CXCL9, CXCL10, CXCL11, and CXCR3 in Dry Eye Syndrome

NCT ID: NCT00991679

Last Updated: 2009-10-08

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

48 participants

Study Classification

OBSERVATIONAL

Study Start Date

2008-02-29

Study Completion Date

2008-12-31

Brief Summary

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The aim of this study is to investigate the expression of CXCL9, CXCL10, CXCL11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.

Thirty-three patients with dry eye (16 Sjögren's syndrome and 17 non-Sjögren's syndrome patients) and 15 control subjects were recruited. The concentrations of CXCL9, CXCL10, and CXCL11 in tears were measured using enzyme-linked immunosorbent assay. The correlation between chemokine levels and tear film and ocular surface parameters was analyzed. Expression of CXCL9, CXCL10, CXCL11, and CXCR3 in the conjunctiva was evaluated using immunohistochemistry. Flow cytometry was performed to count CXCR3+ cells and CXCR3+CD4+ cells in the conjunctiva.

Detailed Description

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Conditions

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Dry Eye Syndrome

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Normal control subjects

Normal control subjects who did not show the clinical symptom and findings of dry eye syndrome.

No interventions assigned to this group

dry eye patients

Patients with dry eye syndrome who had symptoms of dry eye for more than 3 months, low tear film break up time (BUT, ≤7 sec), low Schirmer test (\<10 mm), low tear clearance rate (\<8X), and positive fluorescein or rose bengal vital staining (≥3) and were not treated with anti-inflammatory agents such as topical cyclosporine or steroids were included in the study.

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

* clinical diagnosis of dry eye syndrome

Exclusion Criteria

* active ocular infection or inflammation not associated with dry eye
* drug toxicity
* contact lens wear
* ocular allergy
* ocular surgery within the last 3 months
* lid or lash abnormalities
Minimum Eligible Age

24 Years

Maximum Eligible Age

77 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Chonnam National University Hospital

OTHER

Sponsor Role lead

Responsible Party

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Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, South Korea

Principal Investigators

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Kyung-Chul Yoon, MD, PhD

Role: STUDY_DIRECTOR

Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, South Korea

Locations

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Chonnam National University Hospital

Gwangju, Chonnam, South Korea

Site Status

Countries

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South Korea

References

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Yoon KC, De Paiva CS, Qi H, Chen Z, Farley WJ, Li DQ, Pflugfelder SC. Expression of Th-1 chemokines and chemokine receptors on the ocular surface of C57BL/6 mice: effects of desiccating stress. Invest Ophthalmol Vis Sci. 2007 Jun;48(6):2561-9. doi: 10.1167/iovs.07-0002.

Reference Type RESULT
PMID: 17525185 (View on PubMed)

Wallace GR, John Curnow S, Wloka K, Salmon M, Murray PI. The role of chemokines and their receptors in ocular disease. Prog Retin Eye Res. 2004 Jul;23(4):435-48. doi: 10.1016/j.preteyeres.2004.04.004.

Reference Type RESULT
PMID: 15219876 (View on PubMed)

Pflugfelder SC, Jones D, Ji Z, Afonso A, Monroy D. Altered cytokine balance in the tear fluid and conjunctiva of patients with Sjogren's syndrome keratoconjunctivitis sicca. Curr Eye Res. 1999 Sep;19(3):201-11. doi: 10.1076/ceyr.19.3.201.5309.

Reference Type RESULT
PMID: 10487957 (View on PubMed)

Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC. Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome. Invest Ophthalmol Vis Sci. 2010 Feb;51(2):643-50. doi: 10.1167/iovs.09-3425. Epub 2009 Oct 22.

Reference Type DERIVED
PMID: 19850844 (View on PubMed)

Other Identifiers

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I-2007-12-111

Identifier Type: -

Identifier Source: org_study_id

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