Gene Modulation of NLRP3, IL-1β and TNF-α in Peripheral Blood of Patients With Exogenous Obesity Treated With Berberine

NCT ID: NCT06703086

Last Updated: 2024-11-25

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

120 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-01-05

Study Completion Date

2020-02-15

Brief Summary

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The patients will be invited to participate in a research study aimed at determining the Gene Modulation of NLRP3, IL-1β and TNFα in peripheral blood of patients with exogenous obesity treated with berberine.

Detailed Description

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The study will take 6 months in total, in this study will participate patients with exogenous obesity, which is defined as an abnormal accumulation of fat that can be detrimental to health. After the evaluation it will be determined if you meet the section criteria to be able to participate in the study. The patients will be randomly assigned to a group which may be:

Group I. The investigator will give a nutritional plan according to your caloric requirements for lifestyle change and the patients will be scheduled biweekly for 3 months to assess your weight, height, body mass index and percentages of fat or muscle.

Group 2. The investigator will be given a nutritional plan and controlled moderate aerobic exercise that will consist of a goal of 10,000 steps or 150 minutes per week.

The patient will be given a lifestyle change plan in accordance with their nutritional requirements.

Group 3. The investigator will give a nutritional plan, moderate aerobic exercise with a goal of 10,000 steps or 150 min per week; and the phytopharmaceutical Berberine will take 3 tablets every 8 hours for 3 months.

Biweekly sessions will be carried out for 3 months, then monthly for 6 months; blood samples will be taken at the beginning of the study, that is, after the informed consent, at 3 and 6 months.

Conditions

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Obesity Overweight Berberine

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Phase three clinical trial with three intervention groups
Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Nutritional plan only

Nutritional plan according to your caloric requirements for lifestyle

Group Type ACTIVE_COMPARATOR

nutritional plan

Intervention Type BEHAVIORAL

nutritional plan according to your caloric requirements for lifestyle change and you will be scheduled biweekly for 3 months

Nutritional plan and moderate aerobic exercise

nutritional plan and controlled moderate aerobic exercise that will consist of a goal of 10,000 steps or 150 minutes per week

Group Type ACTIVE_COMPARATOR

nutritional plan

Intervention Type BEHAVIORAL

nutritional plan according to your caloric requirements for lifestyle change and you will be scheduled biweekly for 3 months

controlled moderate aerobic exercise

Intervention Type BEHAVIORAL

controlled moderate aerobic exercise that will consist of a goal of 10,000 steps or 150 minutes per week.

Berberine, nutritional plan and moderate exercise

nutritional plan, moderate aerobic exercise and the phytopharmaceutical Berberine 500 mg every 8 hours for 3 months.

Group Type EXPERIMENTAL

Berberine Hydrochloride group

Intervention Type DIETARY_SUPPLEMENT

The patient will be given a nutritional plan, moderate aerobic exercise with a goal of 10,000 steps or 150 min per week; and the phytopharmaceutical Berberine will be taken 3 tablets ( 500 mg) every 8 hours for 3 months.

nutritional plan

Intervention Type BEHAVIORAL

nutritional plan according to your caloric requirements for lifestyle change and you will be scheduled biweekly for 3 months

controlled moderate aerobic exercise

Intervention Type BEHAVIORAL

controlled moderate aerobic exercise that will consist of a goal of 10,000 steps or 150 minutes per week.

No intervention

patients who met the inclusion criteria but did not receive treatment or intervention, only somatometry and blood samples were taken.

Group Type NO_INTERVENTION

No interventions assigned to this group

Interventions

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Berberine Hydrochloride group

The patient will be given a nutritional plan, moderate aerobic exercise with a goal of 10,000 steps or 150 min per week; and the phytopharmaceutical Berberine will be taken 3 tablets ( 500 mg) every 8 hours for 3 months.

Intervention Type DIETARY_SUPPLEMENT

nutritional plan

nutritional plan according to your caloric requirements for lifestyle change and you will be scheduled biweekly for 3 months

Intervention Type BEHAVIORAL

controlled moderate aerobic exercise

controlled moderate aerobic exercise that will consist of a goal of 10,000 steps or 150 minutes per week.

