Smart Measurement of Circulating Tumor DNA

NCT ID: NCT06989814

Last Updated: 2025-08-11

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

50 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-05-16

Study Completion Date

2027-02-01

Brief Summary

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The goal of this study is to develop a blood-test which can detect colorectal cancer in early stages.

Participants will be asked to take an extra blood test, which will be analyzed further in the lab.

Detailed Description

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Lynch Syndrome (LS) carriers have a predisposition to develop various types of cancer, especially colorectal cancer (CRC) and endometrial cancer (EC). LS patients are advised to undergo surveillance by colonoscopy every 2 year and gynaecological surveillance. This surveillance is deemed burdensome and fails to detect a small part of the developing CRCs and the majority of extra-colonic cancers. To ensure prevention and early detection of cancer, a reliable and accessible test is needed. Recent studies have shown the potential of the detection of tumor-derived DNA fragments (circulating tumor DNA; ctDNA). Various molecular characteristics can be used to discriminate ctDNA from healthy circulating cell-free DNA. Current ctDNA assays with the highest sensitivity and specificity to detect for example minimal residual disease (MRD) after surgery are mostly tumor-informed, which means prior information is needed from the tumor tissue about the molecular alterations present.

As this information is not available for the detection of newly arising tumors, the aim of this study is to evaluate the use of an optimized combination of tumor agnostic ctDNA characteristics for the detection of newly developing tumors.

Conditions

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Colorectal Neoplasms Colorectal Neoplasms Malignant Colorectal Neoplasms, Hereditary Nonpolyposis Hereditary Nonpolyposis Colon Cancer

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Case-control study.
Primary Study Purpose

SCREENING

Blinding Strategy

NONE

Study Groups

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Lynch-carriers with a (colorectal) tumor

People with a newly diagnosed Lynch-associated tumor will be asked to undergo an extra blood test.

Group Type EXPERIMENTAL

Blood Product

Intervention Type DIAGNOSTIC_TEST

An (extra) blood test will be done on participants. This will be analyzed for circulating tumor DNA (ctDNA) via a multi-omics approach.

Interventions

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Blood Product

An (extra) blood test will be done on participants. This will be analyzed for circulating tumor DNA (ctDNA) via a multi-omics approach.

Intervention Type DIAGNOSTIC_TEST

Other Intervention Names

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Blood test Blood draw Liquid biopsy

Eligibility Criteria

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Inclusion Criteria

(suspect) Lynch Syndrome carriers who:

* Have proven Lynch Syndrome (MMR-gene or EPCAM mutation), or have a proven microsatellite instability high (MSI-H)tumor;
* Are at least 18 years old; Have been diagnosed with any form of cancer at the time of inclusion, but have had no treatment yet;
* Have granted informed consent to participate in this study.

Exclusion Criteria

(suspect) Lynch Syndrome carriers who:

* Are unwilling to undergo extra blood sampling;
* Are under the age of 18;
* Have no newly diagnosed tumors at time of inclusion;
* Have been treated for their tumor at time of inclusion;
* Are not able to read or understand Dutch language or are mentally not capable.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Dutch Cancer Society

OTHER

Sponsor Role collaborator

Leiden University Medical Center

OTHER

Sponsor Role collaborator

Erasmus Medical Center

OTHER

Sponsor Role lead

Responsible Party

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Lotte van Leeuwen

PhD Candidate

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Anja Wagner, Dr.

Role: PRINCIPAL_INVESTIGATOR

Erasmus Medical Center

Locations

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Leiden University Medical Center

Leiden, South Holland, Netherlands

Site Status NOT_YET_RECRUITING

Erasmus MC

Rotterdam, South Holland, Netherlands

Site Status RECRUITING

Countries

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Netherlands

Central Contacts

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Lotte van Leeuwen, BSc

Role: CONTACT

+31646245198

Facility Contacts

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Maartje Nielsen, Dr.

Role: primary

+31624240843

Lotte van Leeuwen, BSc

Role: primary

0646245198

References

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Mouliere F, Chandrananda D, Piskorz AM, Moore EK, Morris J, Ahlborn LB, Mair R, Goranova T, Marass F, Heider K, Wan JCM, Supernat A, Hudecova I, Gounaris I, Ros S, Jimenez-Linan M, Garcia-Corbacho J, Patel K, Ostrup O, Murphy S, Eldridge MD, Gale D, Stewart GD, Burge J, Cooper WN, van der Heijden MS, Massie CE, Watts C, Corrie P, Pacey S, Brindle KM, Baird RD, Mau-Sorensen M, Parkinson CA, Smith CG, Brenton JD, Rosenfeld N. Enhanced detection of circulating tumor DNA by fragment size analysis. Sci Transl Med. 2018 Nov 7;10(466):eaat4921. doi: 10.1126/scitranslmed.aat4921.

Reference Type BACKGROUND
PMID: 30404863 (View on PubMed)

Deger T, Boers RG, de Weerd V, Angus L, van der Put MMJ, Boers JB, Azmani Z, van IJcken WFJ, Grunhagen DJ, van Dessel LF, Lolkema MPJK, Verhoef C, Sleijfer S, Martens JWM, Gribnau J, Wilting SM. High-throughput and affordable genome-wide methylation profiling of circulating cell-free DNA by methylated DNA sequencing (MeD-seq) of LpnPI digested fragments. Clin Epigenetics. 2021 Oct 20;13(1):196. doi: 10.1186/s13148-021-01177-4.

Reference Type BACKGROUND
PMID: 34670587 (View on PubMed)

Schrag D, Beer TM, McDonnell CH 3rd, Nadauld L, Dilaveri CA, Reid R, Marinac CR, Chung KC, Lopatin M, Fung ET, Klein EA. Blood-based tests for multicancer early detection (PATHFINDER): a prospective cohort study. Lancet. 2023 Oct 7;402(10409):1251-1260. doi: 10.1016/S0140-6736(23)01700-2.

Reference Type BACKGROUND
PMID: 37805216 (View on PubMed)

Eikenboom EL, Wilting SM, Deger T, Srebniak MI, Van Veghel-Plandsoen M, Boers RG, Boers JB, van IJcken WFJ, Gribnau JH, Atmodimedjo P, Dubbink HJ, Martens JWM, Spaander MCW, Wagner A. Liquid Biopsies for Colorectal Cancer and Advanced Adenoma Screening and Surveillance: What to Measure? Cancers (Basel). 2023 Sep 17;15(18):4607. doi: 10.3390/cancers15184607.

Reference Type RESULT
PMID: 37760576 (View on PubMed)

Provided Documents

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Document Type: Study Protocol and Statistical Analysis Plan

View Document

Related Links

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Other Identifiers

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14976

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

NL87476.078.24

Identifier Type: -

Identifier Source: org_study_id

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