for enrolment.
* Any other condition or anomaly expected to affect current physiology listed in AFI-48-123.
Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Freeman-Sheldon Research Group, Inc.

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Freeman-Sheldon Research Group, Inc. Headquarters

Buckhannon, West Virginia, United States

Site Status

Countries

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United States

References

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Vanek J, Janda J, Amblerova V, Losan F. Freeman-Sheldon syndrome: a disorder of congenital myopathic origin? J Med Genet. 1986 Jun;23(3):231-6. doi: 10.1136/jmg.23.3.231.

Reference Type BACKGROUND
PMID: 3723551 (View on PubMed)

Toydemir RM, Rutherford A, Whitby FG, Jorde LB, Carey JC, Bamshad MJ. Mutations in embryonic myosin heavy chain (MYH3) cause Freeman-Sheldon syndrome and Sheldon-Hall syndrome. Nat Genet. 2006 May;38(5):561-5. doi: 10.1038/ng1775. Epub 2006 Apr 16.

Reference Type BACKGROUND
PMID: 16642020 (View on PubMed)

Stevenson DA, Carey JC, Palumbos J, Rutherford A, Dolcourt J, Bamshad MJ. Clinical characteristics and natural history of Freeman-Sheldon syndrome. Pediatrics. 2006 Mar;117(3):754-62. doi: 10.1542/peds.2005-1219.

Reference Type BACKGROUND
PMID: 16510655 (View on PubMed)

Portillo AL, Chamberlain RL, McCormick RJ, Poling MI. Histopathological and Operative Findings in a Severe Case of Freeman-Sheldon Syndrome: Implications for Nosology and Therapy. (Unpubl.) 2010.

Reference Type BACKGROUND

Litman RS, Rosenberg H. Malignant hyperthermia: update on susceptibility testing. JAMA. 2005 Jun 15;293(23):2918-24. doi: 10.1001/jama.293.23.2918.

Reference Type BACKGROUND
PMID: 15956637 (View on PubMed)

McCormick RJ. A Proposal for a Thesis: Heat Tolerance in Exercising Lean and Obese Middle-Aged Men. DEd diss., the Pennsylvania State University, 1973.

Reference Type BACKGROUND

Myhill S, Booth NE, McLaren-Howard J. Chronic fatigue syndrome and mitochondrial dysfunction. Int J Clin Exp Med. 2009;2(1):1-16. Epub 2009 Jan 15.

Reference Type BACKGROUND
PMID: 19436827 (View on PubMed)

Northridge DB, Grant S, Ford I, Christie J, McLenachan J, Connelly D, McMurray J, Ray S, Henderson E, Dargie HJ. Novel exercise protocol suitable for use on a treadmill or a bicycle ergometer. Br Heart J. 1990 Nov;64(5):313-6. doi: 10.1136/hrt.64.5.313.

Reference Type BACKGROUND
PMID: 2245110 (View on PubMed)

Franklin B, Fern A, Fowler A, Spring T, Dejong A. Exercise physiologist's role in clinical practice. Br J Sports Med. 2009 Feb;43(2):93-8. doi: 10.1136/bjsm.2008.055202. Epub 2008 Dec 2.

Reference Type BACKGROUND
PMID: 19050005 (View on PubMed)

Other Identifiers

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U1111-1120-5931

Identifier Type: OTHER

Identifier Source: secondary_id

000079

Identifier Type: -

Identifier Source: org_study_id

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