Clinical Trials of Quadrivalent Influenza Vaccine

NCT ID: NCT06824519

Last Updated: 2025-11-20

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE1/PHASE2

Total Enrollment

620 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-05-08

Study Completion Date

2026-09-30

Brief Summary

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This clinical trial adopts a seamless design of phase I/II, conducted in two stages: phase I and phase II. Phase I is the age/dose ramp up stage, and phase II is the dose expansion stage.The purpose of this clinical trial is to evaluate the safety and tolerability of different doses of Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant,explore the immunogenicity of the vaccine, and determine the appropriate dose for later clinical trials of this product.

Detailed Description

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Overall design:

Adopting a randomized, blinded research design. This experiment adopts a seamless connection design of Phase I/II, conducted in two stages: Phase I and Phase II. Phase I is the age/dose escalation stage, and Phase II is the dose extension stage.

Age/dose escalation stage: A randomized, blinded, placebo-controlled study design was used. 120 subjects were enrolled in the study, in order of age from 18 to 49 years old to 50 years old and above, from low dose to high dose. They were divided into four cohorts, with 30 subjects in each cohort (including 20 who received high-dose or low-dose experimental vaccines and 10 who received placebo). The first 6 subjects in each cohort were assigned to be sentinel group, and the researchers reviewed the safety data of the sentinel group within 7 days after vaccination. After confirming safety (not meeting the criteria for suspending/terminating the trial), the remaining 24 subjects in the cohort were enrolled.

Dose expansion stage: A randomized, blinded, positive controlled study design was used. 500 subjects were enrolled, divided into two age groups: 18-49 years old and 50 years old and above. 250 subjects were enrolled in each age group and randomly divided into low-dose group, high-dose group, and positive control group according to a 2:2:1 ratio. The proportion of people aged 60 and above in the population aged 50 and above shall not be less than 80%, and the proportion of people aged 70 and above shall not be less than 30%.

Conditions

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Influenza, Human

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

TRIPLE

Participants Caregivers Investigators

Study Groups

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Phase II low-dose group (18-49 years old)

100 subjects aged 18-49 were enrolled and randomly assigned to the low-dose experimental group, and received one dose of the low-dose experimental vaccine.

Group Type EXPERIMENTAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant (low dose)

Intervention Type BIOLOGICAL

Inject 1 dose of low-dose vaccine

Phase II high-dose group (18-49 years old)

100 subjects aged 18-49 were enrolled and randomly assigned to the high-dose experimental group, and received one dose of the high-dose experimental vaccine.

Group Type EXPERIMENTAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant (high dose)

Intervention Type BIOLOGICAL

Inject 1 dose of high-dose vaccine

Phase II positive control group (18-49 years old)

50 subjects aged 18-49 were enrolled and randomly assigned to the positive control group, and received one dose of the positive control vaccine.

Group Type ACTIVE_COMPARATOR

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent

Intervention Type BIOLOGICAL

Inject 1 dose of positive control vaccine

Phase II low-dose group (50 years old and above)

100 subjects aged 50 and above were enrolled and randomly assigned to the low-dose experimental group, and received one dose of the low-dose experimental vaccine.

Group Type EXPERIMENTAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant (low dose)

Intervention Type BIOLOGICAL

Inject 1 dose of low-dose vaccine

Phase II high-dose group (50 years old and above)

100 subjects aged 50 and above were enrolled and randomly assigned to the high-dose experimental group, and received one dose of the high-dose experimental vaccine.

Group Type EXPERIMENTAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant (high dose)

Intervention Type BIOLOGICAL

Inject 1 dose of high-dose vaccine

Phase II positive control group (50 years old and above)

50 subjects aged 50 and above were enrolled and randomly assigned to the positive control group, and received one dose of the positive control vaccine.

Group Type ACTIVE_COMPARATOR

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent

Intervention Type BIOLOGICAL

Inject 1 dose of positive control vaccine

Phase I cohort 1 low-dose group

20 subjects aged 18-49 were enrolled and randomly assigned to the low-dose experimental group, and received one dose of the low-dose experimental vaccine.

Group Type EXPERIMENTAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant (low dose)

Intervention Type BIOLOGICAL

Inject 1 dose of low-dose vaccine

Phase I cohort 1 low-dose placebo group

10 subjects aged 18-49 were enrolled and randomly assigned to the low-dose placebo group, and received one dose of the low-dose placebo.

Group Type PLACEBO_COMPARATOR

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant placebo (low dose)

Intervention Type BIOLOGICAL

Inject 1 dose of low-dose placebo

Phase I cohort 2 high-dose group

20 subjects aged 18-49 were enrolled and randomly assigned to the high-dose experimental group, and received one dose of the high-dose experimental vaccine.

Group Type EXPERIMENTAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant (high dose)

Intervention Type BIOLOGICAL

Inject 1 dose of high-dose vaccine

Phase I cohort 2 high-dose placebo group

10 subjects aged 18-49 were enrolled and randomly assigned to the high-dose placebo group, and received one dose of the high-dose placebo.

Group Type PLACEBO_COMPARATOR

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant placebo (high dose)

Intervention Type BIOLOGICAL

Inject 1 dose of high-dose placebo

Phase I cohort 3 low-dose group

20 subjects aged 50 and above were enrolled and randomly assigned to the low-dose experimental group, and received one dose of the low-dose experimental vaccine.

