Oxidative DNA Damage Enhancement by Vitamin C Among Narghile Smokers and Non-smokers

NCT ID: NCT07141823

Last Updated: 2025-08-26

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

96 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-10-15

Study Completion Date

2025-03-10

Brief Summary

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This study aims to evaluate oxidative DNA damage among hookah smokers compared to non-smokers by measuring biomarkers of oxidative stress in blood samples. The objective is to determine the impact of hookah smoking on DNA integrity and overall oxidative status in adults.

Detailed Description

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Hookah smoking has become increasingly popular in many regions, yet its potential impact on oxidative stress and DNA integrity is not fully understood. This study aims to investigate the level of oxidative DNA damage among hookah smokers compared to non-smokers by analyzing biomarkers in blood samples. A case-control observational design will be used, involving two groups of adult participants: regular hookah smokers and age-matched non-smokers. Blood samples will be collected from all participants and analyzed for markers of oxidative stress, including 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA), using validated biochemical methods. The findings are expected to provide insights into the biological consequences of hookah smoking and support the development of public health interventions.

Conditions

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Narghile

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

SINGLE

Participants

Study Groups

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Narghile nonsmokers vitamin C taking

this group of participant take vitamin C for one month, and they are healthy volunteers

Group Type EXPERIMENTAL

Vitamin C (Ascorbic Acid) regularly has been taken

Intervention Type DIETARY_SUPPLEMENT

Narghile smokers

this group take vitamin c supplent for one month period and are healthy.

Group Type EXPERIMENTAL

Vitamin C (Ascorbic Acid) regularly has been taken

Intervention Type DIETARY_SUPPLEMENT

Interventions

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Vitamin C (Ascorbic Acid) regularly has been taken

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* Adults aged 18-30 years

Current narghile (hookah) smokers for at least 1 year

Willing to provide informed consent

Exclusion Criteria

* Healthy participant
Minimum Eligible Age

18 Months

Maximum Eligible Age

30 Months

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Nora Tariq Mohammed

OTHER

Sponsor Role lead

Responsible Party

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Nora Tariq Mohammed

MSc Student in Biochemistry

Responsibility Role SPONSOR_INVESTIGATOR

Locations

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University of Duhok

Dihok, semel, Iraq

Site Status

Countries

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Iraq

Other Identifiers

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UoD-ODNS-2025-01

Identifier Type: -

Identifier Source: org_study_id

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