Effects of Intraoperative Warming Methods on Hematologic Inflammatory Indices in Laparoscopic Cholecystectomy

NCT ID: NCT07232251

Last Updated: 2025-11-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Clinical Phase

NA

Total Enrollment

128 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-12-01

Study Completion Date

2026-04-02

Brief Summary

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Perioperative hypothermia is a frequent and preventable complication that may cause adverse outcomes such as increased blood loss, impaired coagulation, and delayed recovery. Various active warming techniques are used to maintain normothermia during anesthesia; however, their comparative effects on systemic inflammatory responses remain unclear.

This randomized controlled clinical trial aims to evaluate the effects of different intraoperative warming methods on hematologic inflammatory indices - including neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and systemic immune-inflammation index (SII) - in patients undergoing elective laparoscopic cholecystectomy under general anesthesia.

A total of eligible adult patients will be randomly assigned into four groups according to the intraoperative warming method applied:

Control Group: No active warming applied.

Forced-Air Warming (FAW) Group: Warming blanket system used throughout surgery.

Fluid Warming (FW) Group: Intravenous fluids warmed to maintain normothermia.

Combined Warming (FAW + FW) Group: Both forced-air and fluid warming applied simultaneously.

Core body temperature and perioperative data will be recorded. Venous blood samples will be obtained preoperatively and 24 hours postoperatively to calculate inflammatory indices.

The primary objective is to determine whether active intraoperative warming techniques modulate postoperative inflammatory markers compared to no warming. Secondary outcomes include intraoperative temperature trends, recovery times, and the incidence of hypothermia-related complications.

The results are expected to identify the most effective warming strategy to minimize inflammation and optimize postoperative recovery in laparoscopic procedures.

Detailed Description

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Unintended perioperative hypothermia (core temperature \<36°C) commonly occurs during laparoscopic surgeries due to anesthesia-induced thermoregulatory impairment, pneumoperitoneum, and cold ambient conditions. Even mild decreases in body temperature can impair coagulation, delay drug metabolism, and alter immune function. Maintaining normothermia is therefore critical for improving surgical outcomes.

While both forced-air warming systems and fluid warming devices are routinely used, limited data exist comparing their individual and combined effects on postoperative inflammatory responses. Hematologic inflammatory indices such as NLR, PLR, and SII provide cost-effective and reproducible markers that reflect systemic inflammation and immune balance.

This prospective, randomized, controlled, parallel-group study will be conducted at Atatürk University Faculty of Medicine, Department of Anesthesiology and Reanimation.

Eligible participants are adult patients (aged 18-65 years, ASA I-II) scheduled for elective laparoscopic cholecystectomy under general anesthesia. Patients with infection, chronic inflammatory disease, hematologic disorder, or those converted to open surgery will be excluded.

Participants will be randomly assigned to one of four groups:

Group C (Control): No active warming; standard passive insulation only.

Group FAW (Forced-Air Warming): Forced-air warming blanket applied from induction until the end of surgery.

Group FW (Fluid Warming): Intravenous fluids administered through a warming device.

Group CF (Combined Warming): Both forced-air warming and fluid warming used together.

Standardized anesthesia induction and maintenance protocols will be followed for all patients. Core temperature will be continuously monitored via nasopharyngeal probe. Temperature, hemodynamic parameters, and perioperative variables will be recorded at fixed intervals.

Blood samples will be obtained at two time points - preoperatively and at 24 hours postoperatively - for complete blood count analysis. The inflammatory indices (NLR, PLR, SII) will be calculated and compared among groups.

Primary Outcome:

Postoperative change in hematologic inflammatory indices \[(neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and systemic immune-inflammation index (SII)\].

Secondary Outcomes:

Intraoperative temperature maintenance, recovery characteristics, and hypothermia-related adverse events.

Statistical analyses will be performed using SPSS. Continuous variables will be expressed as mean ± SD or median (IQR) and analyzed using ANOVA or Kruskal-Wallis test, as appropriate. Categorical data will be compared using Chi-square or Fisher's exact test. A p-value \<0.05 will be considered statistically significant.

This study seeks to clarify whether intraoperative thermal management strategies influence the systemic inflammatory response, potentially guiding clinicians toward the most effective warming method to enhance recovery and minimize postoperative complications.

Conditions

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Inflammatory Response Perioperative Hypothermia Laparoscopic Cholecystectomy Surgical Stress Response

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

In this prospective, randomized, controlled trial, patients will be equally assigned to four groups:

Control Group (standard blanket + passive blanket)

IV Warmer Group (standard care + intravenous fluid warmer)

External Warmer Group (standard care + external warmer)

Combined Warmer Group (standard care + intravenous fluid warmer + external warmer)
Primary Study Purpose

SUPPORTIVE_CARE

Blinding Strategy

NONE

Study Groups

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Control Group

Patients receive standard intraoperative warming with 38 °C set active warming blanket. Surgical field outside the blanket is covered with passive drapes. No additional warming interventions are applied. (standart care)

Group Type OTHER

Standard Active Warming Blanket

Intervention Type DEVICE

An active blanket set at 38°C is used to maintain the patient's body temperature throughout the operation. Body areas outside the surgical field are covered with passive drapes. No supplemental heating is used.

