Glucagon Resistance in Patients With MASLD and T2DM

NCT ID: NCT07246421

Last Updated: 2026-01-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

24 participants

Study Classification

INTERVENTIONAL

Study Start Date

2026-01-01

Study Completion Date

2028-07-31

Brief Summary

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The goal of this clinical trial is to investigate the sensitivity to glucagon in patients with type 2 diabetes mellitus (T2DM), with and without metabolic associated fatty liver disease (MASLD).

The main questions it aims to answer are:

1. Is the sensitivity to glucagon with respect to hepatic FA oxidation and suppression of VLDL-TG secretion impaired in humans with T2DM and MASLD?
2. Is glucagon resistance and MASLD reflected in an aberrated lipidomic/metabolomic profile in blood and adipose tissue?

Researchers will compare patients with T2DM with and without MASLD to see if the response to basal and high levels of glucagon differs between the groups.

Participants will attend 2 short visits and 1 full-day visit, including:

* Body scan (DXA) to check fat and bone composition
* MRI to measure liver fat.
* Blood tests.
* Ultrasound to check liver stiffness and scarring.
* Fat biopsies
* 8-hour hormone (including glucagon) and tracer infusion
* PET-CT scans

Detailed Description

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Conditions

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Metabolic Associated Fatty Liver Disease Type 2 Diabetes (T2DM)

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Subjects with T2DM and MR spectroscopy verified NO steatosis

Group Type ACTIVE_COMPARATOR

Glugagon

Intervention Type OTHER

Infusion of low dose glucagon and high dose glucagon during simultaneous somatostatin infusion and replacement doses of insulin and growth hormone. Infusion of palmitate, VLDL-triglyceride and glucose tracers.

\[11C\]palmitate PET during low and high dose glucagon.

Subjects with T2DM and MR spectroscopy verified steatosis

Group Type ACTIVE_COMPARATOR

Glugagon

Intervention Type OTHER

Infusion of low dose glucagon and high dose glucagon during simultaneous somatostatin infusion and replacement doses of insulin and growth hormone. Infusion of palmitate, VLDL-triglyceride and glucose tracers.

\[11C\]palmitate PET during low and high dose glucagon.

Interventions

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Glugagon

Infusion of low dose glucagon and high dose glucagon during simultaneous somatostatin infusion and replacement doses of insulin and growth hormone. Infusion of palmitate, VLDL-triglyceride and glucose tracers.

\[11C\]palmitate PET during low and high dose glucagon.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* BMI \> 26 kg/m²
* confirmed diagnosis of Type 2 Diabetes Mellitus (T2DM) min. 6 months prior enrollment
* steatosis FF% \> 5,6% on MR spectroscopy for MAFLD group

Exclusion Criteria

* Alcohol abuse (\>10 units per week for both sexes) or other substance abuse
* Smoking
* Current or previous malignant disease
* Blood donation within the last 3 months prior to the study day
* Participation in studies involving radioactive isotopes within the past 3 months
* Pregnancy
* Severely dysregulated type 2 diabetes mellitus (haemoglobin A1c ≥ 100 mmol/mol)
* C-peptide \< 200 pmol/L
* Previous acute myocardial infarction (AMI)
* Clinical symptoms of heart failure
* Current or previous malignant disease
* Known ongoing systemic disease, except for dyslipidaemia and hypertension
* Regular use of medication that may affect lipid and glucose metabolism, including insulin treatment, regular use of over-the-counter medications, and hormonal contraception. Exceptions:

1. Participants treated with statins may be included following a 2-week washout period prior to the experimental study day.
2. Participants receiving oral glucose-lowering therapy for T2DM and antihypertensive medication may be included provided that medication is withheld on the study day only.
3. Participants receiving weekly injectable glucagon-like peptide-1 receptor agonists (GLP-1 analogues) may be included following a 1-week washout period prior to the study day.
Minimum Eligible Age

30 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Novo Nordisk A/S

INDUSTRY

Sponsor Role collaborator

University of Aarhus

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Aarhus University Hospital

Aarhus, , Denmark

Site Status RECRUITING

Countries

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Denmark

Central Contacts

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Kia E. Fonfara, Medical Doctor

Role: CONTACT

+4524607114

Facility Contacts

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Kia E. Fonfara, Medical Doctor

Role: primary

+4524607114

Other Identifiers

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0092321

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

MASLD_GLUCA2025

Identifier Type: -

Identifier Source: org_study_id

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