Validation of Donor-Derived Cell-Free DNA (Dd-cfDNA) for Kidney Transplant Monitoring

NCT ID: NCT07060716

Last Updated: 2025-11-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

125 participants

Study Classification

OBSERVATIONAL

Study Start Date

2025-09-08

Study Completion Date

2025-12-31

Brief Summary

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The goal of this observational study is to learn if the donor-derived cell-free DNA (dd-cfDNA) test can assess rejection in kidney transplant recipients. Participants will have blood and urine collected at their study visit.

Researchers will compare results of the GraftAssureDx to rejection detected by standard-of-care graft biopsies.

Detailed Description

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Conditions

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Kidney Transplant Rejection Cell-free DNA

Study Design

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Observational Model Type

CASE_ONLY

Study Time Perspective

PROSPECTIVE

Interventions

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donor-derived cell-free DNA test

A donor-derived cell-free DNA (dd-cfDNA) test used to measure the concentration of total cell-free DNA and the fractional abundance of the dd-cfDNA.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

1. Subject is 18 years of age or older
2. At least 12 calendar days have elapsed since the subject received a kidney transplant.
3. Subject has provided legally effective informed consent
4. Subject agrees to comply with all study procedures


1\. A graft biopsy is obtained within ±1 week of blood draw.

* If blood draw is obtained after biopsy, blood draw should be taken at least 2 days after an uncomplicated biopsy procedure.

Exclusion Criteria

1. Kidney donor is an identical twin of the subject.
2. The subject has another previously transplanted organ in situ.
3. Subject has received a hematopoietic stem cell transplant.
4. Subject has received a bone marrow graft.
5. Subject has self-reported as pregnant.
6. In the opinion of the investigator, the subject's participation in the study would pose a risk to data integrity or to the subject's safety and welfare.


1. Sample collected from someone that had an invasive graft biopsy ≤ 48 hours prior to blood draw.
2. Sample collected from subject that received immunosuppressive treatment for biopsy-proven acute rejection ≤ 30 days prior to blood draw
3. Sample collected from subject that received a blood transfusion ≤ 30 days prior to blood draw.
4. Sample collected from a subject that provided another sample for the study within the past 7 days.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Insight Molecular Diagnostics

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Ekkehard Schuetz, MD, PhD

Role: STUDY_CHAIR

Insight Molecular Diagnostics

Locations

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University of Southern California Keck School of Medicine

Los Angeles, California, United States

Site Status ACTIVE_NOT_RECRUITING

Mayo Clinic in Florida

Jacksonville, Florida, United States

Site Status RECRUITING

Tampa General Hospital

Tampa, Florida, United States

Site Status RECRUITING

Cleveland Clinic

Cleveland, Ohio, United States

Site Status RECRUITING

University of Pittsburgh Medical Center

Pittsburgh, Pennsylvania, United States

Site Status ACTIVE_NOT_RECRUITING

Vanderbilt University Medical Center

Nashville, Tennessee, United States

Site Status RECRUITING

Baylor, Scott & White Research Institute

Dallas, Texas, United States

Site Status RECRUITING

Intermountain Health

Murray, Utah, United States

Site Status RECRUITING

Institute of Immunology - Transplantation Immunology

Heidelberg, Baden-Wurttemberg, Germany

Site Status ACTIVE_NOT_RECRUITING

Charite Universitatsmedizin

Berlin, , Germany

Site Status ACTIVE_NOT_RECRUITING

Countries

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United States Germany

Central Contacts

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Robert Rogers, M.S.

Role: CONTACT

14436258427

Ekkehard Schuetz, MD, PhD

Role: CONTACT

615-927-1528

Facility Contacts

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Carla Palmucci

Role: primary

904-953-3182

Anelys Marin, B.S.

Role: primary

813-844-2673

Sandra Sanchez

Role: backup

Jennifer Seaman-Czerr

Role: primary

216-990-0436

Keyann Reaves

Role: primary

615-936-0695

Eric Javier Martinez, MD

Role: primary

214-820-2050

Ashley McAllister

Role: backup

214-820-1710

Shannon Sell

Role: primary

801-507-8390

Other Identifiers

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CLN-001

Identifier Type: -

Identifier Source: org_study_id

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