Intervention Type BEHAVIORAL

Eligibility Criteria

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Inclusion Criteria

* • Patients with BMI ≥ 30kg/m2

* With risk factors for type 2 diabetes (history of diabetic parents or siblings, sedentary habits)
* Fasting blood glucose ≤ 126 mg/dL or glycosylated hemoglobin \< 6.5%
* Controlled arterial hypertension
* \>18 years of age
* Accept and sign the informed consent

Exclusion Criteria

* • Pregnancy patients

* Diabetics patients
* Presenting any serious adverse effect
* Patients who decide to withdraw from the protocol
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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National Polytechnic Institute, Mexico

OTHER

Sponsor Role lead

Responsible Party

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Modesto Gómez López

Ph.D., Molecular Biologist,

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Nadia-Mabel PV PhD Nadia Mabel Pérez Vielma, PhD

Role: STUDY_DIRECTOR

044-5520583053

References

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Chuang TY, Wu HL, Min J, Diamond M, Azziz R, Chen YH. Berberine regulates the protein expression of multiple tumorigenesis-related genes in hepatocellular carcinoma cell lines. Cancer Cell Int. 2017 May 30;17:59. doi: 10.1186/s12935-017-0429-3. eCollection 2017.

Reference Type RESULT
PMID: 28572744 (View on PubMed)

Wei W, Zhao H, Wang A, Sui M, Liang K, Deng H, Ma Y, Zhang Y, Zhang H, Guan Y. A clinical study on the short-term effect of berberine in comparison to metformin on the metabolic characteristics of women with polycystic ovary syndrome. Eur J Endocrinol. 2012 Jan;166(1):99-105. doi: 10.1530/EJE-11-0616. Epub 2011 Oct 21.

Reference Type RESULT
PMID: 22019891 (View on PubMed)

Ali AT, Hochfeld WE, Myburgh R, Pepper MS. Adipocyte and adipogenesis. Eur J Cell Biol. 2013 Jun-Jul;92(6-7):229-36. doi: 10.1016/j.ejcb.2013.06.001. Epub 2013 Jun 14.

Reference Type RESULT
PMID: 23876739 (View on PubMed)

Sarjeant K, Stephens JM. Adipogenesis. Cold Spring Harb Perspect Biol. 2012 Sep 1;4(9):a008417. doi: 10.1101/cshperspect.a008417.

Reference Type RESULT
PMID: 22952395 (View on PubMed)

Merino M, Briones L, Palma V, Herlitz K, Escudero C. Role of adenosine receptors in the adipocyte-macrophage interaction during obesity. Endocrinol Diabetes Nutr. 2017 Jun-Jul;64(6):317-327. doi: 10.1016/j.endinu.2017.03.010. Epub 2017 May 16. English, Spanish.

Reference Type RESULT
PMID: 28604342 (View on PubMed)

Fruh SM. Obesity: Risk factors, complications, and strategies for sustainable long-term weight management. J Am Assoc Nurse Pract. 2017 Oct;29(S1):S3-S14. doi: 10.1002/2327-6924.12510.

Reference Type RESULT
PMID: 29024553 (View on PubMed)

Siani A, Cappuccio FP, Barba G, Trevisan M, Farinaro E, Lacone R, Russo O, Russo P, Mancini M, Strazzullo P. The relationship of waist circumference to blood pressure: the Olivetti Heart Study. Am J Hypertens. 2002 Sep;15(9):780-6. doi: 10.1016/s0895-7061(02)02976-x.

Reference Type RESULT
PMID: 12219872 (View on PubMed)

Peralta-Romero Jde J, Gomez-Zamudio JH, Estrada-Velasco B, Karam-Araujo R, Cruz-Lopez M. [Genetics of pediatric obesity]. Rev Med Inst Mex Seguro Soc. 2014;52 Suppl 1:S78-87. Spanish.

Reference Type RESULT
PMID: 24866313 (View on PubMed)

Al-Goblan AS, Al-Alfi MA, Khan MZ. Mechanism linking diabetes mellitus and obesity. Diabetes Metab Syndr Obes. 2014 Dec 4;7:587-91. doi: 10.2147/DMSO.S67400. eCollection 2014.

Reference Type RESULT
PMID: 25506234 (View on PubMed)

Heilbronn LK, Campbell LV. Adipose tissue macrophages, low grade inflammation and insulin resistance in human obesity. Curr Pharm Des. 2008;14(12):1225-30. doi: 10.2174/138161208784246153.

Reference Type RESULT
PMID: 18473870 (View on PubMed)

Provided Documents

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Document Type: Study Protocol and Statistical Analysis Plan

View Document

Document Type: Informed Consent Form

View Document

Other Identifiers

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No.ESM.CE-01/7-12-2015

Identifier Type: -

Identifier Source: org_study_id

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