Efficacy and Safety of Methylene Blue in the Treatment of Severe Septic Shock

NCT ID: NCT06481410

Last Updated: 2024-12-30

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

488 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-06-22

Study Completion Date

2026-06-20

Brief Summary

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The goal of this clinical trial is to investigate whether methylene blue injection can safely and effectively improve the survival rate of patients with severe septic shock, shorten the duration of norepinephrine use, reduce the dosage of vasopressors, promptly correct hemodynamics, and improve tissue perfusion and organ function impairment.

Detailed Description

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Researchers will compare methylene blue to a placebo (a look-alike substance that contains no drug) to see if methylene blue works to treat severe septic shock.

Participants will:

Administer a loading dose of 2.5 mg/kg via micro-pump over 15 minutes, followed by a maintenance infusion at a rate of 0.25 mg/kg/hour for 12 hours or until norepinephrine has been continuously discontinued for 4 hours, whichever comes first. If norepinephrine needs to be increased to 0.25 μg/kg/min before the 12-hour period ends, continue using methylene blue until the 12-hour infusion is completed.

Primary Study Endpoint

\- 28-day all-cause mortality rate starting from the diagnosis of septic shock.

Secondary Study Endpoints

* Time from the start to the discontinuation of norepinephrine after the diagnosis of septic shock.
* Total dose of norepinephrine used (from enrollment to 72 hours).
* Number of days without norepinephrine within 28 days.
* Duration of mechanical ventilation.
* Rate of CRRT (Continuous Renal Replacement Therapy).
* Length of ICU stay.
* Total hospital stay.

Record the following indicators before methylene blue intervention and at 24 hours, 72 hours, and 5 days after the intervention:

* Highest serum lactate level.
* Lowest mean arterial pressure.
* Alanine aminotransferase (ALT).
* Total bilirubin.
* Creatinine.
* Oxygenation index (P/F ratio).
* Creatine kinase-MB (CK-MB).
* Troponin I (TNI).
* Systemic vascular resistance index (SVRI).

Conditions

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Septic Shock Methylene Blue Sepsis

Keywords

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Septic Shock Methylene blue Sepsis

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

This is a prospective, randomized, controlled, single-blind study.
Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Outcome Assessors
This study implements blinding for the outcome assessor.

Study Groups

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Methylene Blue Intervention Group

Methylene blue injection, 2.5 mg/kg loading dose over 15 minutes, followed by a continuous infusion of 0.25 mg/kg/hour for 12 hours or until norepinephrine has been continuously discontinued for 4 hours, whichever comes first.

Group Type EXPERIMENTAL

Methylene Blue Intervention Group

Intervention Type DRUG

In addition to standard treatment, patients will receive methylene blue intervention. The method is as follows: a loading dose of 2.5 mg/kg administered via micro-pump over 15 minutes, followed by a continuous infusion at a rate of 0.25 mg/kg/hour for 12 hours or until norepinephrine has been continuously discontinued for 4 hours, whichever comes first. If norepinephrine needs to be increased to 0.1 μg/kg/min before the 12-hour period ends, continue using methylene blue until the 12-hour infusion is completed.

Normal Saline Control Group

Normal saline solution, administered in the same manner and duration as the methylene blue intervention.

Group Type PLACEBO_COMPARATOR

Normal Saline Control Group

Intervention Type DRUG

In addition to standard treatment, patients will receive a placebo control with normal saline, administered for the same duration as the intervention group.

Interventions

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Methylene Blue Intervention Group

In addition to standard treatment, patients will receive methylene blue intervention. The method is as follows: a loading dose of 2.5 mg/kg administered via micro-pump over 15 minutes, followed by a continuous infusion at a rate of 0.25 mg/kg/hour for 12 hours or until norepinephrine has been continuously discontinued for 4 hours, whichever comes first. If norepinephrine needs to be increased to 0.1 μg/kg/min before the 12-hour period ends, continue using methylene blue until the 12-hour infusion is completed.

Intervention Type DRUG

Normal Saline Control Group

In addition to standard treatment, patients will receive a placebo control with normal saline, administered for the same duration as the intervention group.

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

1. Age ≥ 18 years.
2. Meets the Sepsis 3.0 criteria for septic shock: patients with infection who, despite adequate fluid resuscitation, require vasopressor therapy to maintain a mean arterial pressure (MAP) of ≥ 65 mmHg and have a blood lactate concentration \> 2 mmol/L.
3. Diagnosed with septic shock and started on norepinephrine within 24 hours.
4. Requires a norepinephrine dose of ≥ 0.1 μg/kg/min to maintain a MAP of ≥ 65 mmHg.

Exclusion Criteria

1. Pregnant or breastfeeding women.
2. Individuals allergic to methylene blue or any components of the methylene blue injection.
3. Individuals with a personal or family history of glucose-6-phosphate dehydrogenase (G6PD) deficiency.
4. Patients with an expected survival time of less than 48 hours.
5. Patients who have been on norepinephrine for more than 24 hours.
Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Yuyao People's Hospital

OTHER

Sponsor Role collaborator

Taizhou Enze Medical Center Group

OTHER

Sponsor Role collaborator

NINGBO MEDICAL CENTER LIHUIIHOSPITAI

UNKNOWN

Sponsor Role collaborator

People's Hospital of Guangxi Zhuang Autonomous Region

OTHER

Sponsor Role collaborator

The second Nanning People's Hospital

UNKNOWN

Sponsor Role collaborator

GUI LIN PEOPLE'S HOSPITAL

UNKNOWN

Sponsor Role collaborator

Guangxi Hospital Division of The First Affiliated Hospital, Sun Yat-sen University

UNKNOWN

Sponsor Role collaborator

The First People's Hospital of Huzhou

OTHER

Sponsor Role collaborator

Hangzhou Yuhang District Second People's Hospital

UNKNOWN

Sponsor Role collaborator

Wuming Hospital of Guangxi Medical University

UNKNOWN

Sponsor Role collaborator

First Affiliated Hospital of Zhejiang University

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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The First Affiliated Hospital, Zhejiang University School of Medicine

Hangzhou, Zhejiang, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Wenqiao Yu, PhD

Role: CONTACT

Phone: +86-18868787588

Email: [email protected]

Facility Contacts

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Wenqiao Yu, PhD

Role: primary

Other Identifiers

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2024-051

Identifier Type: -

Identifier Source: org_study_id