Intravenous Warmer Group

All standard procedures performed in the control group are followed. Additionally, all intravenous fluids administered during the operation are warmed to 38°C with a fluid warmer.

Group Type ACTIVE_COMPARATOR

Standard Active Warming Blanket

Intervention Type DEVICE

An active blanket set at 38°C is used to maintain the patient's body temperature throughout the operation. Body areas outside the surgical field are covered with passive drapes. No supplemental heating is used.

Intravenous Warmer Group

Intervention Type DEVICE

All intravenous fluids administered during the operation are administered via a fluid warming device at 38°C. All standard practices continue in the control group.

External Warmer Group

All standard approaches used in the control group are performed. Additionally, a special drape is placed over the upper extremities and thorax, which is connected to a 3M Bair Hugger 700 Series device and warmed to 38°C.

Group Type ACTIVE_COMPARATOR

Standard Active Warming Blanket

Intervention Type DEVICE

An active blanket set at 38°C is used to maintain the patient's body temperature throughout the operation. Body areas outside the surgical field are covered with passive drapes. No supplemental heating is used.

External Warmer Group

Intervention Type DEVICE

A special drape is placed over the upper extremities and thorax, and the patient is connected to an external warm air device at 38°C throughout the operation. All standard practices continue in the control group.

Combined Warming Group (Intravenous + External)

All standard approaches used in the control group are performed. Additionally, intravenous fluids are warmed and an external heating device is applied to the upper extremity/thorax.

Group Type ACTIVE_COMPARATOR

Standard Active Warming Blanket

Intervention Type DEVICE

An active blanket set at 38°C is used to maintain the patient's body temperature throughout the operation. Body areas outside the surgical field are covered with passive drapes. No supplemental heating is used.

Intravenous Warmer Group

Intervention Type DEVICE

All intravenous fluids administered during the operation are administered via a fluid warming device at 38°C. All standard practices continue in the control group.

External Warmer Group

Intervention Type DEVICE

A special drape is placed over the upper extremities and thorax, and the patient is connected to an external warm air device at 38°C throughout the operation. All standard practices continue in the control group.

Interventions

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Standard Active Warming Blanket

An active blanket set at 38°C is used to maintain the patient's body temperature throughout the operation. Body areas outside the surgical field are covered with passive drapes. No supplemental heating is used.

Intervention Type DEVICE

Intravenous Warmer Group

All intravenous fluids administered during the operation are administered via a fluid warming device at 38°C. All standard practices continue in the control group.

Intervention Type DEVICE

External Warmer Group

A special drape is placed over the upper extremities and thorax, and the patient is connected to an external warm air device at 38°C throughout the operation. All standard practices continue in the control group.

Intervention Type DEVICE

Other Intervention Names

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MEDWARM W-300/A 150-MS/A 96116-K4656 IV Fluid Warmer Portable Flotherm QW3 Fluid And Blood Warmer External Forced-Air Warming Device 3M Bair Hugger 700 Series

Eligibility Criteria

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Inclusion Criteria

* Patients scheduled for elective laparoscopic cholecystectomy.
* ASA (American Society of Anesthesiologists) physical status I-III.
* Age between 18 and 65 years.
* Willingness and ability to provide written informed consent.

Exclusion Criteria

* ASA IV-V patients.
* Emergency surgery requirement.
* Preoperative fever (\>38.0 °C) or hypothermia (\<36.0 °C).
* Operation duration \<60 minutes or \>180 minutes.
* Endocrine/metabolic disorders affecting body temperature (e.g., moderate-severe thyroid dysfunction, pheochromocytoma, severe dysautonomia, malnutrition).
* Active infection/sepsis or systemic infection within past 2 weeks.
* Chronic immunosuppressive therapy (e.g., ≥10 mg/day prednisolone equivalent ≥2 weeks or biological agents in the past month).
* Hematologic disorders (e.g., leukemia, aplastic anemia, myeloproliferative disorders) or abnormal blood counts (platelet \<100 ×10⁹/L, leukocyte \<3 ×10⁹/L).
* Active malignancy or ongoing chemotherapy/radiotherapy in last 3 months.
* Severe organ failure (Child-Pugh C liver, eGFR \<30 mL/min/1.73 m² or dialysis, NYHA III-IV heart failure, GOLD III-IV COPD).
* Coagulopathy or uncontrolled antithrombotic therapy.
* Pregnancy or lactation.
* Conversion from laparoscopic to open surgery.
* Non-adherence to assigned warming protocol.
* Inability to place esophageal temperature probe or unreliable temperature monitoring.
* Intraoperative hemodynamic instability requiring prolonged vasopressor support or blood transfusion.
* Missing or incomplete preoperative or postoperative CBC preventing calculation of inflammatory indices.
* Withdrawal of consent or loss to follow-up.
Minimum Eligible Age

