Comparison and Strategy Optimization of Plasma EBV DNA and P85-Ab with VCA/EBNA1-IgA for Screening Nasopharyngeal Carcinoma in High-risk Areas

NCT ID: NCT06870435

Last Updated: 2025-03-11

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

68649 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-01-24

Study Completion Date

2035-12-31

Brief Summary

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This study is a prospective, self-controlled, multicenter clinical trial. All participants will be tested for Epstein-Barr virus (EBV) associated biomarkers, including the two-antibody method (VCA-IgA and EBNA1-IgA), BNLF2b total antibodies (P85-Ab), and plasma EBV DNA. Furthermore, novel screening biomarkers, such as next-generation sequencing for EBV and castoff cells using nasopharyngeal swabs, will be explored.

First, it aims to investigate whether plasma EBV DNA testing or the P85-Ab testing can achieve higher sensitivity than the current standard two-antibody method testing while maintaining specificity in NPC screening, thereby identifying the optimal initial NPC screening strategy. Based on the determined optimal initial screening strategy, the study will validate the proposed two-step method (subjects first undergo two-antibody method testing and P85-Ab testing; those positive for either one biomarker above proceed to plasma EBV DNA testing; subjects positive in both steps are defined as high-risk and receive endoscopic examinations with or without biopsy) compared with the single-step method (subjects simultaneously undergo two-antibody method testing, P85-Ab testing, and plasma EBV DNA testing; subjects with any positive biomarker undergo endoscopic examinations with or without biopsy) and each single screening testing. The aim is to determine whether two-step method can further improve the positive predictive value (PPV) while maintaining non-inferior sensitivity, thereby enhancing screening efficiency, reducing the rate of invasive procedures (such as endoscopic biopsies), and lowering medical costs and insurance burdens.

Detailed Description

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Conditions

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Nasopharyngeal Carcinoma (NPC) Screening Epstein Barr Virus

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SINGLE_GROUP

All participants will be tested for Epstein-Barr virus (EBV) associated biomarkers, including the two-antibody method (VCA-IgA and EBNA1-IgA), BNLF2b total antibodies (P85-Ab), and plasma EBV DNA. Furthermore, novel screening biomarkers, such as next-generation sequencing for EBV and castoff cells using nasopharyngeal swabs, will be explored.
Primary Study Purpose

SCREENING

Blinding Strategy

NONE

Study Groups

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Screening cohort

Participants aged between 30 and 69 years old.

Group Type EXPERIMENTAL

Blood, nasopharyngeal swab and saliva

Intervention Type BIOLOGICAL

Collect blood, nasopharyngeal swab and saliva samples from participants.

EBNA1-IgA, VCA-IgA, P85-Ab and EBV DNA

Intervention Type DIAGNOSTIC_TEST

Detect EBNA1-IgA, VCA-IgA, P85-Ab and EBV DNA for all participants.

Novel screening biomarkers

Intervention Type DIAGNOSTIC_TEST

Next-generation sequencing for EBV and castoff cells using nasopharyngeal swabs, etc..

Endoscopic examinations with or without biopsy

Intervention Type DIAGNOSTIC_TEST

High-risk participants will refer to endoscopic examinations with or without biopsy

Interventions

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Blood, nasopharyngeal swab and saliva

Collect blood, nasopharyngeal swab and saliva samples from participants.

Intervention Type BIOLOGICAL

EBNA1-IgA, VCA-IgA, P85-Ab and EBV DNA

Detect EBNA1-IgA, VCA-IgA, P85-Ab and EBV DNA for all participants.

Intervention Type DIAGNOSTIC_TEST

Novel screening biomarkers

Next-generation sequencing for EBV and castoff cells using nasopharyngeal swabs, etc..

Intervention Type DIAGNOSTIC_TEST

Endoscopic examinations with or without biopsy

High-risk participants will refer to endoscopic examinations with or without biopsy

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Voluntarily signed informed consent.
* Age between 30 and 69 years at the time of screening.
* Residents of Guangdong Province or Guangxi Province.
* Able to cooperate with long-term follow-up.

Exclusion Criteria

* Severe medical comorbidities, significant organ (heart, lung, liver, kidney) dysfunction, or psychiatric disorders.
* Severe autoimmune diseases or immunodeficiency.
* History of or current malignant tumors.
* Inability to cooperate with the study due to psychological, social, familial, or geographical reasons.
Minimum Eligible Age

30 Years

Maximum Eligible Age

69 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Ming-Yuan Chen

OTHER

Sponsor Role lead

Responsible Party

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Ming-Yuan Chen

Professior, Chief physician

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Ming-yuan Chen, MD, PhD

Role: STUDY_CHAIR

Sun Yat-sen University

Locations

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The Fifth Affiliated Hospital of Sun Yat-sen University

Zhuhai, Guangdong, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Ming-yuan Chen, MD, PhD

Role: CONTACT

86-13903052650

Jiong-lin Liang, MD

Role: CONTACT

86-13172018626

Facility Contacts

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Ming-Yuan Chen, MD, PhD

Role: primary

+86-13903052650

Other Identifiers

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ZDWY.BYAFZZX.032

Identifier Type: -

Identifier Source: org_study_id

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