Phase IIb Study of MP4OX in Traumatic Hemorrhagic Shock Patients

NCT ID: NCT01262196

Last Updated: 2013-08-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE2

Total Enrollment

348 participants

Study Classification

INTERVENTIONAL

Study Start Date

2011-05-31

Study Completion Date

2012-11-30

Brief Summary

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MP4OX is a novel oxygen therapeutic agent being developed as an ischemic rescue therapy to enhance perfusion and oxygenation of tissues at risk during hemorrhagic shock. MP4OX is a pegylated hemoglobin-based colloid. Due to its molecular size and unique oxygen dissociation characteristics, MP4OX targets delivery of oxygen to ischemic tissues. This study will evaluate the safety and efficacy of MP4OX treatment in trauma patients suffering from lactic acidosis due to severe hemorrhagic shock. The study hypothesis is that MP4OX will reverse the lactic acidosis by enhancing perfusion and oxygenation of ischemic tissues and thereby prevent and reduce the duration of organ failure and improve outcome in these patients.

Detailed Description

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Acute traumatic injury, including both blunt and penetrating injury, is often associated with severe uncontrolled bleeding which can lead to hemorrhagic shock. During shock, inadequate blood flow results in local ischemia and tissue hypoxia (insufficient oxygenation) of critical organs, which can be detected by an increase in serum lactate levels in these trauma victims. Despite optimal care, more than 10% of trauma victims who reach hospital alive will die, and many will suffer from organ failure. Death and significant, persistent morbidity are consequences of trauma, and traumatic injuries are associated with lost productivity, reduced quality of life, and direct costs to patients and health care systems worldwide.

The primary treatment of trauma is to support ventilation and oxygenation, limit blood loss, and maintain cardiovascular function so that organs are perfused. The patient's airway may be intubated to allow oxygenated airflow to the lungs. Mechanical ventilation is used if the patient cannot maintain oxygenation and carbon dioxide elimination. Damage-control surgery is used to limit blood loss and to intentionally delay definitive repair until the patient can better tolerate procedures. Blood transfusions are provided to maintain the oxygen-carrying capacity of the circulation. Platelets and coagulation factors are infused to correct any coagulopathy from dilution of blood and consumption of clotting factors. Vasopressor and inotropic agents may be used to support low cardiac output or blood pressure. Renal replacement therapy may be instituted if kidney failure occurs.

Despite optimal care, organ dysfunction is present in many patients. Hypoperfusion and anaerobic metabolism of organs and tissues can be detected by the presence of lactic acidosis. Current therapy is aimed at supporting failing organs, but an agent that accelerates the repayment of an oxygen debt and prevents or shortens the duration of organ failure is sought. Blood transfusion improves circulation of oxygen-carrying red blood cells but is insufficient if lactic acidosis is present, even when the hemoglobin level has been restored. Studies in critically ill intensive care patients have demonstrated that elevated initial and 24-hour lactate levels are significantly correlated with mortality, and prolonged elevation of blood lactate levels after trauma has been correlated with increased organ failure and mortality.

Support for the efficacy of MP4OX in resuscitation of severe hemorrhage shock comes from several preclinical studies in hamster, rat, and swine. Using a swine model of uncontrolled hemorrhage and resuscitation, survival was greater and restoration of hemodynamics and acid-base status were improved with MP4OX relative to equivalent volume of crystalloid, pentastarch, or unmodified hemoglobin. Administration of MP4OX improved 24-hour survival, stabilized cardiac output and arterial pressure at nearly normal levels, and reduced lactate more effectively than control fluids. Importantly, these benefits of MP4OX were observed with or without co-administration of autologous blood, suggesting that blood alone is not sufficient to achieve resuscitation, and that the effects of MP4OX are additional to those of blood.

