Exhaled Nitric Oxide and Nasal Lavage Fluid

NCT ID: NCT00773578

Last Updated: 2009-02-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

49 participants

Study Classification

OBSERVATIONAL

Study Start Date

2007-10-31

Study Completion Date

2009-02-28

Brief Summary

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The allergic respiratory diseases such as asthma or allergic rhinitis are the main chronic diseases in children. The airway inflammation plays an important role in their pathophysiology. Several epidemiological studies show that environmental tobacco smoke (ETS) exacerbates the symptoms of asthma and allergies.

The aim of this study is to assess the impact of environmental tobacco smoke on the measurement of exhaled nitric oxide (eNO) and biomarkers of nasal inflammation in atopic asthmatic children.

Detailed Description

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Type of study :

This study is a epidemiological observation study type exposed or unexposed to environmental tobacco smoke.

Methods :

The eNO is determined by a method On-line, at a rate expiratory 50 mL / s, using a chemiluminescence NO analyzer, before the allergy assessment (skin tests and assay of serum total and specific IgE ) and measurement of lung function (spirometry). Nasal lavage is performed by using a validated technique . Cells are counted and differentiated after Giemsa staining. We measured 1-anti-trypsin, neutrophil elastase, albumin, urea, IL4 , IL6, IL8 and IL13. Exposure to ETS is assessed by questionnaire to the parents. Statistical analysis include a narrative strand to verify the normality of distribution and an analytical analysis, including a bivariate followed by a multivariate analysis by multiple linear regression and / or logistic regression to model concentrations of biomarkers of inflammation and adjustment variables such as age, sex, atopic status, eosinophilia and severity of asthma.

Conditions

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Asthma

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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1

atopic asthmatic children exposed to environmental tobacco smoke

Measurement of exhaled nitric oxide (eNO)

Intervention Type DEVICE

Chemiluminescence NO analyzer Measurement of lung function (spirometry)

Measurement of biomarkers of nasal inflammation

Intervention Type DEVICE

Nasal lavage

2

atopic asthmatic children unexposed to environmental tobacco smoke

Measurement of exhaled nitric oxide (eNO)

Intervention Type DEVICE

Chemiluminescence NO analyzer Measurement of lung function (spirometry)

Measurement of biomarkers of nasal inflammation

Intervention Type DEVICE

Nasal lavage

Interventions

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Measurement of exhaled nitric oxide (eNO)

Chemiluminescence NO analyzer Measurement of lung function (spirometry)

Intervention Type DEVICE

Measurement of biomarkers of nasal inflammation

Nasal lavage

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

* Aged over 5 years
* Atopic, persistent mild and moderate Asthmatic Children (GINA 2 and 3)

Exclusion Criteria

* Child with asthma medication asthma used in the previous 15 days (oral or inhaled corticosteroids, leukotriene antagonist)
* Recent asthma attack (day of hospitalization or 15 days earlier)
* Upper respiratory infection (4 weeks) and low (2 weeks)
* Active smoking (teenager)
Minimum Eligible Age

5 Years

Maximum Eligible Age

16 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Assistance Publique - Hôpitaux de Paris

OTHER

Sponsor Role lead

Responsible Party

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Department Clinical Research of Developpement

Principal Investigators

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Jocelyne JUST, MdPh

Role: PRINCIPAL_INVESTIGATOR

Assistance Publique - Hôpitaux de Paris

Locations

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Hôpital Trousseau

Paris, , France

Site Status

Countries

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France

Other Identifiers

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AOR05005

Identifier Type: -

Identifier Source: secondary_id

P070201

Identifier Type: -

Identifier Source: org_study_id

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