Nicotinamide Mononucleotide Supplementation for Exercise Tolerance Improvement in Healthy Older Adults

NCT ID: NCT07144527

Last Updated: 2025-09-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

Get a concise snapshot of the trial, including recruitment status, study phase, enrollment targets, and key timeline milestones.

Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

40 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-09-15

Study Completion Date

2026-11-01

Brief Summary

Review the sponsor-provided synopsis that highlights what the study is about and why it is being conducted.

This is a randomized, double-blind, placebo-controlled, two-arm crossover clinical trial evaluating the effects of a patented age-reversal therapy, EGA®, compared to conventional nicotinamide mononucleotide (NMN) supplementation. The study will assess safety and efficacy in improving exercise tolerance and modulating biomarkers of aging in healthy older adults.

The primary outcome is cycle ergometry constant work rate (CWR) exercise tolerance, measured as time to fatigue. Secondary outcomes include changes in peak oxygen consumption (VO₂peak), critical power, anaerobic work capacity, lactate threshold, and NAD⁺ metabolite levels.

EGA® is composed of three metabolomic compounds that are endogenous to humans. The formulation has been used in prior exploratory studies and real-world applications, which have helped inform the design of this current trial.

Detailed Description

Dive into the extended narrative that explains the scientific background, objectives, and procedures in greater depth.

This randomized, double-blind, placebo-controlled, two-arm crossover trial will compare the effects of two NAD⁺-modulating interventions-EGA® and conventional nicotinamide mononucleotide (NMN) supplementation-on exercise tolerance and metabolic biomarkers in healthy older adults. EGA® is a proprietary formulation composed of three endogenously occurring metabolomic compounds (including NMN) and has been used in prior non-FDA-regulated studies involving human participants. The EGA® arm will involve a twice-daily oral dose of EGA® containing 1000 mg of NMN, while the comparison arm will involve twice-daily oral dose of 1000 mg of conventional NMN per day. Doses will be self-administered each morning and evening with water.

The primary endpoint is time to fatigue during constant work rate cycle ergometry (CWR-Tlim), a validated surrogate for submaximal exercise tolerance. Secondary endpoints include peak oxygen uptake (VO₂peak), lactate threshold, anaerobic work capacity, and critical power, in addition to circulating NAD⁺ and its related metabolites.

The trial is designed to evaluate the short-term physiological effects and biological activity of EGA® compared to conventional NMN under tightly controlled exercise testing and training conditions. Data from this study will be used to inform the therapeutic potential of EGA® in improving physical performance in healthy older adults (60-80 years). Future directions may include further clinical evaluation or regulatory submissions, depending on outcomes from this pilot investigation.

Conditions

See the medical conditions and disease areas that this research is targeting or investigating.

Aging Frailty Exercise Tolerance Healthy Male and Female Subjects

Study Design

Understand how the trial is structured, including allocation methods, masking strategies, primary purpose, and other design elements.

Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Randomized, double-blind trial with two parallel groups, each using a two-period crossover design. One group receives EGA and a placebo in a random sequence, and the other group receives NMN and a placebo in a random sequence.
Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

Review each arm or cohort in the study, along with the interventions and objectives associated with them.

Arm 1 - EGA -> Placebo

Participants receive EGA during Period 1, followed by Placebo during Period 2 after a washout. Assigned Interventions: EGA; Placebo.

Group Type EXPERIMENTAL

NMN, betaine, low-dose hydrogen peroxide (EGA)

Intervention Type DIETARY_SUPPLEMENT

Proprietary formulation containing NMN, betaine, and low-dose hydrogen peroxide.

Placebo

Intervention Type DIETARY_SUPPLEMENT

Organoleptically matched inert control.

Arm 2 - Placebo -> EGA

Participants receive Placebo during Period 1, followed by EGA during Period 2 after a washout. Assigned Interventions: Placebo; EGA.

Group Type EXPERIMENTAL

NMN, betaine, low-dose hydrogen peroxide (EGA)

Intervention Type DIETARY_SUPPLEMENT

Proprietary formulation containing NMN, betaine, and low-dose hydrogen peroxide.

Placebo

Intervention Type DIETARY_SUPPLEMENT

Organoleptically matched inert control.

Arm 3 - NMN -> Placebo

Participants receive Nicotinamide Mononucleotide (NMN) during Period 1, followed by Placebo during Period 2 after a washout. Assigned Interventions: NMN; Placebo.

Group Type EXPERIMENTAL

Nicotinamide Mononucleotide (NMN)

Intervention Type DIETARY_SUPPLEMENT

Conventional NMN supplement.

Placebo

Intervention Type DIETARY_SUPPLEMENT

Organoleptically matched inert control.