Additional support comes from a recently completed phase IIa trauma study in 51 patients with lactic acidosis due to severe hemorrhage. MP4OX treatment was associated with a more rapid and sustained reduction of high lactate levels, and a greater proportion of MP4OX-treated patients who normalized lactate by four hours after dosing. There was also a trend toward shorter median hospital stay and a greater proportion of MP4OX-treated patients being discharged from hospital alive by Day 28. These phase IIa results suggest improved oxygen delivery and utilization by ischemic tissues in the MP4OX-treated patients, based on the reversal of lactic acidosis, and support the positive results from the preclinical models of hemorrhagic shock resuscitation.

Conditions

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Shock, Hemorrhagic Shock, Traumatic Acidosis, Lactic

Keywords

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Trauma Hemorrhage Hemorrhagic shock Lactic acidosis Oxygen carriers Oxygen therapeutics Hemoglobin solutions Red cell substitutes PEG-hemoglobin

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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MP4OX

250-mL dose

Group Type EXPERIMENTAL

MP4OX

Intervention Type DRUG

4.3 g/dL pegylated hemoglobin in balanced lactate-electrolyte solution

Control

250-mL of normal saline solution

Group Type PLACEBO_COMPARATOR

Saline

Intervention Type DRUG

Normal saline (0.9%) solution

Interventions

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MP4OX

4.3 g/dL pegylated hemoglobin in balanced lactate-electrolyte solution

Intervention Type DRUG

Saline

Normal saline (0.9%) solution

Intervention Type DRUG

Other Intervention Names

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Hemoglobin pegylated MalPEG-Hb MP4 PEG-Hb Pegylated-Hb Normal saline Saline solution Sodium chloride 0.9%

Eligibility Criteria

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Inclusion Criteria

* Adult male or female (surgically sterile or post-menopausal or confirmed not to be pregnant)
* Trauma injury (blunt and/or penetrating) resulting in lactic acidosis due to hemorrhagic shock
* Acidosis (blood lactate level ≥ 5 mmol/L; equivalent to 45 mg/dL) arterial or venous

Exclusion Criteria

* Massive injury incompatible with life
* Normalization of lactate prior to dosing (≤ 2.2 mmol/L)
* Patients with evidence of severe traumatic brain injury as defined by ANY one of the following: Known non-survivable head injury or open brain injury; Glasgow Coma Score (GCS) = 3, 4 or 5; Known AIS (head region) ≥ 4 shown by an appropriate imaging methodology; Contemplated CNS surgery; or Abnormal physical exam indicative of severe CNS or any spinal cord injury above T5 level
* Cardiac arrest prior to randomization
* Age below the legal age for consenting
* Estimated time from injury to randomization\> 4 hours
* Estimated time from hospital admission to randomization \> 2 hours
* Known pregnancy
* Use of any oxygen carrier other than RBCs
* Known previous participation in this study
* Professional or ancillary personnel involved with this study
* Known receipt of any investigational drug(s) within 30 days prior to study
Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Sangart

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Karim Brohi, MD

Role: PRINCIPAL_INVESTIGATOR

The Royal London Hospital

Locations

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Liverpoool Hospital NSW

Liverpool, , Australia

Site Status

John Hunter Hospital

Newcastle, , Australia

Site Status

Graz University Hospital

Gratz, , Austria

Site Status

Faculdade de Medicina de S. J. Do Rio Preto

São José do Rio Preto, , Brazil

Site Status

Hospital das Clínicas - USP

São Paulo, , Brazil

Site Status

Hospital Universitário - USP Ribeirão Preto

São Paulo, , Brazil

Site Status

Fundacion Valle de Lili

Cali, , Colombia

Site Status

Hôpital Beaujon

Clichy, , France

Site Status

Hôpital Michallon

Grenoble, , France

Site Status

Hôpital du Kremlin Bicêtre

Le Kremlin-Bicêtre, , France

Site Status

Hôpital Roger Salengro, CHRU Lille

Lille, , France

Site Status

Hôpital Dupuytren, CHU Limoges

Limoges, , France

Site Status

Hôpital Edouard Herriot

Lyon, , France

Site Status

Hôpital Lyon sud

Lyon, , France

Site Status

Hôpital Pitié-Salpêtrière

Paris, , France

Site Status

Universitätsklinikum der RWTH Aachen

Aachen, , Germany

Site Status

Charite - Campus Virchow Klinikum

Berlin, , Germany

Site Status

Kliniken der Stadt Köln Merheim

Cologne, , Germany

Site Status

Klinikum der Johann-Wolfgang-Goethe-Universität Frankfurt a.M.

