Role of Red Blood Cells in Cardiovascular Disease

NCT ID: NCT07151222

Last Updated: 2025-09-03

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

500 participants

Study Classification

OBSERVATIONAL

Study Start Date

2017-01-01

Study Completion Date

2031-12-30

Brief Summary

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The risk of myocardial infarction is dependent on cardiovascular risk factors including type 2 diabetes (T2D) but underlying mechanisms are poorly understood. We identified that red blood cells (RBCs) mediate beneficial cardiovascular regulatory effects under hypoxic/ischemic conditions via signaling by nitric oxide (NO) and soluble guanylate cyclase (sGC) in the RBCs. The RBCs become dysregulated in T2D which induces endothelial and cardiac injury. This project investigates the signaling of RBCs in cardiovascular disease and explores novel therapeutic strategies that target RBC function in myocardial infarction and T2D.

Aims To determine the

* mechanisms behind cardioprotective effect of RBCs in myocardial infarction
* signaling behind cardiovascular injury induced by RBCs in T2D

Work plan RBCs collected from patients with myocardial infarction, patients with T2D and healthy controls are investigated in bioassays including isolated hearts of ischemia/reperfusion, endothelial function and cell cultures. Molecular mechanisms behind the effects of RBCs are identified with focus on the NO-sGC pathway in the RBCs.

This project unravels the RBC as a mediator of cardiovascular disease and has the potential to identify novel therapeutic strategies by targeting RBC signaling.

Detailed Description

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The function of RBCs are investigated in functional bioassays and cell culture systems. The bioassays include isolated hearts subjected to ischemia-reperfusion and isolated arteries. the readouts are left ventricular function, infarct size and endothelial function . Comparisons are made between RBCs collected from patients with cardiovascular disease, type 2 diabetes and healthy controls. Pharmacological interventions are performed by ex vivo incubations with focus on the NO-sGC pathway.

Conditions

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Diabetes Mellitus

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Type 2 diabetes

Patients with type 2 diabetes

No interventions assigned to this group

Type 1 diabetes

Patients with type 1 diabetes

No interventions assigned to this group

Coronary artery disease/Myocardial infarction

Patients with acute coronary syndromes

No interventions assigned to this group

Healthy controls

Age- and gender-matched healthy subjects

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

* Type 2 diabetes

Exclusion Criteria

\-
Minimum Eligible Age

25 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Karolinska University Hospital

OTHER

Sponsor Role collaborator

Karolinska Institutet

OTHER

Sponsor Role lead

Responsible Party

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John Pernow

Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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John Pernow, MD

Role: PRINCIPAL_INVESTIGATOR

Karolinska Institutet

Locations

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Karolinska Institutet

Stockholm, Swsden, Sweden

Site Status RECRUITING

Countries

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Sweden

Central Contacts

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John Pernow, MD

Role: CONTACT

+46704848361

Facility Contacts

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Stina Smetana, Nurse

Role: primary

+46 725841182

References

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Pernow J, Yang J. Red blood cells: a new target to prevent cardiovascular disease? Eur Heart J. 2024 Oct 21;45(40):4249-4251. doi: 10.1093/eurheartj/ehae454. No abstract available.

Reference Type RESULT
PMID: 39258963 (View on PubMed)

Other Identifiers

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2024-02534

Identifier Type: -

Identifier Source: org_study_id

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