The Effect of a Probiotic Intervention in Healthy Subjects and Patients With Atopic Dermatitis

NCT ID: NCT00550472

Last Updated: 2008-04-15

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

30 participants

Study Classification

INTERVENTIONAL

Study Start Date

2004-12-31

Study Completion Date

2005-07-31

Brief Summary

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The study was performed to investigate the effects of a probiotic supplementation containing Lactobacillus paracasei Lpc-37, Lactobacillus acidophilus 74-2 and Bifidobacterium animalis subsp. lactis DGCC 420 (B. lactis 420) on clinical, immunological and faecal parameters in healthy volunteers and in patients with atopic dermatitis (AD).

Detailed Description

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Recent studies suggest that oral bacteriotheraphy with probiotic bacteria might be useful to alleviate atopic dermatitis in infants. There are few indications about the effect of probiotics in the management of atopic dermatitis in adults.

As a precondition for participating in this study, the subjects were provided information in writing and verbally about the details of the study. Informed consent was obtained from all volunteers. Before the beginning of the study, all participants were subject to a medical examination in the Clinic for Dermatology and Dermatologic Allergology of the Friedrich Schiller University Jena. The AD was diagnosed according to the atopy-score of Diepgen. Severity of eczema was evaluated by using the SCORAD score. A skin prick test was performed for the exclusion of type 1 sensitivity to cow's milk.

The study was designed as a placebo-controlled and cross-over study. After a 3-week run-in period the 30 participants (15 healthy subjects and 15 patients with AD) were randomized to receive either 200 ml/d of a probiotic drink containing L. paracasei Lpc-37, L. acidophilus 74-2 and B. lactis 420 or 200 ml/d of a placebo drink for 8 weeks. After a 2-week washout period the intervention was crossed between the groups and the respective products were consumed for another 8 weeks followed by a 2-week washout period. Venous blood and fresh stool samples were collected before the beginning and at the end of each period. In AD patients the Scoring of atopic dermatitis (SCORAD) was assessed by the same physician every 4 weeks and 2 weeks after the wash-out period, respectively.

Conditions

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Atopic Dermatitis

Keywords

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probiotics atopic dermatitis immune system human Lactobacillus Bifidobacterium healthy subjects

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

TREATMENT

Blinding Strategy

TRIPLE

Participants Investigators Outcome Assessors

Study Groups

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Probiotic

intervention

Group Type EXPERIMENTAL

probiotics

Intervention Type DIETARY_SUPPLEMENT

Lactobacillus paracasei Lpc-37, Lactobacillus acidophilus 74-2 Bifidobacterium animalis subsp. lactis DGCC 420

Interventions

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probiotics

Lactobacillus paracasei Lpc-37, Lactobacillus acidophilus 74-2 Bifidobacterium animalis subsp. lactis DGCC 420

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

Healthy subjects:

* No allergic asthma
* No allergic rhinoconjunctivitis
* No food allergies
* Exclusion of atopic diathesis (\< 7 points in Erlangen atopy score)

Patients with AD:

* Clear diagnosis of atopic dermatitis (\> 10 points in the Erlangen atopy score) or flexural eczema or pruritis for at least 12 months (recidivating or permanent)
* SCORAD 5-30 (minor or medium AD)
* Willingness to apply for the duration of the study exclusively the cosmetic products recommended by the accompanying doctor and to use the recommended class II corticosteroid (Advantan) as further therapy of AD (for example during an episode)

Exclusion Criteria

* Pregnancy, lactation
* Immune suppressive or cytostatic therapy or systemic steroids \< 1 month before the first intake
* Phototherapy or systemic therapy of AD \< 1 month before the first intake
* Active infections of the skin
* Relevant asthma disease, which must be treated with inhalative corticoids (FEV1 \< 70 %)
* Patients with an indigestibility of/allergy against the components of milk/lactose intolerance (previously known or detected in the allergy test)
* Patients under long-term treatment with systemic steroids, depot steroids, long-acting antihistamines, tranquilizers, psychopharmaceuticals
* Intake of antihistamines, psychopharmaceuticals with antihistaminic effect and application of steroid containing cream to the forearms within the past 7 days before prick testing resp. treatment of the patient with Astemizol (Hismanal) within 4 weeks before the test. Such patients must be excluded from the study because of the long half-life of the medicament.
* Acute or chronic symptomatic heart disease or severe internistic diseases
* Autoimmune diseases, immune deficiencies (including immune suppressive treatment)
* Immune-complex-induced immunopathies or malignant tumors
* Abuse of alcohol, drugs or medicaments
Minimum Eligible Age

18 Years

Maximum Eligible Age

40 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Jena

OTHER

Sponsor Role lead

Responsible Party

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Dept. of Nutritional Physiology

Principal Investigators

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Gerhard Jahreis, Prof. Dr.

Role: PRINCIPAL_INVESTIGATOR

University of Jena, Dept. of Nutritional Physiology

Locations

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University of Jena, Institute of Nutrition, Department of Nutritional Physiology

Jena, Thuringia, Germany

Site Status

Countries

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Germany

Other Identifiers

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LSEP H18-05

Identifier Type: -

Identifier Source: org_study_id