Frankfurt, , Germany

Site Status

BG Klinik Ludwigshafen

Ludwigshafen, , Germany

Site Status

Soroka University Medical Center

Beersheba, , Israel

Site Status

Rambam Hospital

Haifa, , Israel

Site Status

Hadassah Medical Center

Jerusalem, , Israel

Site Status

Auckland Hospital

Auckland, , New Zealand

Site Status

Oslo university hospital

Oslo, , Norway

Site Status

National University Hospital

Singapore, , Singapore

Site Status

Singapore General Hospital

Singapore, , Singapore

Site Status

Tan Tock Seng Hospital

Singapore, , Singapore

Site Status

Netcare Union Hospital

Alberton, , South Africa

Site Status

Vincent Pallotti Hospital

Cape Town, , South Africa

Site Status

Netcare Unitas Hospital

Centurion, , South Africa

Site Status

Charlotte Maxeke Johannesburg Hospital

Johannesburg, , South Africa

Site Status

Netcare Milpark Hospital

Johannesburg, , South Africa

Site Status

Chris Baragwanath Hospital

Soweto, , South Africa

Site Status

Hospital 12 de Octubre, Madrid

Madrid, , Spain

Site Status

Centre Hospitalier Universitaire Vaudois CHUV

Lausanne, , Switzerland

Site Status

Universitätsspital Zürich

Zurich, , Switzerland

Site Status

King's College Hospital, London

London, , United Kingdom

Site Status

The Royal London Hospital

London, , United Kingdom

Site Status

John Radcliffe Hospital, Oxford

Oxford, , United Kingdom

Site Status

Countries

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Belgium Canada Finland Italy Australia Austria Brazil Colombia France Germany Israel New Zealand Norway Singapore South Africa Spain Switzerland United Kingdom

References

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Young MA, Lohman J, Malavalli A, Vandegriff KD, Winslow RM. Hemospan improves outcome in a model of perioperative hemodilution and blood loss in the rat: comparison with hydroxyethyl starch. J Cardiothorac Vasc Anesth. 2009 Jun;23(3):339-47. doi: 10.1053/j.jvca.2008.08.006. Epub 2008 Oct 22.

Reference Type BACKGROUND
PMID: 18948027 (View on PubMed)

Young MA, Riddez L, Kjellstrom BT, Winslow RM. Effect of maleimide-polyethylene glycol hemoglobin (MP4) on hemodynamics and acid-base status after uncontrolled hemorrhage in anesthetized swine: comparison with crystalloid and blood. J Trauma. 2007 Dec;63(6):1234-44. doi: 10.1097/TA.0b013e31815bd7b0.

Reference Type BACKGROUND
PMID: 18212644 (View on PubMed)

Young MA, Riddez L, Kjellstrom BT, Bursell J, Winslow F, Lohman J, Winslow RM. MalPEG-hemoglobin (MP4) improves hemodynamics, acid-base status, and survival after uncontrolled hemorrhage in anesthetized swine. Crit Care Med. 2005 Aug;33(8):1794-804. doi: 10.1097/01.ccm.0000172648.55309.13.

Reference Type BACKGROUND
PMID: 16096458 (View on PubMed)

Drobin D, Kjellstrom BT, Malm E, Malavalli A, Lohman J, Vandegriff KD, Young MA, Winslow RM. Hemodynamic response and oxygen transport in pigs resuscitated with maleimide-polyethylene glycol-modified hemoglobin (MP4). J Appl Physiol (1985). 2004 May;96(5):1843-53. doi: 10.1152/japplphysiol.00530.2003. Epub 2004 Jan 16.

