Genetics of Obesity in Chinese Youngs

NCT ID: NCT01084967

Last Updated: 2021-04-21

Basic Information

• Recruitment Status: RECRUITING
• Total Enrollment: 8000 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2009-03-31
• Study Completion Date: 2026-04-30

Brief Summary

The purpose of this study is to explore the pathogenesis and genetic susceptibility of obese subjects,providing a convincing argument for further treatment of obesity and metabolic syndrome.

Detailed Description

Obesity has become a major worldwide challenge to public health, owing to an interaction between the obesogenic environment and a strong genetic contribution.Previous studies found that genetic factors determine 40%-70% of obese phenotype.Under such circumstances, the screening of obesity susceptibility gene is particularly important for society or family to take measures to prevent obesity.

Recent extensive genome-wide association studies(GWASs) have identified numerous single nucleotide polymorphisms associated with obesity,but these loci together account for only a small fraction of the known heritable component.Two studies in 2010 Nature,for the first time, put rare copy number variation (CNV)in association with severe early-onset obesity.Their significance lie not only in the discovery of the pathogenic genes of severe early-onset obesity but,more importantly,in providing new strategies for finding out genes that cause complex diseases.

Obese patients and healthy lean controls proved by a series of blood biochemical examinations will be enrolled in this study.The present study intends to use the techniques such as enzyme-linked immunosorbent assay, real-time fluorescence quantitative PCR,gene chip,construction of viral vectors,transfection, taking advantage of the established database,by means of serum assays and functional tests, associate copy number variation with obesity phenotype to explain the root cause of obesity.Meanwhile biomarkers, gut flora and genetic risk factors will be evaluated in the study subjects.

Conditions

Obesity; Metabolic Syndrome

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: CROSS_SECTIONAL

Study Groups

Healthy lean control group

The healthy lean controls consisted of age-, sex- and geography-matched young volunteers, or "golden standard" lean controls from China Cardiometabolic Disease and Cancer Cohort Study (4C Study).

Healthy volunteers:

1. BMI:18.5-22.9kg/m2;

2. Age:14-30 years old;

3. To be proved normal by the examinations of liver and kidney function,blood lipids profile,FBG, PBG, fasting insulin and HbA1c.

Controls from 4C Study:

1. 17.0≤BMI≤23.0kg/m2 and waist circumference <85cm for males and <80cm for females;

2. 1.8≤LDL-c≤3.4mmol/l;

3. FBG<6.1, PBG<7.8;

4. HOMA-IR<2.5;

5. BP <140/90mmHg;

6. eGFR>60mL/(min·1.73 m2), ALT<40IU/L, AST<40IU/L;

7. Excluding the history of hyperthyroidism, digestive system diseases, malignant tumors, infectious diseases (hepatitis B) and other systemic wasting diseases; Severe dysfunction of heart, lung and kidney;

8. Not using antihypertensive, hypoglycemic and lipid-lowering drugs；

9. No history of smoking.

No interventions assigned to this group

obesity group

1. BMI ≥30kg/m2;

2. Age:14-30 years old; obesity group 4000, lean healthy control group 4000

No interventions assigned to this group

Eligibility Criteria

Inclusion Criteria

• body mass index (BMI)≥ 30kg/m2
• predominantly east China to minimize population stratification
• willing and able to provide informed consent

Exclusion Criteria

• pregnancy/lactation
• Cushing syndrome
• Hypothyroidism
• obesity caused by pituitary and hypothalamic lesions
• drug related obesity
• history of major psychiatric illness

• Minimum Eligible Age: 14 Years
• Maximum Eligible Age: 30 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Shanghai Jiao Tong University School of Medicine

OTHER

Sponsor Role: lead

Responsible Party

Guang Ning

Director

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Guang Ning, MD.PhD

Role: STUDY_CHAIR

Shanghai Jiao Tong University School of Medicine

Jie Hong, MD

Role: PRINCIPAL_INVESTIGATOR

Shanghai Jiao Tong University School of Medicine

Jiqiu Wang, MD.PhD

Role: PRINCIPAL_INVESTIGATOR

Shanghai Jiao Tong University School of Medicine

Locations

Shanghai Jiao Tong University School of Medicine

Shanghai, Shanghai Municipality, China

Site Status: RECRUITING

Countries

China

Central Contacts

Guang Ning, MD.PhD

Role: CONTACT

guangning@medmail.com.cn

86-21-64370045 ext. 665340

Jie Hong, PhD

Role: CONTACT

hongjie13d@hotmail.com

86-21-64370045 ext. 665345

Facility Contacts

Guang Ning, MD.PhD

Role: primary

guangning@medmail.com.cn

86-21-64370045 ext. 665340

Jie Hong, PhD

Role: backup

hongjie13d@hotmail.com

86-21-64370045 ext. 665345

References

Hong J, Bo T, Xi L, Xu X, He N, Zhan Y, Li W, Liang P, Chen Y, Shi J, Li D, Yan F, Gu W, Wang W, Liu R, Wang J, Wang Z, Ning G. Reversal of Functional Brain Activity Related to Gut Microbiome and Hormones After VSG Surgery in Patients With Obesity. J Clin Endocrinol Metab. 2021 Aug 18;106(9):e3619-e3633. doi: 10.1210/clinem/dgab297.

Reference Type: DERIVED

PMID: 33950216 (View on PubMed)

Other Identifiers

CCEMD008

Identifier Type: -

Identifier Source: org_study_id