18 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Ataturk University

OTHER

Sponsor Role lead

Responsible Party

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Erkan Cem ÇELİK

Principal Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Erkan Cem Çelik, doctor

Role: PRINCIPAL_INVESTIGATOR

Ataturk University Department of Anesthesiology and Reanimation

Locations

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Ataturk University

Erzurum, , Turkey (Türkiye)

Site Status

Countries

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Turkey (Türkiye)

Central Contacts

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Erkan Cem Çelik, Doctor

Role: CONTACT

+90-505-344-21-81

Mehmet Akif Yılmaz, assistant doctor

Role: CONTACT

+90-534-653-35-39

Facility Contacts

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Erkan Cem Çelik, doctor

Role: primary

+90-505-344-21-81

Mehmet Akif yılmaz, assistant doctor

Role: backup

+90-534-653-35-39

References

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Wang J, Bi Y, Ma J, He Y, Liu B. Association of Preoperative Neutrophil-to-Lymphocyte Ratio with Postoperative Acute Kidney Injury and Mortality Following Major Noncardiac Surgeries. World J Surg. 2023 Apr;47(4):948-961. doi: 10.1007/s00268-022-06878-2. Epub 2023 Jan 21.

Reference Type RESULT
PMID: 36681771 (View on PubMed)

Jiao Y, Zhang X, Liu M, Sun Y, Ma Z, Gu X, Gu W, Zhu W. Systemic immune-inflammation index within the first postoperative hour as a predictor of severe postoperative complications in upper abdominal surgery: a retrospective single-center study. BMC Gastroenterol. 2022 Aug 27;22(1):403. doi: 10.1186/s12876-022-02482-9.

Reference Type RESULT
PMID: 36030214 (View on PubMed)

Gao Y, Guo W, Cai S, Zhang F, Shao F, Zhang G, Liu T, Tan F, Li N, Xue Q, Gao S, He J. Systemic immune-inflammation index (SII) is useful to predict survival outcomes in patients with surgically resected esophageal squamous cell carcinoma. J Cancer. 2019 Jun 2;10(14):3188-3196. doi: 10.7150/jca.30281. eCollection 2019.

Reference Type RESULT
PMID: 31289589 (View on PubMed)

Xu H, Wang Z, Guan X, Lu Y, Malone DC, Salmon JW, Ma A, Tang W. Safety of intraoperative hypothermia for patients: meta-analyses of randomized controlled trials and observational studies. BMC Anesthesiol. 2020 Aug 15;20(1):202. doi: 10.1186/s12871-020-01065-z.

Reference Type RESULT
PMID: 32799802 (View on PubMed)

Zheng W, Huang B, Bao L, Wang J, Jin J. Comparing warming strategies to reduce hypothermia and shivering in elderly abdominal or pelvic surgery patients: a network meta-analysis. Sci Rep. 2025 Jul 1;15(1):22356. doi: 10.1038/s41598-025-04644-7.

Reference Type RESULT
PMID: 40595941 (View on PubMed)

Ji N, Wang J, Li X, Shang Y. Strategies for perioperative hypothermia management: advances in warming techniques and clinical implications: a narrative review. BMC Surg. 2024 Dec 30;24(1):425. doi: 10.1186/s12893-024-02729-0.

Reference Type RESULT
PMID: 39736577 (View on PubMed)

McSwain J. Perioperative hypothermia: Causes, consequences and treatment. World Journal of Anesthesiology. 11/27 2015;4:58. doi:10.5313/wja.v4.i3.58

Reference Type RESULT

Rauch S, Miller C, Brauer A, Wallner B, Bock M, Paal P. Perioperative Hypothermia-A Narrative Review. Int J Environ Res Public Health. 2021 Aug 19;18(16):8749. doi: 10.3390/ijerph18168749.

Reference Type RESULT
PMID: 34444504 (View on PubMed)

Reynolds L, Beckmann J, Kurz A. Perioperative complications of hypothermia. Best Pract Res Clin Anaesthesiol. 2008 Dec;22(4):645-57. doi: 10.1016/j.bpa.2008.07.005.

Reference Type RESULT
PMID: 19137808 (View on PubMed)

Ruetzler K, Kurz A. Consequences of perioperative hypothermia. Handb Clin Neurol. 2018;157:687-697. doi: 10.1016/B978-0-444-64074-1.00041-0.

Reference Type RESULT
PMID: 30459033 (View on PubMed)

Other Identifiers

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2025/2

Identifier Type: OTHER

Identifier Source: secondary_id

B.30.2.ATA.0.01.00/657

Identifier Type: -

Identifier Source: org_study_id

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