Reference Type BACKGROUND
PMID: 14729723 (View on PubMed)

Vandegriff KD, Winslow RM. Hemospan: design principles for a new class of oxygen therapeutic. Artif Organs. 2009 Feb;33(2):133-8. doi: 10.1111/j.1525-1594.2008.00697.x.

Reference Type BACKGROUND
PMID: 19178457 (View on PubMed)

Vandegriff KD, Malavalli A, Mkrtchyan GM, Spann SN, Baker DA, Winslow RM. Sites of modification of hemospan, a poly(ethylene glycol)-modified human hemoglobin for use as an oxygen therapeutic. Bioconjug Chem. 2008 Nov 19;19(11):2163-70. doi: 10.1021/bc8002666.

Reference Type BACKGROUND
PMID: 18837531 (View on PubMed)

Svergun DI, Ekstrom F, Vandegriff KD, Malavalli A, Baker DA, Nilsson C, Winslow RM. Solution structure of poly(ethylene) glycol-conjugated hemoglobin revealed by small-angle X-ray scattering: implications for a new oxygen therapeutic. Biophys J. 2008 Jan 1;94(1):173-81. doi: 10.1529/biophysj.107.114314. Epub 2007 Sep 7.

Reference Type BACKGROUND
PMID: 17827244 (View on PubMed)

Winslow RM, Lohman J, Malavalli A, Vandegriff KD. Comparison of PEG-modified albumin and hemoglobin in extreme hemodilution in the rat. J Appl Physiol (1985). 2004 Oct;97(4):1527-34. doi: 10.1152/japplphysiol.00404.2004. Epub 2004 Jun 18.

Reference Type BACKGROUND
PMID: 15208289 (View on PubMed)

Tsai AG, Cabrales P, Manjula BN, Acharya SA, Winslow RM, Intaglietta M. Dissociation of local nitric oxide concentration and vasoconstriction in the presence of cell-free hemoglobin oxygen carriers. Blood. 2006 Nov 15;108(10):3603-10. doi: 10.1182/blood-2006-02-005272. Epub 2006 Jul 20.

Reference Type BACKGROUND
PMID: 16857991 (View on PubMed)

Tsai AG, Vandegriff KD, Intaglietta M, Winslow RM. Targeted O2 delivery by low-P50 hemoglobin: a new basis for O2 therapeutics. Am J Physiol Heart Circ Physiol. 2003 Oct;285(4):H1411-9. doi: 10.1152/ajpheart.00307.2003. Epub 2003 Jun 12.

Reference Type BACKGROUND
PMID: 12805024 (View on PubMed)

Olofsson CI, Gorecki AZ, Dirksen R, Kofranek I, Majewski JA, Mazurkiewicz T, Jahoda D, Fagrell B, Keipert PE, Hardiman YJ, Levy H; Study 6084 Clinical Investigators. Evaluation of MP4OX for prevention of perioperative hypotension in patients undergoing primary hip arthroplasty with spinal anesthesia: a randomized, double-blind, multicenter study. Anesthesiology. 2011 May;114(5):1048-63. doi: 10.1097/ALN.0b013e318215e198.

Reference Type BACKGROUND
PMID: 21455059 (View on PubMed)

van der Linden P, Gazdzik TS, Jahoda D, Heylen RJ, Skowronski JC, Pellar D, Kofranek I, Gorecki AZ, Fagrell B, Keipert PE, Hardiman YJ, Levy H; 6090 Study Investigators. A double-blind, randomized, multicenter study of MP4OX for treatment of perioperative hypotension in patients undergoing primary hip arthroplasty under spinal anesthesia. Anesth Analg. 2011 Apr;112(4):759-73. doi: 10.1213/ANE.0b013e31820c7b5f. Epub 2011 Feb 11.

Reference Type BACKGROUND
PMID: 21317165 (View on PubMed)

Related Links

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http://www.sangart.com

Sangart Inc. (San Diego, CA) web site

Other Identifiers

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TRA-205

Identifier Type: -

Identifier Source: org_study_id