HLA-Mismatched Peripheral Blood Mobilized Hematopoietic Precursor Cell Transplantation for Hematological Malignancies

NCT ID: NCT00001748

Last Updated: 2008-03-04

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE1
• Total Enrollment: 35 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 1998-06-30
• Study Completion Date: 2000-05-31

Brief Summary

Many patients with hematological malignancies potentially curable by bone marrow transplantation are not considered for transplantation because an HLA identical family or unrelated donor is unavailable. For these patients the only curative option is a transplant from a partially matched family donor. Such transplants are feasible but are less successful than matched sibling donor transplants. The main problems with mismatched transplants are graft rejection, graft-vs-host disease, and regimen-related mortality. This restricts the use of mismatched transplants to patients less than 45 years at high risk of dying from the hematological malignancy.

This protocol evaluates a new preparative regimen designed to ensure stem cell engraftment by increased immunosuppression, followed by a G-CSF mobilized T cell depleted, stem cell rich, peripheral blood progenitor cell (PBPC) transplant from a mismatched related donor in patients with high risk hematological malignancies.

This phase I study evaluates engraftment and GVHD following T cell depleted, HLA-mismatched PBPC transplants. Stopping rules will be used to make modifications to the protocol in the event of graft failure.

The end points of the study are graft take, acute and chronic GVHD, leukemic relapse, transplant-related mortality, death and leukemia-free survival. Patients will be followed up for 5 years. It is planned to treat up to 35 patients aged between 10 and 45 years.

Detailed Description

Many patients with hematological malignancies potentially curable by bone marrow transplantation are not considered for transplantation because an HLA identical family or unrelated donor is unavailable. For these patients the only curative option is a transplant from a partially matched family donor. Such transplants are feasible but are less successful than matched sibling donor transplants. The main problems with mismatched transplants are graft rejection, graft-vs-host disease, and regimen-related mortality. This restricts the use of mismatched transplants to patients less than 45 years at high risk of dying from the hematological malignancy.

This protocol evaluates a new preparative regimen designed to ensure stem cell engraftment by increased immunosuppression, followed by a G-CSF mobilized T cell depleted, stem cell rich, peripheral blood progenitor cell (PBPC) transplant from a mismatched related donor in patients with high risk hematological malignancies.

This phase I study evaluates engraftment and GVHD following T cell depleted, HLA-mismatched PBPC transplants. Stopping rules will be used to make modifications to the protocol in the event of graft failure.

The end points of the study are graft take, acute and chronic GVHD, leukemic relapse, transplant-related mortality, death and leukemia-free survival. Patients will be followed up for 5 years. It is planned to treat up to 35 patients aged between 10 and 45 years.

Conditions

Graft vs Host Disease; Hematologic Neoplasms; Lymphoma; Myelodysplastic Syndromes; Myeloid Leukemia

Study Design

• Primary Study Purpose: TREATMENT

Interventions

Peripheral blood progenitor cell transplant

Intervention Type: PROCEDURE

Eligibility Criteria

Inclusion Criteria

Patient:

Ages 10-45 years.

Chronic myelogenous leukemia, any of these categories: accelerated phase or blast transformation.

Acute lymphoblastic leukemia, any of these categories: Adults (greater than 18 years) in any remission with high-risk features (presenting leukocyte count greater than 100,000/cu mm, Karyotypes t9; 22, t4, t19, t11, biphenotypic leukemia). All second remissions, primary induction failure including partial remission, partially responding or untreated relapse.

Acute myelogenous leukemia (AML): All AML in second or subsequent remission, primary induction failure or partial remission and resistant relapse.

Myelodysplastic syndromes, any of these categories: refractory anemia with excess of blasts, transformation to acute leukemia, chronic myelomonocytic leukemia.

Myeloproliferative disorders undergoing transformation to terminal stages.

Chronic lymphocytic leukemia (CLL) in Richter transformation.

High-grade lymphoma, refractory to standard treatment approaches, mantle cell lymphoma.

No major organ dysfunction precluding transplantation.

DLCO greater than 65% predicted.

Left ventricular ejection fraction: greater than 40% predicted.

ECOG performance status of 0 or 1.

Informed consent given. Informed consent from parents for minors.

Women of childbearing age with a negative pregnancy test may participate.

Donor:

Partially HLA matched family donor (3-5/6 matches).

Fit to receive G-CSF and give peripheral blood stem cells (normal blood count, normotensive, and no history of stroke).

Informed consent given.

Patients or donors must not be pregnant or nursing.

Must not have ECOG performance status of 2 or more.

No severe psychiatric illness in patient or donor: Mental deficiency sufficiently severe as to make compliance with the BMT treatment unlikely and making informed consent impossible.

No major anticipated illness or organ failure incompatible with survival from BMT.

DLCO must not be less than 65% predicted.

No left ventricular ejection fraction: less than 40% predicted.

Must not have serum creatinine greater than 3 mg/dl.

Must not have serum bilirubin greater than 4 mg/dl, Transaminases greater than 3 times the upper limit of normal.

Donor or patient must not be HIV positive.

Must not have history of other malignancies except basal cell or squamous carcinoma of the skin, positive PAP smear and subsequent negative follow up.

Donor must be fit to receive G-CSF and undergo apheresis.

Must not fail to mobilize adequate numbers of CD34+ cells after two cycles of G-CSF.

• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

National Heart, Lung, and Blood Institute (NHLBI)

NIH

Sponsor Role: lead

Locations

National Heart, Lung and Blood Institute (NHLBI)

Bethesda, Maryland, United States

Countries

United States

References

Lane TA, Law P, Maruyama M, Young D, Burgess J, Mullen M, Mealiffe M, Terstappen LW, Hardwick A, Moubayed M, et al. Harvesting and enrichment of hematopoietic progenitor cells mobilized into the peripheral blood of normal donors by granulocyte-macrophage colony-stimulating factor (GM-CSF) or G-CSF: potential role in allogeneic marrow transplantation. Blood. 1995 Jan 1;85(1):275-82.

Reference Type: BACKGROUND

PMID: 7528570 (View on PubMed)

Other Identifiers

98-H-0122

Identifier Type: -

Identifier Source: secondary_id

980122

Identifier Type: -

Identifier Source: org_study_id