A Probiotic Based Intervention in Pregnancies Complicated by GDM

NCT ID: NCT06794723

Last Updated: 2025-06-06

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Clinical Phase

PHASE2

Total Enrollment

173 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-10-01

Study Completion Date

2029-12-31

Brief Summary

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This study is a single center randomized control trial of a probiotic based intervention in pregnancies complicated by gestational diabetes. A healthy gut microbiome is now recognized as a key component of human health and dysbiosis of the gut microbiome, including lack of diversity, is believed to contribute to the development of many diseases and alter glucose control. The study aims to explore whether this probiotic intervention will improve glucose control and change the gut microbiome. Participants may be enrolled and randomized after diagnosis of gestational diabetes between 24 and 31 weeks gestation. 115 participants will be randomized in a ratio of 2 in the probiotic intervention group to 1 in the placebo group. Participants will stop taking the intervention at 6 weeks postpartum. At this time, they will be unblinded and offered the option of participating in an open-label extension of the intervention until 6 months postpartum.

Detailed Description

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The chosen study design is a single-center, randomized, double-blind, parallel-group, placebo-controlled trial. Participants will be randomized by 2:1 to probiotic intervention or placebo. This is an exploratory study examining the impact of the intervention on glucose control among pregnant persons diagnosed with gestational diabetes.

Participants may be enrolled and randomized after diagnosis of GDM between 240 and 316 weeks gestation. Participants will stop taking the intervention at 6 weeks postpartum. At this time, they will be unblinded and offered the option of participating in an open-label extension of the intervention until 6 months postpartum.

Participants will be seen for up to 4 study visits: 3 mandatory visits in the main study and 1 in the optional extension. Brief visit outlines are below.

Visit 1 at 24-31 weeks gestation:

* eligibility confirmation
* consent
* randomization
* weight, height, blood pressure, method of glucose control from chart
* dispense investigational product
* baseline survey
* pregnancy history questionnaire
* Diet Screening for Adults in Canada (D-SAC)
* Automated Self-Administered 24-Hour Dietary Recall (ASA) x2
* Libre2 1 week of monitoring data
* concommitant medication log
* maternal rectal swab

Visit 2 at 37 weeks gestation:

* weight, height, blood pressure, method of glucose control from chart
* dispense and reconcile investigational product
* D-SAC
* ASA x2
* Libre2 1 week of monitoring data
* concommitant medication log
* maternal rectal swab
* adverse event log

Delivery:

* infant consent
* weight, height, blood pressure, method of glucose control from chart
* delivery chart review
* Libre2 1 week of monitoring data
* weigh placenta
* concommitant medication log
* adverse event log

Visit 3 at 6 weeks postpartum:

* reconcile investigational product
* unblind
* postpartum survey
* D-SAC
* ASA x2
* concommitant medication log
* maternal rectal swab
* infant fecal swab
* adverse event log
* study completion form

Optional Open Label Extension - At 6 weeks postpartum, once all study components are complete, participants will be unblinded and invited to participate in an open label, single arm extension of the intervention till 6 months postpartum. Participants who agree to participate in the extension of the intervention will be provided with additional open-label product until 6 months postpartum.

Visit E1 (open label extension, visit done with V3)

* enroll in extension if desired
* dispense open label product
* maternal and infant consent

Visit E2 at 6 months postpartum:

* Chart review
* D-SAC
* ASA x2
* concommitant medication log
* maternal rectal swab
* infant fecal swab
* adverse event log
* study completion form

Conditions

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Gestational Diabetes Mellitus (GDM) Microbiome, Human

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Participants will be randomized 2:1 to probiotic intervention or placebo. The randomization will be stratified by gestational age at randomization (1) Early - 25+0 to 27+6 weeks and (2) Late - 28+0 to 31+6 weeks. Randomization will be done with random block sizes of 3 and 6.
Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors
For the treatment arms, the study staff, the study participants, and the care provider will be blinded to study arm assignment and will remain blinded until 6 weeks postpartum. At the end of the 6-week visit, once all data collection for that visit is complete, participants and study staff will be unblinded to the group assignment. Participants will then be provided with the option of enrolling in the extension of the intervention until 6 months postpartum. If they consent, additional open label investigational product will be dispensed.

Study Groups

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Probiotic

The investigational product is a 5-strain probiotic plus prebiotic natural health product.

Group Type EXPERIMENTAL

5-strain probiotic plus prebiotic natural health product

Intervention Type DIETARY_SUPPLEMENT

The investigational product is a 5-strain probiotic plus prebiotic natural health product for patients with Type 2 diabetes. Other ingredients include oligofructose, grape food color, magnesium stearate, silica and a delayed release capsule.

Recommended storage is in a refrigerated environment at approximately 4°C. The product should not be frozen. It is recommended that the product be used within 2 months after opening the bottle. Participants are asked to take 2 capsules daily with food, 1 in the morning and 1 in the evening. There is ≥ 2 billion AFU per dose in total.

Placebo

Identically appearing placebo.

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type OTHER

Ingredients include oligofructose, grape food color, magnesium stearate, silica and a delayed release capsule.

Recommended storage is in a refrigerated environment at approximately 4°C. The product should not be frozen. It is recommended that the product be used within 2 months after opening the bottle. Participants are asked to take 2 capsules daily with food, 1 in the morning and 1 in the evening.