Group Type EXPERIMENTAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant (low dose)

Intervention Type BIOLOGICAL

Inject 1 dose of low-dose vaccine

Phase I cohort 3 low-dose placebo group

10 subjects aged 50 and above were enrolled and randomly assigned to the low-dose placebo group, and received one dose of the low-dose placebo.

Group Type PLACEBO_COMPARATOR

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant placebo (low dose)

Intervention Type BIOLOGICAL

Inject 1 dose of low-dose placebo

Phase I cohort 4 high-dose group

20 subjects aged 50 and above were enrolled and randomly assigned to the high-dose experimental group, and received one dose of the high-dose experimental vaccine.

Group Type EXPERIMENTAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant (high dose)

Intervention Type BIOLOGICAL

Inject 1 dose of high-dose vaccine

Phase I cohort 4 high-dose placebo group

10 subjects aged 50 and above were enrolled and randomly assigned to the high-dose placebo group, and received one dose of the high-dose placebo.

Group Type PLACEBO_COMPARATOR

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant placebo (high dose)

Intervention Type BIOLOGICAL

Inject 1 dose of high-dose placebo

Interventions

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Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant (low dose)

Inject 1 dose of low-dose vaccine

Intervention Type BIOLOGICAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant placebo (low dose)

Inject 1 dose of low-dose placebo

Intervention Type BIOLOGICAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant (high dose)

Inject 1 dose of high-dose vaccine

Intervention Type BIOLOGICAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent, ZFA02 Adjuvant placebo (high dose)

Inject 1 dose of high-dose placebo

Intervention Type BIOLOGICAL

Influenza Vaccine (Split Virion), Inactivated, Quadrivalent

Inject 1 dose of positive control vaccine

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

1. When signing the informed consent form, be at least 18 years old and provide valid identification;
2. The subject is able to understand the procedures and methods of this clinical trial, has given sufficient informed consent, voluntarily participated, and signed an informed consent form by the subject themselves;
3. On the day of enrollment, axillary temperature was ≤ 37.0 ℃;
4. Female and male participants of childbearing age: agree to take effective contraceptive measures within 6 months after vaccination.

Exclusion Criteria

1. The laboratory test indicators specified in the protocol are abnormal and clinically significant before vaccination (only for Phase I);
2. Have contracted influenza within the past 6 months prior to enrollment (confirmed by any clinical or microbiological method);
3. Previously or currently suffering from autoimmune or immunodeficiency diseases;
4. Previous history of severe allergies to any vaccine/drug or any component of the experimental vaccine, such as anaphylactic shock, allergic laryngeal edema, allergic purpura, thrombocytopenic purpura, respiratory distress, angioneurotic edema, or individuals with an allergic constitution (such as allergies to two or more drugs, food, or pollen); History of severe allergy to eggs or egg protein;
5. Have received any influenza vaccine within the 6 months prior to enrollment, or plan to receive influenza vaccine other than the vaccine used in this trial during the trial period (before completing the immunization and collecting blood samples);
6. Within 30 days prior to enrollment, any investigational or unregistered products (drugs, vaccines, or devices) have been used, or are planned to be used during the trial period (except for the vaccine used in this trial) (before completing the immunization and collecting blood samples);
7. The interval between receiving attenuated live vaccines before enrollment is less than 30 days, and the interval between receiving other non live vaccines is less than 14 days;
8. Within the first 3 days of enrollment, have experienced acute illness or are in the acute phase of chronic illness;
9. Used antipyretic, analgesic, or anti allergic drugs within 3 days prior to enrollment;
10. Use immunoglobulin and/or any blood products within 3 months prior to enrollment, or plan to use them during the trial period (before completing immunization and collecting blood samples);
11. Long term use of immunosuppressants or other immunomodulatory drugs (defined as continuous use for more than 14 days) within the first 3 months of enrollment, such as a glucocorticoid dose of ≥ 0.5 mg/kg/day (inhalation and local steroid hormones are not restricted);
12. Absence of spleen, functional absence of spleen, and splenectomy caused by any condition;
13. Any obvious coagulation dysfunction or history of anticoagulant therapy;
14. History of epilepsy, encephalopathy, and malignant tumors;
15. Suffering from serious cardiovascular system diseases, serious hypertension with unstable drugs (systolic pressure ≥ 160mmHg and/or diastolic pressure ≥ 100mmHg), diabetes with serious complications and other serious chronic diseases;
16. Currently suffering from respiratory system diseases (including pneumonia, tuberculosis, severe asthma, chronic bronchitis, etc.), acute liver and kidney diseases (severe impairment of liver and kidney function), mental disorders (such as schizophrenia, depression, affective disorders, etc.), etc;
17. Women of childbearing age who are breastfeeding, pregnant, or have a positive urine pregnancy test before enrollment;
18. Plan to move out of the local area before the end of the study or plan to leave the local area for a long time during the study visit;
19. According to the researchers' judgment, the subjects have any other factors that are not suitable for participating in the clinical trial.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Anhui Zhifei Longcom Biologic Pharmacy Co., Ltd.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Fei Jin

Role: PRINCIPAL_INVESTIGATOR

Hebei Province Centers for Disease Control and Prevention

Locations

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Hebei Province Centers for Disease Control and Prevention

Shijiazhuang, Hebei, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Fei Jin

Role: CONTACT

13722795742

Facility Contacts

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Fei Jin

Role: primary

13722795742

Other Identifiers

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LKM-2024-FIn-001

Identifier Type: -

Identifier Source: org_study_id

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