Arm 4 - Placebo -> NMN

Participants receive Placebo during Period 1, followed by NMN during Period 2 after a washout. Assigned Interventions: Placebo; NMN.

Group Type EXPERIMENTAL

Nicotinamide Mononucleotide (NMN)

Intervention Type DIETARY_SUPPLEMENT

Conventional NMN supplement.

Placebo

Intervention Type DIETARY_SUPPLEMENT

Organoleptically matched inert control.

Interventions

Learn about the drugs, procedures, or behavioral strategies being tested and how they are applied within this trial.

NMN, betaine, low-dose hydrogen peroxide (EGA)

Proprietary formulation containing NMN, betaine, and low-dose hydrogen peroxide.

Intervention Type DIETARY_SUPPLEMENT

Nicotinamide Mononucleotide (NMN)

Conventional NMN supplement.

Intervention Type DIETARY_SUPPLEMENT

Placebo

Organoleptically matched inert control.

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

Check the participation requirements, including inclusion and exclusion rules, age limits, and whether healthy volunteers are accepted.

Inclusion Criteria

* Adults aged 60 to 80 years, of any sex
* Written clearance from primary care physician confirming medical fitness to participate in an exercise-based clinical study
* Normal or near-normal cardiovascular function to safely engage in moderate to vigorous exercise, demonstrated by either: stress echocardiogram or coronary CT angiogram within the past 12 months showing normal left ventricular function, no significant valvular heart disease, no ischemic changes, and no other clinically significant abnormalities, plus confirmation that no new cardiovascular symptoms (e.g., chest pain, dyspnea, syncope) or changes in health status have occurred since testing
* Montreal Cognitive Assessment (MoCA) score of 26 or higher
* Free from acute or uncontrolled chronic medical conditions that would pose a risk or interfere with study participation
* Physically capable of engaging in structured exercise involving moderate to vigorous intensity stationary cycling
* Successful completion of a graded exercise test (e.g., stationary bike) without significant adverse events or contraindications
* Ability to attend at least four in-person laboratory visits in Beverly Hills, California
* Non-smoker for at least 12 months
* Able to provide informed consent and comply with study procedures
* Willing to refrain from initiating new exercise, dietary supplement, or medication regimens during the study

Exclusion Criteria

* Severe cardiovascular disease (e.g., recent myocardial infarction \<6 months, unstable angina, uncontrolled hypertension \>160/100 mmHg)
* Severe respiratory conditions (e.g., advanced COPD or other conditions preventing exercise participation)
* Significant neurological impairments that hinder comprehension of instructions or participation in exercise
* Terminal illness or conditions limiting life expectancy or ability to complete the study
* Severe cognitive impairment preventing informed consent, protocol adherence, or comprehension of interventions
* Significant orthopedic limitations or injuries that prevent safe exercise participation
* Use of NMN, NR, or other NAD+-altering supplements within the past 14 weeks
* Anticipated or current alcohol consumption \>2 drinks per week, or unwillingness to abstain from alcohol during the study
* Current substance abuse affecting study participation or adherence
* Other acute or chronic health conditions judged by the investigators to pose risk or interfere with study objectives
* Non-compliance with study requirements, withdrawal of informed consent, or unforeseen circumstances compromising completion
* Any condition which, in the opinion of the investigator, would interfere with study participation or interpretation of results
Minimum Eligible Age

60 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

Meet the organizations funding or collaborating on the study and learn about their roles.

Concordia University Chicago

OTHER

Sponsor Role collaborator

EGACeutical

UNKNOWN

Sponsor Role collaborator

Scott Silveira

OTHER

Sponsor Role lead

Responsible Party

Identify the individual or organization who holds primary responsibility for the study information submitted to regulators.

Scott Silveira

Principle Investigator

Responsibility Role SPONSOR_INVESTIGATOR

Locations

Explore where the study is taking place and check the recruitment status at each participating site.

Medical Office of Dr. Robert Huizenga MD

Beverly Hills, California, United States

Site Status RECRUITING

Countries

Review the countries where the study has at least one active or historical site.

United States

Facility Contacts

Find local site contact details for specific facilities participating in the trial.

Nancy Castellanos, BA

Role: primary

310-666-6585

Ashley Erazo, Medical Assistant

Role: backup

310-657-9191

Other Identifiers

Review additional registry numbers or institutional identifiers associated with this trial.

2305604-1

Identifier Type: -

Identifier Source: org_study_id

More Related Trials

Additional clinical trials that may be relevant based on similarity analysis.

NR Supplementation and Exercise
NCT04907110 COMPLETED NA
NEUROmuscular Training for Enhanced AGE Longevity
NCT06620666 ACTIVE_NOT_RECRUITING NA