Interventions

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5-strain probiotic plus prebiotic natural health product

The investigational product is a 5-strain probiotic plus prebiotic natural health product for patients with Type 2 diabetes. Other ingredients include oligofructose, grape food color, magnesium stearate, silica and a delayed release capsule.

Recommended storage is in a refrigerated environment at approximately 4°C. The product should not be frozen. It is recommended that the product be used within 2 months after opening the bottle. Participants are asked to take 2 capsules daily with food, 1 in the morning and 1 in the evening. There is ≥ 2 billion AFU per dose in total.

Intervention Type DIETARY_SUPPLEMENT

Placebo

Ingredients include oligofructose, grape food color, magnesium stearate, silica and a delayed release capsule.

Recommended storage is in a refrigerated environment at approximately 4°C. The product should not be frozen. It is recommended that the product be used within 2 months after opening the bottle. Participants are asked to take 2 capsules daily with food, 1 in the morning and 1 in the evening.

Intervention Type OTHER

Other Intervention Names

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Akkermansia muciniphila (WB-STR-0001) Anaerobutyricum hallii (WB-STR-0008) Clostridium butyricum (WB-STR-006) Clostridium beijerinckii (WB-STR-0005) Bifidobacterium infantis (Dupont Danisco) chicory root inulin

Eligibility Criteria

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Inclusion Criteria

1. Participants who, in the opinion of the investigator, can and will comply with the requirements of the protocol.
2. Participant has given written consent after study has been explained according to local regulatory requirements and before any study specific procedures.
3. Age ≥16 years at the time of consent.
4. Singleton pregnancy.
5. Live fetus (documented positive fetal heartbeat prior to recruitment)
6. Diagnosis of Gestational Diabetes (GDM) at the time of inclusion (documented 50g glucose challenge test (\>11.1 mmol/L) and/or 75g oral glucose tolerance test with results exceeding the normal range (fasting \>5.3 mmol/L, 1 hour \>10.6 mmol/L, or 2 hour \> 8.9 mmol/L)
7. Willing to provide fecal swab samples.
8. Willing to wear a continuous glucose monitor from enrollment until delivery and for 14 days at 6 weeks postpartum.
9. Willing to provide results from the continuous glucose monitor using the associated app on their mobile device.
10. Willing to complete surveys related to diet, pregnancy history, and health history.
11. Plan to reside in the study area at least until delivery and to deliver at Kingston Health Sciences Center (KHSC).
12. Willing to test for Group B Strep during pregnancy

Exclusion Criteria

Any individual meeting any of the following criteria is not eligible for participation in this study:

1. Current diagnosis of severe gestational hypertension, preeclampsia, HELLP, intrauterine growth restriction, or other clinically significant pregnancy complication(s) at the time of enrollment.
2. Sustained use of substances, such as alcohol, cannabis, nicotine, and other recreational drugs. This is defined as any use after the patient is aware that they are pregnant OR as per the discretion of the investigator.
3. Systemic antibiotic or antifungal use ≤3 months prior to enrollment.
4. Active clinical infection(s), such as sexually transmitted infections, urinary tract infectionss, systemic infections, periodontal disease or positive blood cultures ≤3 months prior to enrollment
5. Acute or chronic clinically significant abnormality or poorly controlled pre-existent co-morbidities, such as autoimmune disease, inflammatory bowel disease (IBD), Crohn's, colitis, or other conditions, that, in the opinion of the investigator, might confound study results.
6. Prescription medications, especially relating to gastric function, or immunosuppressants, that, in the opinion of the investigator, might confound study results.
7. Known hypersensitivity to \>4 first-line antimicrobial therapies against Akkermansia muciniphila, Clostridium beijerinckii, Clostridium butyricum, Anaerobutyricum hallii: Penicillin, Piperacillin, Tetracycline, Amoxicillin, Ampicillin.
8. Known hypersensitivity to \>4 first-line antimicrobial therapies against Bifidobacterium infantis Bi-26TM: Gentamicin, Kanamycin, Streptomycin, Tetracycline, Erythromycin, Clindamycin, Ampicillin, Vancomycin.
9. Any conditions that, in the Investigator's judgement, may interfere with participant's ability to comply with study procedures or receipt of prenatal care, such as behavioural or cognitive impairment or neuropsychiatric illness.
10. Concurrently participating in another clinical study, at any time during the study period, in which the participant has been or will be exposed to an investigational or a non-investigational vaccine/product.
11. Pill swallowing phobia or inability to swallow pills.
12. Not taking any other probiotic supplements during the study intervention period.
Minimum Eligible Age

16 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Queen's University

OTHER

Sponsor Role lead

Responsible Party

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Dr. Graeme Smith

Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Graeme N Smith, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Queen's University

Locations

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Queen's University & Kingston Health Sciences Centre

Kingston, Ontario, Canada

Site Status

Countries

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Canada

Central Contacts

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Jessica Pudwell, MPH, MSc

Role: CONTACT

613-549-6666 ext. 3937

Kira King, BHSc, MLT

Role: CONTACT

613-549-6666 ext. 2740

Facility Contacts

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Jessica Pudwell, MPH, MSc

Role: primary

613-549-6666 ext. 3937

Kira King, BHSc, MLT

Role: backup

613-549-6666 ext. 2740

Other Identifiers

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6043857

Identifier Type: -

Identifier Source: org_study_id

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